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Addgene
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  2. Science as Art

    Type
    Blog Post
    ...have been enchanting people for years and have gathered much acclaim - including being featured in the...smaller competitions are now taking place and gathering momentum, including in Cambridge and Bristol. ...

  5. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...Always be sure to include various positive and negative controls to “convince [any] old skeptic professor...

  7. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...both in brain slices and freely behaving mice to gather the following types of data: Report time-course...and mouse brain slices and freely moving mice to gather the following types of data: Report endogenous...

  10. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...thermophiles (high OR medium heat loving), but not obligate thermophiles. However, the recent discovery of... CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to grow the organisms...

  11. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual G protein signal transducers...TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat Chem Biol 16:841...

  12. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...stayed to see the Opening Keynote address with Bill Gates. The long line for bag check was definitely worth...for global health and the various initiatives the Gates’ Foundation is involved with was one of the highlights...

  13. Advice for Choosing a Research Project

    Type
    Blog Post
    ...possible pitfalls in your strategy and how can you mitigate them? If you were to write a grant for this project...project along the way. Advice from principal investigators Tom Ellis highlights the importance of keeping...

  14. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ... focus on how to choose an advisor/principal investigator (PI) that will also serve as a good mentor. ...use publication records, university websites, ResearchGate and LinkedIn to find past lab members. Most ...

  15. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...partial loss of an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging...packaging. To prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient bacterial...

  19. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...mutations and truncations that can make vector propagation and sequencing a headache. How do I maintain ...integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth culture...

  20. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...followed these experiments with more detailed investigations into the release of DNA post-cut. Using substrate...Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab ...
Showing: 281 - 300 of 625 results