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We narrowed to 755 results for: rest

Showing: 281 - 300 of 755 results

  1. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...plasmid with your gene of interest, you would replace mCherry with your gene of interest. pBLADEONLY: This plasmid...a pBAD33-based plasmid expressing your gene of interest, all you have to do is co-transform pBLADEONLY...pBLADEONLY. That way, a previously cloned gene of interest, under the pBAD promoter, can be controlled with light...

  2. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...well-equipped. Many institutions are sincerely interested in connecting with the public in a meaningful...scientific background and bring with them established interests and areas of expertise. Perhaps the strongest ... disparate spheres of research. In my case, an interest in neuroscience was carried over and allowed to... new intellectual environment. Over time, this interest came to incorporate other facets which occupied...

  3. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...available. Aguilar is a basic cell biologist. He got interested in Lowe Syndrome initially because of studies...disease associated with the gene, it piqued their interest. They obtained cells from patients with the condition...of the Wistar Institute, says that the lab got interested in XP out of a desire to understand the early...diseases at the same time. The limitation of budget restrained us from costly functional analyses.  “We believe...

  4. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...recapitulate the natural biology of a cell type of interest better than immortalized lines derived from the...it’s much more difficult to introduce a gene of interest into primary cells, so most primary cell lines...cell penetrating peptide (CPP) to a protein of interest thereby giving it the capacity to enter cells....29(1):33-41. PubMed. Protein transduction: unrestricted delivery into all cells? Schwarze SR, Hruska...

  5. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have a colleague...or other information that may be helpful or of interest to them. Don’t send a long email. If you have ...with people by discussing non-work topics and interests. Offer help when you can, instead of just asking... with people you don’t like or who do not show interest in you. Network with the people that you want ...

  6. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs of identical hairpins within one gRNA will...HaloTag with red fluorescent ligands to the loci of interest. Dual color detection In order to label two loci...sgRNA-Sirius-8XPP7) to recruit GFP to the loci of interest. To determine how the sgRNA-Sirius-8XPP7 and sgRNA-Sirius...that result in yellow fluorescence at the loci of interest, the team could visualize three loci and measure...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...standardized DNA constructs, but its use of Type IIS restriction enzymes results in scarless assembly. Finally...allowing fragments to be taken directly from restriction enzyme digests to DNA assembly. What makes it...containing regions of homology) cut from plasmids via restriction enzyme digestion, and used directly in DNA assembly...as easy as obtaining a plasmid with the gene of interest (search Addgene plasmids by gene here), designing...

  8. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...community by funding their travel to a conference of interest. In this post, we’re proud to announce our first...components - an enzyme that breaks down the HMO of interest into its component simple sugars (including lactose...controlled GFP. When the enzyme breaks down the HMO of interest, the lactose released activates expression of ...His-tagged ABC transporter known to bind to the HMO of interest. Fatima specifically proposes using this sensor...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...site of interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9...introduce diversification by targeting a site of interest with dCas9 fused to the Activation Induced Cytidine...nicking Cas9. A guide directs Cas9 to a site of interest, where it nicks its target DNA (left). Cas9 dissociates...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    ...with antibodies specific to your protein(s) of interest. Once the complex is captured, it is treated so...digestion can be appropriate when your protein of interest is a low abundance transcription factor or cofactor...point you have (1) crosslinked your proteins of interest to any DNA they were bound to (2) fragmented the...antibodies to specifically select for proteins of interest (4) isolated your antibody:protein:DNA complexes...

  11. The time and cost required to make a plasmid

    Type
    Blog Post
    ...plasmid, excluding any restriction enzymes or vector purchases (Table 1). Restriction enzymes and vector ...also a great way to get to know people doing interesting and relevant work in your field! However, there...work done previously, borrow small aliquots of restriction enzymes for one-off projects, or even save yourself...

  12. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...your cloning strategy: Restriction enzyme digestion Be mindful of the restriction enzymes you chose. Make...which technique you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden Gate etc.), ... fragments of the size you expect, check the restriction pattern of the enzymes you chose, and verify ...

  13. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...iterative one-pot reactions with straightforward restriction digestion and ligation steps to build barcoded...flexible and can accommodate any genetic elements of interest. It can thus be tailored to address the users’...effective gRNA sequences targeting your genes of interest. Thanks to the tremendous efforts made by various...the University of Hong Kong. He is particularly interested in developing and applying new technologies to...

  14. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...edition, we’re going to take a look at a very interesting tool that can be used for creating (excuse the...-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene expression in the absence of...described above, a genetic modification can be restricted based on experimental constraints. This has been...used to successfully introduce Cre into cells of interest. What if I need two separate recombination events...

  15. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...vector file, and quickly clone their vector of interest. And it truly is plug-and-play. Because of its...recommend checking your cloning via a miniprep and restriction digest, and then plasmid sequencing if you can...Cloning specifics Fragmid uses Bbsl, a Type II restriction enzyme that cleaves outside of its recognition..., 93% had the correct banding pattern in the restriction enzyme digest. They sent 82 of the correctly ...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...since 2015 to conditionally degrade proteins of interest in cells (Zhang et al., 2015). Recently the Ward...degron (AID) sequence attached to your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 ...) tagged proteins. Degradation of your protein interest would also result in depletion of the FP, which...integrate the tagged AID* into genomic sites of interests via CRISPR techniques. This tool-kit includes ...

  17. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...promoters could be an easier way to restrict expression of a gene interest in an animal model. A toolkit ...Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular cell types...types, pairs of synthetic promoter can be used to restrict expression to a single cell type based on the ...

  18. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...finding journal articles related to your actual interests while also staying on top of the ever expanding...which is a nice way to see growth or decline of interest in a field over time. Creating an alert for a ...places to look for more articles on your topic of interest.  Best uses Accessing the vast body of biomedical...keywords, concepts, journals, and/or papers of interest. You can also just follow feeds curated by Meta...

  19. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...transduced cells and loss of transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes... in trans. The Gutless AdV have generated high interest for gene therapy due their increased cloning capacity...plasmids: a shuttle vector (in which the transgene of interest is cloned) and pAdEasy™ which contains the adenoviral...plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm its integrity...

  20. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...inserting a targeting vector containing the gene of interest into a zygote, or into embryonic stem cells that...gene construct can be used to insert the gene of interest into a zygote or into embryonic stem cells. Non-homologous...genome using homology arms that flank the gene of interest. In either case, a positive selection marker such...the genome with similar homology to the site of interest or create mosaic animals where not all cells in...
Showing: 281 - 300 of 755 results