Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 538 results for: sequence

Showing: 281 - 300 of 538 results

  1. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...targeting system could be used to routinely deliver sequences of interest to cells with efficiencies of 1-40%...CRISPR is remarkably effective for introducing non-sequence specific frameshift mutations through aberrant...template. It is this application - the creation of sequence-specific knockins - for which the AAV approach...currently focuses on determining the mechanisms and consequences of cohesin gene inactivation in human cancer...

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ... however, Cas9 allows you to easily change the sequence targeted by the activator by simply providing ... consist of plasmids that over-express coding sequences from a given cell type or organism, have been ...several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.     References ...

  3. TALEN Guide

    Type
    Collection
    ...other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists have identified a large...incentives are high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers...Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating mammalian...

  4. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Mismatches between the guide RNA and the target sequence lower the repression level. By controlling the...motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP concentration...

  6. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...host machinery to make additional copies. ori sequences are generally high in As and Ts. Why, you ask?...that affect plasmid copy number  Although the sequence and regulation of the ori dramatically affect ...

  10. Cultivating Community Science at BosLab

    Type
    Blog Post
    .... For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments... to collect various soil samples, extract DNA, sequence it, and analyze the data to characterize the microbiomes...

  12. An Addgene Summer Internship

    Type
    Blog Post
    ...discussion, we sent it for sequencing. The gene was successfully sequenced, confirming that we had made...

  13. Zebrafish Plasmid Collection

    Type
    Collection
    ... for plasmids that contain a zebrafish gene or sequence . The table below highlights plasmids that contain...Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish Model...zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...of target sequence in the 3' UTR David Bartel 12179 pIS1 Renilla Insertion of target sequence in the 3'... Bartel 12177 pIS2 Renilla Insertion of target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla... PCD FL0X Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly...psi-CHECK3 Firefly, Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly luciferase...

  15. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

  16. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... the following reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer...Our final step of the QC process, viral genome sequence verification, may not yet have been completed ...

  17. CRISPR Plasmids - Plants

    Type
    Collection
    ...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...to target an ssRNA of interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes...recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules...SHERLOCK. Cas12a is targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome...

  19. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...amino acid sequence required for BirA recognition and biotinylation. This 13 amino acid sequence is termed...Biotin can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using...

  20. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ... Both the plasmid (blue, backbone) and the DNA sequence of interest (green, insert) are cut with restriction...in the direction you want - usually by Sanger sequencing or further restriction digests. Of course there...appropriately sized insert, you should send it for sanger sequencing using primers that will allow you to read over...
Showing: 281 - 300 of 538 results