We narrowed to 633 results for: gats

...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...Always be sure to include various positive and negative controls to “convince [any] old skeptic professor...

...created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual G protein signal transducers...TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat Chem Biol 16:841...

...cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is...

...biomedical research community, but also allows cross-interrogation of different datasets that might otherwise be...to identify. Figure 2: Users can cross-interrogate screens from the literature for a drug, disease...

...including replications and experiments resulting in “negative data” often dominate the early years of a project...recommend sharing ReFigures on your Linkedin and ResearchGate profile. To see how to create ReFigures in a...

...both in brain slices and freely behaving mice to gather the following types of data: Report time-course...and mouse brain slices and freely moving mice to gather the following types of data: Report endogenous...

...thermophiles (high OR medium heat loving), but not obligate thermophiles. However, the recent discovery of... CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to grow the organisms...

...stayed to see the Opening Keynote address with Bill Gates. The long line for bag check was definitely worth...for global health and the various initiatives the Gates’ Foundation is involved with was one of the highlights...

...possible pitfalls in your strategy and how can you mitigate them? If you were to write a grant for this project...project along the way. Advice from principal investigators Tom Ellis highlights the importance of keeping...

... focus on how to choose an advisor/principal investigator (PI) that will also serve as a good mentor. ...use publication records, university websites, ResearchGate and LinkedIn to find past lab members. Most ...

...partial loss of an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging...packaging. To prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient bacterial...

...advancing our knowledge in areas such as bacterial conjugation and recombination, replication and topology, ...Central PMCID: PMC1209583. Watanabe, Tsutomu, Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF...

...Transcription factors (TFs) act as the gate keepers for gene expression, turning transcription on and...individual plasmids from the library to further investigate specific genes and TF isoforms. Due to the size...

...funded a center for mitochondrial research to investigate the scientific possibility, safety, and efficacy...efficacy of the mtDNA transfer options being investigated; A UK regulatory body tasked two separate non-governmental...

...followed these experiments with more detailed investigations into the release of DNA post-cut. Using substrate...Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab ...

...kinase. This hybrid construct senses light, and an obligate chromophore allows the system to respond to various...photosensor senses various light inputs, and an obligate chromophore allows the system to respond. In far-red...

... blog post, we covered how to use positive and negative selection in plasmid cloning.  But oftentimes...experiment, you’ll want the proper positive and negative controls for your PCR.  Find the details and learn...

...editing speed dictates which phage variants can propagate faster than the rate of dilution (passage from...off-target DNA and off-target RNA activity. To mitigate this drawback, the lab introduced an additional...

...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb...

...mutations and truncations that can make vector propagation and sequencing a headache. How do I maintain ...integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth culture...