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Showing: 281 - 300 of 602 results
  1. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time...proteins for your imaging needs. If you need an FP refresher, see our FP blog posts.     References Sattarzadeh...
  2. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ... of Human Life at the MOS. (If you want a fun refresher on the Central Dogma of Biology, I would stop ...cocktail hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as ...
  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...
  4. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources Further reading Wang, J. Y....Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet  Resources on the Addgene blog A Needle in a Base-Stack...
  5. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t ...While all viruses have a genome inside a protein shell known as a capsid, some also have a lipid bilayer...
  6. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor...
  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is exceptionally ...
  8. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...and maintenance of good representation. Addgene wishes you luck in your future library use and screening...
  9. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...do just this.  In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...tracking their fulfillment manually in a shared spreadsheet and it takes time away from our research. For...
  10. Molecular Biology Reference

    Type
    Guide
    ...The insert is typically the genetic element one wishes to study using a particular plasmid. Promoter Region...expression of an endogenous gene. This is frequently accomplished through expression of an shRNA targeting the...edit genomes. Genome editing is most commonly accomplished using CRISPR technology. CRISPR is composed ...concentrations. We suggest checking your plasmid's datasheet or the plasmid map to confirm which antibiotic...with a different fluorophore so they can be distinguished from one another. Similar to DNA replication...
Showing: 281 - 300 of 602 results