...across systems and species. The cost? Zeocin is genotoxic when not 100% inhibited, meaning it will induce...Addgene.org Addgene’s Basic Molecular Biology Protocols Over-Agar Antibiotic Plating Plasmids 101 eBook...

...adenovirus type 5 (hAdV-C5) can be considered the prototype, although many viruses infecting humans (from ...oxfordjournals.aje.a113563. PMID: 6301263. Servellita, V., Sotomayor Gonzalez, A., Lamson, D. M., Foresythe, A., Huh...

... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted... time, etc., which can vary even when the same protocol is being followed.  Finally, standard curves must...

...resources: plasmid, pooled library, blog posts, protocols, and more. Selecting crispr in Guides will return...about the subject, link to related guides and protocols, and provide easy to sort tables of hand-picked...

... incubated together to form the RNP. Detailed protocols outlining these steps have been made publicly ...Polyethylene Glycol (PEG) mediated transfection of protoplasts and biolistic bombardment of immature embryos...

...that increased the fluorescence intensity and photostability of GFP. This also shifted its major excitation...Known Function S65T Increased fluorescence, photostability, and a shift of the major excitation peak to...

...increased particle stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral...Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and Resources More Lentiviral FAQs Answered by...

...antibody new tricks By Ashley Waldron While IHC protocols typically call for thin sections of tissue, the...perform well in standard IHC and western blot protocols. You can find more specific information on the...

...virus properly and be consistent when following protocols. Need Virus? Check out Addgene's New Viral Service...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 7....

... for Protein Expression in Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed...Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic Restriction Digest Video ...

...Resources at Addgene.org Check out our Protocol Pages for Video Protocols ...

...-I3, while initially dimmer, showed greater photostability and also matured considerably faster, with ...abscessus, an increasingly common human pathogen notorious for its high resistance to antibiotics. Several...

...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

...Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by ...combination of activated T cells and Sendai virus. Nat Protoc, 2012. 7(4): p. 718-28. PubMed PMID: 22422317. ...

...generally validate our viral vector preparation protocol, and we are pleased to report that over 95% of...More about Virus Production Find Viral Vector Protocols ...

...PMC3775282. 2. McDevitt, Ross A., et al. "Serotonergic versus nonserotonergic dorsal raphe projection neurons: ...

...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

...Secreted Proteins: The most common purification protocols are designed for soluble, cystosolic protein products... can be recovered and purified using a special protocol. Alternatively, reducing the concentration of ...

...Kensaku Sakamoto 197099 pIYN3 TyrRS M. jannaschii halogenated tyrosines Bacterial TAG Kensaku Sakamoto 197100...Kensaku Sakamoto 197101 pCDF-Az TyrRS M. jannaschii azidophenylalanine Bacterial TAG Kensaku Sakamoto 197102...you are working off of a previously established protocol, make sure to match the growth medium, ncAA concentration... pMAH2-CageCys leucyl-tRNA-synthetase E. coli photocaged cysteine Mammalian TAG Huiwang Ai 73544 pEvol-pAcFRS...91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Bacterial TAG P....91706 pCMV-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Mammalian TAG P.... PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser...

...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1, 5 Deisseroth... 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive 8 Deisseroth... pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent...-Coff/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Flp dependent 8 Deisseroth...