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We narrowed to 871 results for: lic

Showing: 281 - 300 of 871 results

  1. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... our knowledge. Disease Applications Q13: Does AAV have potential applications in diseases which may be... to use split-inteins to mediate trans-protein splicing. Note, however, that this will require co-delivery...reliable and reproducible. However, we do not use amplicons in ITR as we have found this to strongly bias ...1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011 - 1x1012 vg/...CRISPR tools is an exciting area for future AAV application to epigenetic editing. This area is discussed...

  2. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...protein for your application. Selecting the best fluorescent protein for a specific application can seem like...under conditions that best mimic the intended application. The three key properties for live cell imaging...experimental set up. The practical brightness takes all application specific parameters into account, including the... picture of what can be expected in a 'real' application than the theoretical brightness. Consequently...brightness in a system closely mimicking the future application. In figure 2 we provide an example of the comparison...

  3. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...transcription, or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does...with Addgene, but you’ll find many great tools by clicking on the links below and visiting their associated...containing lists of plasmids from the indicated publications. You can request all of these plasmids directly..., Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen ..., Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and...

  4. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...tool that can be used for a variety of research applications. Within the immune system, antibodies are produced...antibodies to bind proteins is useful for research applications as well because they allow scientists to target...150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies You can use antibodies for experiments...Let’s dive in and take a look at some of these applications. Western blot Western blots are used to detect...immunocytochemistry). This could be cells or tissue slices mounted to a slide, or whole organs. Because the... by binding to cell surface receptors. These applications are typically used for investigating signaling...are out there, and how many different research applications they can be used for. Addgene’s Antibody Plasmid...

  5. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...ampicillin resistance cassette and an origin of replication, which are not schematized. Sizes of fragments...2024, used under the Creative Commons CC BY-NC-ND license.   Once you have the components you want, simply...validated and were chosen because of their broad applicability in different model systems. What’s the error...five months. When they tested those clones (in duplicate), 93% had the correct banding pattern in the restriction...correct. Because they created the assemblies in duplicate, they were able to confirm that at least one plasmid...design. Note that you can browse all components by clicking the blue text in the upper right-hand corner.  ...

  6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...chemical replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...inheritance that might be less error prone than DNA replication. He too mentioned how “surprising” small RNAs...created a circRNA website so scientists can view public circRNA datasets and download the custom python...Steitz quipped that RNA in the ribosome is in a complicated conformation “not like DNA which has a boring...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...delivered with an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  ...2024 bioRxiv, under CC-BY-NC-ND 4.0 International license. With this approach, the team was able to drive...Arabidopsis, suggesting the improvements may be broadly applicable to dicot species. In addition, they generated...Adapted from Smith et al. 2024 under a CC-BY 4.0 license.  The team deposited an array of constructs...activation triggered by endogenous GPCR. Example applications include large-scale pharmacological profiling...adapted from Wu and Wang 2024 under a CC-BY 4.0 license. Find more neuroscience antibodies here! ...

  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...are, you’ll even have DNA left over for other applications, like PCR, cloning, transfection, or long-term...wash step or use a different strain. For most applications, Addgene uses and recommends DH5𝝰! This strain...your E. coli to ensure it is suitable for your application. We have previously reviewed many common lab ...partially specified by a plasmid’s origin of replication, but can also be affected by the plasmid size...the material used for inoculation. To further complicate matters, plasmid copy numbers are often ambiguous...volume limits, the copy number of some origins of replication (e.g. p15A, ColE1) can be increased by adding...

  9. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...about a month and a half, without the need for complicated cloning of homology arms. MMEJ: An Introduction... inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific... for making targeted modifications. A recent publication by Zhang et al. shows just that - using MMEJ ...lies in the diversity of nucleases and their applications. It will be interesting to see what new editing...PMID: 26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015...633. PMID: 26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei...

  10. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...production system may depend on what downstream applications you are planning, cost, and the amount of protein...accurate concentration to give your downstream applications the best chance at being successful.  Using ...they bind their antigens specifically. Possible applications for validation include western blot, immunohistochemistry...some antibodies are better suited for certain applications. An antibody that performs well in immunohistochemistry...use! You can learn more about common antibody applications in our Antibodies 101 blog series.  Resources...H, Zhang Y, Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale...

  11. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ... primarily on fluorescent proteins and their applications, tackles much broader issues in this brief volume...sexism in science, problems with peer-reviewed publications and funding, bioethics in the age of CRISPR,...to the steadily increasing distrust the general public has in science and scientists (as discussed in ...Readers unfamiliar with academia’s challenges with publication and funding may be dismayed to see the dirty ...CRISPR of course address some of the ethical implications of using these technologies, but also the rapidity...and the marginalization of science, particularly public health and environmental science, by the Trump ...

  12. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...emerging work by collaboratively discussing recent publications in a specific field(s). Most anyone who has ...major findings and then focusing more on broader implications rather than diving deep into the individual ....  These variations are suited for different applications and should be carefully chosen for each group...first meeting, now what? After the first meeting solicit feedback. To ensure that the journal club is useful...wanted, but were unable to attend for scheduling conflicts. This helped us find a time that accommodates ...accommodates more people. The feedback could be solicited via multiple avenues including casual request via email...

  13. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...-target editing in a cell culture model. The application of Cpf1 to genome editing is exciting both in...in terms of basic science and translational applications. This discovery of this type V CRISPR system ...PubMed PMID: 26422227. Find plasmids from this publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex...PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik, et al. Genome-wide...PubMed PMID: 27272384 Find plasmids from this publication at Addgene. Kleinstiver, Benjamin P., et al....PubMed PMID: 27347757. Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...use, functional in most species, and has many applications. That said, SpCas9 is not the only game in town... makes this molecule unique, and the various applications of Cas13a. The origins of Cas13a: An RNA cleaving...discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   Figure 1: Using Cas13a as...of nucleotides.   What are the potential applications of Cas13a given what we know about its structure...fusion proteins for imaging, tracking, modulating splicing, and regulating expression of specifically targeted...CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was originally published...

  15. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... Aptamer biosensors are used in a variety of applications, including detection of environmental pollutants... opens aptamers for use in many entirely new applications including aptamer based fluorescent biosensors... render this approach less sensitive in most applications. Learn About Fluorescent Biosensors Light-up..., MG proved to be too toxic for many in vivo applications, but this work proved the concept that RNA could...and biochips. Ultimately, there are just some applications that lend themselves to aptamer-based instead...Kyung-Mi, et al. “Aptamers and Their Biological Applications.” Sensors (Basel). 2012; 12(1): 612–631. PubMed...

  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...each sample by clicking on the sample name or on its corresponding bar graph entry. Clicking to initiate ...sequencing data alongside the analysis of NGS-based amplicon sequencing data and found that the accuracy of...100-fold reduction in cost relative to NGS-based amplicon sequencing. To use the tool, you start by uploading...are no parameters that need optimizing and no complicated steps to learn. For increased flexibility and... download the entire analysis as a ZIP file by clicking the Download Analysis Data button on the bottom...

  17. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...investments over the next 3, 5, or 10 years. Complicating these challenges is the fact that they occur...chemotherapies, or developing innovative mobile phone applications and services that help diabetic patients manage...collaborative work place, challenges can arise due to conflicting work styles, team situations, and personalities...literature, and critical analyses skills were applicable to my cases. I can directly use my understanding...the gap between the science and the commercial applications of a particular therapy. With your scientific...conversations, reading online healthcare industry publications like FiercePharma, FierceBiotech, and BioCentury...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...strategies Publication System nickname Photoactivatable moiety Cas9 variatn CRISPR protein application Split...improvements to CRISPR technology since its initial applications for genome engineering in 2012.  Many of these...particular interest since blue light has limited applications in vivo. UV and blue light is readily absorbed..., allowing the release of Cas9. A noteworthy application of the CASANOVA system was its use in light-mediated...continue to evolve and can’t wait to see what cool applications you use these for in the future! References...Bouazza B, Schumacher CH, Bratovič M, Charpentier E, Möglich A (2013) Engineering of temperature- and light-...

  19. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...include understanding of allelic variation between different plant species and metabolic properties affecting... ease of manipulation and their ability to be replicated. Because E. coli are easily propagated and have...minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive...Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...

  20. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... do need multiple first/corresponding author publications in quality journals. It helps to come from the...a job. The currency of scientific success is publications and grants. 2. Write succinct and easy to understand...Attendees will include grad students (that are solicited for feedback), possibly the Dean, and even some...essential for human health. Failure to do so is implicated in several human diseases. Proper patterning ...disease treatment, etc., again in clear terms. "Implications for human disease" is vague. Instead, consider...interviews. For point of reference, I submitted my applications all by snail mail and only had phone interviews...
Showing: 281 - 300 of 871 results