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...are transcribed from the RNAP III promoters. This post features promoters for general RNAP II and RNAP ...constructs and we will discuss these in a future post on viral vector parts. Promoter specificity Aside...Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter...

...This post was contributed by Kartik Lakshmi Rallapalli, a graduate student at the University of California...preference than the ZFNs.   Genetic Engineering Era: Post CRISPR  CRISPR The strategy of using a protein to...the Genome Editing Trading Card seen in this blog post?Download high resolution versions here.  Many thanks...Additional resources on the Addgene blog Find more blog posts about CRISPR Learn more about the history of CRISPR...

...This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at...continue my scientific endeavors. I hope that this post can provide some perspective and advice to any undergraduate... you. Be proactive Rather than applying only to posted job offerings, the best research experiences typically... offer application fee waivers. One of the blog posts that helped me quite early on was Christine Liu’...graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting more scientific and professional...or 9-to-5 job. I had to ask to find out what a “postdoc” is, and that “PI” stood for principal investigator...admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar/student: visiting...

...sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated with a ...

...virus experiment. Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors...

...which contains the viral genome. In today’s blog post, we’ll focus on the AAV transfer plasmid and take...and combinations out there not discussed in this post, you’ve got the knowledge to be able to design a...WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary...

...) and subject age at time of injection. How long post-injection did you wait until performing the experiment...process of designing your experiment, read our blog post on important considerations when using AAVs. Experimental...

...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site ...publications. My classmates consisted of graduate students, post-grads, and early career scientists. Over the course...

...vector educational resources as well, with both posts on the blog and pages on the Addgene website. blog.addgene.org...virus. You can find all of our viral vector blog posts here. If you have virus expertise you’d like to ...

... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...Resources on the Addgene Blog Check out our blog posts on science communication Find interviews scientists...

...This post was contributed by guest blogger Meghan Monroy, a graduate student in Protein Science at the...on Your Plasmid Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for...

...using CRISPR/Cas9 RNPs? Check out this post Check Out Other Blog Posts on CRISPR Additional Resources on ...

...for our first post, I thought it best to define language you’ll see throughout the posts and introduce...field, often through manuscripts, seminars, or posters. It is important to note that it is the actual ...

...graduate student in the Roth lab, for editing the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin...blog Browse all of our fluorescent protein blog posts Find other fluorescent biosensors Resources on ...

...For more details on this technology, see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ...

...springs My husband, Paul Blainey, at that time a post doc in the Quake Lab, volunteered to do the sampling... other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another thermostable Cas9 Resources...was wonderful to learn that one project led by postdoc Dr. Stephanie Schmidt not only produced partially...

... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional Resources on Addgene.org Check Out ...

...communication skills. So, where can a graduate student or post-doc start? The good news is there are more opportunities...science on the one you already have. Read my earlier post about Scientists on Twitter here. 4)   If you enjoy...

... Plasmids 101 blog posts Learn more about the origin of replication Find blog posts about plasmid cloning...maintained in one bacterial cell. In this Plasmids 101 post, we’ll cover why this happens, how it might affect...

...stay up-to-date with these improvements. In this post, we discuss the sequencing technologies that Addgene...For more information on this process, see our blog post on NGS plasmid quality control. NGS is generally...process quickly while maintaining accuracy.  This post was written by Alyssa Shepard, with significant ...