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We narrowed to 658 results for: otos

Showing: 281 - 300 of 658 results

  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...infection. Dapeng Zhou Other Resources Protocols Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral... Spike Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Addgene Blog ...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...basic-hFX-promotor(1kb) FXN hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN...basic-hFX-promotor(2,5kb) FXN hFXN Friedreich ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal... DCTN1 CMV ALS, motor neuron disease Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS, motor neuron disease Trina... ALS, motor neuron disease Caren Norden 105971 pME mKate2-dynactin1-811 DCTN1 mKate2 ALS, motor neuron...Aebischer 36154 pEGFP-p150Glued DCTN1 GFP CMV ALS, motor neuron disease David Stephens 36382 pSMP-DNMT1_1... Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS, motor neuron disease Trina Schroer 51435 pGL3Basic_ME.1/ApoEpromoter...Charlet-Berguerand 74170 pVEX-CC1 DCTN1 tac ALS, motor neuron disease Trina Schroer 75437 p-AAV-sh[SNCA...

  3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...a proxy for autophagy. Cells are cultured with isotope-labeled amino acids for several hours to several...Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy Additional...

  4. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ... with most multi-labeling immunohistochemical protocols where either a directly labeled primary or a primary...secondary antibody. Compatible with multi-labeling protocols when other antigens are detected with either a...

  5. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ... saves time and money when developing assays.  Protocol architecture The basic (very basic!) architecture...increased binding affinity, many indirect antibody protocols are designed to take place over multiple days....

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...become slightly denatured during your experimental protocol, using a polyclonal antibody increases the likelihood...Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org/10.1101/pdb.top099101...

  8. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    .... Use the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn...for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip: Turn...

  9. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...has  developed a proprietary, low cost cloning protocol that he has used for cloning of more than 10,000...plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our molecular biology...

  10. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...engineers in your lab, you likely will need to write protocols, SOPs, and methods sections. The next time you...academic labs when creating shared experimental protocols. Clarify: Asking for clarification during a meeting...

  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... RNA editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting...development and use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting...

  13. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... Their goal was to figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA... a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit the...

  14. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...open-source' protocol for making customized zinc-finger arrays. Maeder ML et al. Nat Protoc. . 2009. Sept...customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(12):1855-1867. PubMed PMID 20010934...

  20. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...structures, etc., as well as the presence of a Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence...genome editing in stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI:10.1038/nprot.2016.171 ...
Showing: 281 - 300 of 658 results