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Showing: 281 - 300 of 878 results

  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...Interested in using PTG plasmids in your research? Check out the PTG plasmids or plant expression systems...common to prokaryotes and eukaryotes. Though the plasmids made by Xie et. al are specifically designed for....1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication at Addgene Other Techniques....2014.04.022. Epub 2014 May 15. PubMed.  Find the plasmids from this publication at Addgene Dimeric CRISPR...nbt.2908. Epub 2014 Apr 25. PubMed.. Find the plasmids from this publication at Addgene ...

  2. Typing CRISPR Systems

    Type
    Blog Post
    ...diverse and involved in competition between plasmids. Nucleic Acids Research, 48(4), 2000–2012. https://doi.org...recently that researchers began building optimal plasmids to harness the power of Class 1 systems. Class...Lastly, type IV systems are most often found on plasmids. The many unique features of IV-A and IV-B have...among the smallest Cas enzymes (400 to 700 amino acids), use a Cas14 enzyme, and uniquely target single-stranded...evolution of type II CRISPR-Cas systems. Nucleic Acids Research, 42(10), 6091–6105. https://doi.org/10.1093... resources on Addgene.org CRISPR guide CRISPR plasmids CRISPR references ...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ...that is required is that the proteins/nucleic acids/lipids of interest are fluorophore labeled and that...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that have mastered...pyrrolysyl-tRNA synthase for aliphatic diazirine amino acids E. coli and Mammalian Cells pEVOL-pAzF synthetase...Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have...

  4. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post
    ...Richard Gronostajski who each are being awarded free plasmids form Addgene to accelerate their research. Read... on to learn how these researchers plan to use plasmids from Addgene to advance their research on diseases...Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune cell...change protein sequence affect arginine (Arg) amino acids more frequently than any other. Indeed, nearly 20%...individuals worldwide.Arg is unique among amino acids in that it possesses a guanidine moiety at the end...Nfix-negative neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors...

  5. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post
    ...systems.   Four years later, the Sontheimer and Davidson labs discovered three anti-CRISPRs for a type ...tissues The small size of Acr proteins (~50-200 amino acids) means they can be delivered in vivo using AAV vectors...Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA (2017) A Broad-Spectrum..., Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR (2016) Naturally Occurring Off-Switches for...CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible...light-inducible Cas9 activity? Check out these these plasmids Catch Up on Your CRISPR Background with Our Guide Pages...

  6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...slight variations in their viral capsids. Novel forms of AAV capsids are discovered frequently and recent...developing new capsids through direct evolution, capsid shuffling, and peptide displays. These capsids will be...enabled us to generate new and refined forms of capsids with improved specificity. AAV vectors are able...clinic Thus far, AAV vectors AAV1, AAV-2, AAV1-AAV2 hybrids, AAV-6, AAV-7, AAV-8, AAV-9 and AAVrh10 have been...

  7. Genome-wide Screening Using CRISPR

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    Blog Post
    ...are composed of many different gRNA-containing plasmids, each with a different genomic target. The goal...sequences and ease of generating gRNA-containing plasmids. CRISPR genome wide-screening experiments commonly...sequencing - CRISPR libraries contain thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid...other types of experiments, check out our CRISPR Plasmids and Resources Page. References McDade JR, Waxmonsky...Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control for Pooled Libraries ...More CRISPR Resources at Addgene: Find CRISPR Plasmids Read our CRISPR Guide Find tools to design your...

  8. Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!

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    Blog Post
    ...100,000 different plasmids available from the repository (currently at 98,276 plasmids as of April 8, 2021...re now sharing over 100,000 reagents including plasmids, ready-to-use viral preps, pooled libraries, kits... 2021) 1.5 million plasmids shared in total (currently at 1,484,507 as of April 8, 2021) 5,000 depositing...addgene.org homepage! You can track the number of plasmids right below the search bar and more stats further...

  9. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post
    ... on Addgene.org Find plasmids from David Liu's lab Visit our COVID-19 plasmids and resources ...22/14 to 5/19/20). Each paper has between 1–32 plasmids and a total of 2–2,590 requests. We received plasmid...by date. Parentheses depict the total number of plasmids ordered for each paper. Rough estimation of...observed that the demand for the corresponding plasmids tended to follow an exponential decay model, with...places an order, they are likely to order several plasmids at once, breaking the statistical independence...

  10. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post
    ...nucleases, AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered pattern...system." Cell (2015). PubMed PMID: 26422227. Find plasmids from this publication at Addgene. 2. Zetsche,....1 (2016): 31-34. PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik...Biotechnol. (2016). PubMed PMID: 27272384 Find plasmids from this publication at Addgene. Kleinstiver...Biotechnol. (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. Makarova, Kira...PMID: 26411297. Resources at Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide...

  11. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... no kids. Get a massage – no guilt. Have recreational time with your friends – no spouses or kids. The...will suffer. And, yes, your partner CAN take the kids to the doctor without your help. Your partner is...a family with working parent(s), the sooner your kids can make a decent meal, fold their laundry, take...things can wait and some things can’t. I kiss my kids good night every evening I am home no matter how...me, that’s been volunteer work at our temple, my kids’ school, and with professional organizations supporting...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ...construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters...sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular application...

  13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species...additional RNA-binding transcriptional activator. Plasmids for activating target genes in a variety of species...systems Addgene carries a variety of Cas9 containing plasmids that have been optimized for expression in different... plants, drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on... resources on the Addgene website Find CRISPR Plasmids Catch up on your CRISPR background with our CRISPR...

  14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ....org Find Cre-Lox Plasmids Find Optogenetics Plasmids Find Chemogenetics Plasmids ...labeling of individual neurons one could reduce the plasmids amounts to reduce transfection efficiency. However...visualized at the same time from two different plasmids this approach becomes problematic as it reduces...that most cells are transfected with all labeling plasmids and the low concentration of recombinase restricts...

  15. DIY DNA Ladders from Penn State University

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    Blog Post
    ...developed the plasmids, pPSU1 and pPSU2. When restriction digested with PstI or EcoRV, these plasmids generate...Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently...prep protocol here and the pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze...., Johnston, J. L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific...

  16. Streaking for Single Colonies: The Streak Plate Challenge

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    Blog Post
    ... Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids at Addgene Check out additional...other species.  When he found the chromoprotein plasmids available from Addgene, he knew it would be a ...molecular biology. “What I'd like to do with the plasmids from Addgene is have the students transform them...plate skills? Find the chromoprotein expressing plasmids here, and check out our streak plate protocol ...

  17. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...specific names of the plasmids you plan to use Enter the base pair size of those plasmids Enter plasmid concentration...packaging typically requires transfecting three plasmids at specific molar ratios. To get these ratios ...RepCap added together) per 15 cm dish or 2500 μg of plasmids per CF5 stack. Total ug of each plasmid needed...on Addgene.org Find AAV Packaging and Transfer Plasmids Here New to Making AAV? Check out Addgene’s Step-By-Step...

  18. Five Popular Model Organisms

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    Blog Post
    ... resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of mice as model... pancreas. They first tagged these cells using plasmids. Then using HOTcre, a method that uses heat induction... feeding the worm bacteria expressing specific plasmids (L4440) that contain your gene of interest (Fire...al., 2015). If you are interested in C. elegans plasmids and resources check out Addgene’s Worm Expression...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...pages Read our molecular biology reference Find plasmids for your research ...

  19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post
    ...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Find Plasmids from the Whitehead Lab Use... a selective pressure winnows the pool down to plasmids expressing variants with optimal function. High-throughput...be used to generate complex libraries of mutant plasmids ready for deep mutational scanning.     3 reasons...favorite plasmid with this protocol? Maybe. Not all plasmids will work for the one-pot mutagenesis method. ...Mutagenesis to Generate Specific Mutations in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries ...

  20. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...immunodeficiency syndrome (AIDS) in untreated individuals. Symptoms of AIDS include rapid weight loss ...26538064. PubMed Central PMCID: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski...PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, ...strategies: potential tools for eradicating HIV-1/AIDS. J Neurovirol. 2015 Jun;21(3):310-21. PubMed PMID...Addgene’s newly updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...
Showing: 281 - 300 of 878 results