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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 328 results for: hal.1

Showing: 281 - 300 of 328 results

  1. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...email Ryan at [email protected]   References 1. Larivière, Vincent, Yves Gingras, and Éric Archambault...solutions to complex challenges. As a cross-disciplinary researcher, the challenge of keeping up on studies... Sharing your research with the world can be challenging. After months, or years, of grueling effort to...biology or international relations.  Many of the challenges in science today are too complex for any single...making publishing their research all the more challenging even when it is of quality). On the site you ...

  2. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...therapies to clinical trials in 2021. Figure 1: Schematic of the human MECP2 locus and MECP2 protein...cassette using tamoxifen-inducible Cre not only halted the neurological damage but completely reversed... Rett Syndrome, respectively. One of the main challenges facing Rett researchers is how to deliver the...adeno-associated viral (AAV) vector. The editase repaired half of the mutant MECP2 RNA in each of three neuronal... by Gail Mandel. There are numerous technical challenges to overcome for both of these approaches, such...

  3. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...yourself in a creative rut, read on!   Fig 1: Blugene using creative problem-solving in the lab... needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding ... review it. This survey generated 26 ideas, over half of which, when implemented, impacted the entire ...you can, set aside time to prepare for future challenges. This doesn’t have to be time-consuming or even... keeping your science going throughout these challenging times.  Take a Break! If all else fails, one ...

  4. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ... education and scientific writing.     References 1. Martz, Colin A., et al. "Systematic identification...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...faster. Years of science, done in weeks Figure 1: Sending out 80 FedEx envelopes in a single day --...hijacking of human cells. Even better, more than half the drugs hitting these proteins are FDA-approved...

  6. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...the guides are relatively unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences...guide sgRNA-Sirius-8XMS2 which will recruit the HaloTag with red fluorescent ligands to the loci of interest...

  7. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...regarding what makes this group of researchers special: 1. Stem cell scientists care deeply about ethics Ethics...trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic due to a spinal cord...

  8. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... will be a positive asset throughout your career? 1. Make scicomm practice a priority The good news for...of science, communication isn't easy.  But the challenge is worthwhile and rewarding. A truly passionate...

  9. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...into account, the digest pattern depicted in Lane 1 would be expected for a XbaI/NotI digest. The program...plasmids. It turns out that restriction enzymes are one half of naturally occurring restriction modification ...

  11. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice were histologically...gRNA together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb...transgenes might seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In...WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang...

  12. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze... Biology (Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their...experience on their resumes). CUREs are not without challenges. Real research is messy, and it can be difficult...

  13. Bioinformatics at Addgene

    Type
    Blog Post
    ...drives our bioinformatics needs       Fig. 1: QC'ing data at Addgene is like standing under a Niagara...300,000 individual nucleotide sequences. More than half of these sequences were generated by Addgene as ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to...

  14. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ... UK team members before they log off for the day. 1:45 PM--Write final report. Work on a final report ...then go to grad school. However, about a year and a half into my role, I realized that actually, I didn’t...

  15. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...efficiently with adenovirus and AAV.   Table 1: Types of viruses and their features Choosing ...However, the cargo capacity of AAV is ~4.7 kb, almost half that of lenti and gamma-retro viruses. This is large...

  16. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...accessible to practically everyone.     Fig. 1: Open source hardware builds   Since I started...hardware, this process is often much more involved. Challenges such as the provision of an accessible supply...

  18. Summer SciComm: Preprints

    Type
    Blog Post
    ...medRxiv.org) in the past few years.   Figure 1: Stand aside, CERN — it's a new age of accelerating...Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (2022) A guide to preprinting for...

  19. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...with each protein of interest.    Figure 1: The direct approach (left) versus the indirect approach...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...

  20. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination. I. ...in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology,...
Showing: 281 - 300 of 328 results