...often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, fluorescence-based...method is often used preceding next-generation sequencing or microarray studies and is not used as much...

...discussion, we sent it for sequencing. The gene was successfully sequenced, confirming that we had made...

.... For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments... to collect various soil samples, extract DNA, sequence it, and analyze the data to characterize the microbiomes...

...downstream measurement of miRNA efficiency on a target sequence. The gene of interest is cloned into a plasmid...luciferase, until miRNA target and degrade the mRNA sequence, resulting in a decrease in luciferase activity...

...immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular gRNA. In the enChIP ...For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding ...

...to target an ssRNA of interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes...recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules...SHERLOCK. Cas12a is targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome...

...amino acid sequence required for BirA recognition and biotinylation. This 13 amino acid sequence is termed...Biotin can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using...

... Both the plasmid (blue, backbone) and the DNA sequence of interest (green, insert) are cut with restriction...in the direction you want - usually by Sanger sequencing or further restriction digests. Of course there...appropriately sized insert, you should send it for sanger sequencing using primers that will allow you to read over...

... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...

... of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA assembly available...experimental and primer design based on fragment sequences and the polymerase used in the amplification. ...

...eukaryote (S. cerevisiae) to have its genome fully sequenced There are several human disease models established...Genome manipulation K. phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for...

...behavior, many many images of brain regions, or sequences of microbial DNA they all seem to require some...biological tools. In addition to the researcher using sequencing technologies to study microbiome interactions...

...produced by all three methods are identical in sequence. TAL effector repeat arrays produced on this framework... ZiFiT Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional...

...of a 19 nucleotide tetracycline operator (tetO) sequence, and is recognized by the tetracycline repressor... the tetR portion of tTA will bind these tetO sequences and the activation domain promotes expression.... tetR. This precludes tTA binding to the tetO sequences and subsequent increase in expression by the activation... EF1α promoter driving tTA; contains insulator sequence; plasmid pEnt L1L3 rtTA-3 is a rtTA version tTA...

...Mismatches between the guide RNA and the target sequence lower the repression level. By controlling the...motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP concentration...

...other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists have identified a large...incentives are high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers...Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating mammalian...

...used by scientists to introduce different DNA sequences of interest into a bacterial cell or a host’s ...direct contact. The donor bacterium contains a DNA sequence called the Fertility factor (F-factor). The F-...

...scaffold and cloning sites for inserting the guide sequence (pSpCas9(BB)). The 2A-GFP allows for screening... plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1...

...are necessary for recognition and cleavage. This sequence flexibility indicates that gRNAs could be incorporated...tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage. gRNAs...

...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn...