We narrowed to 526 results for: sequence

... universal sgRNA that targets the donor vector sequence. Homology arms that flank the donor plasmid cargo...essential genes and phage host factors, and identify sequence-specific toxicity of Cas9. Read the paper in ...

...exploration into the effects of ancestry and germline sequence variation in cancer cell lines took them down ...indicating loci where SNPs reside on sgRNA target sequences for sgRNAs that target ancestry-associated genes...frequency (x-axis) which SNPs map to thetargeting sequences of guides across 8 CRISPR libraries. Samples are...

...PCR is most commonly used in molecular cloning, sequencing, and genotyping. It’s extremely useful but is...such as in colony PCR, or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase...replicates. ddPCR can be used to quantify DNA sequences that are rare, for example, rare alleles or mutations...

... capacity AdV) are devoid of all viral coding sequences and retain only the ITRs and packaging signal ...analyzed by endonuclease restriction digest or fully sequenced to confirm its integrity. Initial production (... quantity and primers specific for a viral DNA sequence. *Pro-Tip*: Ad5 reference material should be used...

...The gRNA conventionally contains a 20-nucleotide sequence that is complementary to the target site, or protospacer...several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.     References ...to Our Interview with Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators...

...CRISPRi cassette flanked by left and right Tn7 end sequences. Donor bacteria with a chromosomal copy of the...recipient bacteria due to site selection by TnsD, a sequence-specific DNA binding protein. Selection removes...DNA (purple) flanked by left and right Tn7 end sequences (green) integrates downstream of the glmS gene...

... repeating elements anymore. The kit contains sequences for several different fluorescent proteins. Visualizing... of work on wheat rusts and we are collecting sequences that will provide a similar resource for those...they aren’t sure how to convert their own coding sequence to the MoClo system. It’s quite straightforward...

...plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors...

...pH can denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds...backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination. In depurination...

...scores provided by authors rather than sgRNA-level sequence read scores. The original supplemental data tables...query screen data without the need to analyze raw sequence data.  This format is inherently flexible and ...

...addition to peer review than having your plasmids sequenced and openly registered for other scientists to ...we won’t distribute materials with unresolved sequencing issues or if you’ve asked to cancel the deposit...

...series. Single DSBs resolved by NHEJ at coding sequences often result in loss-of-function frameshift mutations...analysis, allele-specific PCR, T7EN1 cleavage assay, sequencing, RT-qPCR, or immunoblotting to detect. Another...

...targets 18,080 human genes with 64,751 unique guide sequences to enable both negative and positive selection...proof-of-principle that CRISPR-Cas9 can be used to target any sequence in the genome. Tips and tricks Wang offers this...

...Pennsylvania independently searched for this unique sequence of DNA and both identified lymphocyte antigen ...as a receptor for PHP.B by looking for a unique sequence of DNA that was present in mice whose CNS was ...alleles that was driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to...

...the cap plasmid library by inserting short DNA sequences that contain all possible combinations into a ...plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid pool ... animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues...

...ten base pairs in length. An emerging family of sequencing techniques function as a kind of “GPS for the...over time, we periodically use next-generation sequencing to measure the relative proportion of guide RNAs...insulators, and more. I then identified valid gRNA sequences without off-target effects for the 2+ million ...

...protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and...

...antibodies will come with access to the antibody sequences, in line with the rest of Addgene’s recombinant...

... predefined DNA sequence that uses trprimers and enzymes that will amplify a sequence only if present....

...Rosella. When fused with a mitochondria-targeting sequence, Rosella can be used to track the targeted autophagy..., but the addition of a mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It...