...tools are not very robust. When using GCE, each component of the experiment system (including media, cell...optimization parameters the same for all three components, meaning that the final protocol may involve ... They provide resources, knowledge, and most importantly, validated protocols (using readily available...

...gamma-retrovirus are so closely related, their required components are very similar. While plasmids containing virus-specific...between the two systems, other, more general, viral components such as a heterologous envelope or a post-transcriptional...713. DOI: 10.3390/v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of the Application...

...the target cell or transiently expressed. It’s important to note that for all the applications we discuss... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...

...permanently integrate into the host genome or temporarily express whatever it encodes. This ability to ...allows you to infect cells and deliver cargo, it’s important to understand their differences to choose the ...PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human immunodeficiency virus type 1 preintegration...

...consumer-grade components. In this field, often referred to as 'frugal science', existing components are adapted...around the use of commercially available tools and components. Rapid prototyping techniques such as 3D printing...

...aging. For both a screen and a selection, it is important to clearly define what type of factor you are ...CRISPR, drug, etc.), just with an added baseline component. Alternative screening techniques While CRISPR...whole genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you ...

...severely cold conditions. To understand their unique importance, let’s begin with a few basics.  Mesophiles vs...understanding and appreciating their biology. The importance of psychrophilic enzymes From an industrial perspective...scientist from Addgene. The talk highlighted the importance of reagent repositories and the myriad facilities...

... Our color changing flower involves two basic components: the pigments (anthocyanins), and the plant circadian...also identified and characterized mechanisms and components of the circadian rhythm in petunias. Because ...before us, it’s possible for us to combine these components to develop flowers that change color. Without...

...in the lab typically contain all the necessary components to allow shuttling between E. coli and yeast ...an organism to synthesize a particular organic compound required for its growth. Many auxotrophic strains... be used with a yeast strain deficient in that compound. Therefore known auxotrophic strain/ selection...

... non-linear dependence on excitation power is important since different fluorescence imaging strategies...requirement for quantitative live-cell imaging, it is important to be able to quantify the photostability of fluorescent.... To avoid changes in excitation power, it is important to perform the experiments on a single day on ...

...specific chromosome. This image represents the composite of fluorescent measurements from red, blue, and...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA...CRISPRainbow generates 6 unique colors and a white composite. gRNAs with two identical hairpin binding domains...

...specific components that are key to the cloning reaction. Understanding the specific components is essential...understanding all of the players becomes ever more important to ensure that you choose the most appropriate...

...2017). However, Acr inhibition is spatially and temporally ubiquitous, making them a rather blunt instrument...versatility opens up the potential for spatial and temporal control in many genetic targeting and editing ...response to light, offers a way to spatially and temporally control cellular processes in vivo. Many photoactive...

...wider group of researchers. Generating material to empower others to practice and teach reproducibility An... exclude different modules for this workshop. Empowering others to have the materials and resources to...External Collaborations Learn about the 2018 Minisymposium on Scientific Reproducibility Read a blog post...

...The Rare Genomics Institute BeHEARD (Helping Empower and Accelerate Research Discoveries) Challenge provides... and induce their effector functions. It’s an important process that we need to understand so that we ...source of IL-36 cytokines in the skin making it important to study what is happening within the keratinocytes...

...thesis as it is for helping your team reach an important milestone at work.   While I was in graduate school...work through it. Ask yourself if you missed any important details. Or challenge yourself to think of more...that this skillset is a part of your toolkit is important. Paired with your other skills, both technical...

...for IgG subtypes from different animals so it’s important to choose the reagent that works for your species...is no more of that exact polyclonal antibody composition available. If you’re going to need a large quantity...sure the staining you are seeing is real. It’s important to include a negative control using serum from...

...results indicate that the tag protein may have an important influence on the expression of the transgene of...fused to mCherry. But our data does highlight the importance of a careful characterization of transgene expression... or chemogenetic receptor. This may not be an important limitation in many studies, and could even be ...

...terminology of a new field. Annotate: When creating important documentation, including SOPs or project-related...Product Manager role at Addgene is “Words are important!”  If you try any of these approaches in your ...propensity for cross team communication, that’s an important soft skill to carry into the next phase of your...

...biology known as bioelectricity is revealing the important role of bioelectrical signaling mediated by ion-channels...attended the Developmental Bioelectricity satellite symposium at the 2019 Society for Developmental Biology ...Center at Tufts Developmental Bioelectricity symposium’s “What is Bioelectricity?” Journal of Bioelectricity...