We narrowed to 660 results for: otos

...engineers in your lab, you likely will need to write protocols, SOPs, and methods sections. The next time you...academic labs when creating shared experimental protocols. Clarify: Asking for clarification during a meeting...

.... Use the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn...for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip: Turn...

...has  developed a proprietary, low cost cloning protocol that he has used for cloning of more than 10,000...plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our molecular biology...

... RNA editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting...development and use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting...

... saves time and money when developing assays.  Protocol architecture The basic (very basic!) architecture...increased binding affinity, many indirect antibody protocols are designed to take place over multiple days....

...excitable. The mutants are co-expressed with a photoconvertible fluorescent protein mEOS4a. Cells containing...sensor that shows a favorable outcome are then photoconverted, sorted, and sequenced to identify the mutants...

...become slightly denatured during your experimental protocol, using a polyclonal antibody increases the likelihood...Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org/10.1101/pdb.top099101...

... with most multi-labeling immunohistochemical protocols where either a directly labeled primary or a primary...secondary antibody. Compatible with multi-labeling protocols when other antigens are detected with either a...

...FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning...delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is ...

... Their goal was to figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA... a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit the...

...to ABE7.10. Depending on the position in the protospacer, the editing capacity of the ABE8s ranged from...site in the genomic DNA to contain a specific protospacer adjacent motif (PAM) made up of 5′-NGG-3′ (“N...

...contact information, and potentially a photograph of yourself. A photo can be very useful in the event that...

...a group discussion on the best strategies and protocols for different experiments.  In this blog post...and resources page Browse our molecular biology protocols Read our molecular biology reference ...

...structures, etc., as well as the presence of a Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence...genome editing in stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI:10.1038/nprot.2016.171 ...

...encoded calcium indicators. Cold Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609. Palmer AE, ...genetically targetable fluorescent indicators. Nature protocols. 2006 Aug;1(3):1057-65. Resources on Addgene.org...

...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...with the IPD of a cation channel, such as the serotonin 3 receptor, will promote depolarization in neurons...

.... Recent studies have shown that the conditions Sotos-type 2 and Marshall Smith Syndrome are due to mutations...more accurate diagnosis of NFIX-related syndromes (Sotos-type 2 and Marshall-Smith) and could be expanded...

...fluorescence intensity due to interactions with photoactivatable fluorescent protein Dronpa. The authors tracked...peak of 519 nm. These proteins have improved photophysical properties and can be used in quenching, FRET...

...posts for updates on the most recent deposits, protocols, and tips for using retroviral vectors in your...of your choice. More information and a detailed protocol on how to use the pLKO.1 vector can be found here...

...anti-mouse antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody...sample. There are species-on-species staining protocols that use antibody fragments to block the endogenous...