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TypeBlog Post...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time...proteins for your imaging needs. If you need an FP refresher, see our FP blog posts. References Sattarzadeh...
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Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post... of Human Life at the MOS. (If you want a fun refresher on the Central Dogma of Biology, I would stop ...cocktail hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as ... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...irreversible protein-peptide interactions. In a study published in Proceedings of the National Academy of Sciences...Proceedings of the National Academy of Sciences paper [published online on March 17] should cover it. Addgene: ... -
Build Your CRISPR Vocabulary
TypeBlog Post... to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources Further reading Wang, J. Y....Addgene's CRISPR 101 eBook Addgene's CRISPR Cheat Sheet Resources on the Addgene blog A Needle in a Base-Stack... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps capture... your own lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...video of her singing about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich...UriAlonWeizmann, here my song about our paper recently published in @nchembio Title: Bye bye L-arabinose drive.... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t ...While all viruses have a genome inside a protein shell known as a capsid, some also have a lipid bilayer... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...serotype of our AAV preparations. We recently published a description of the VGS process and use cases...doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer A, Mevel M, ... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog Post... in synthetic biology and DNA synthesis. They published their results in a paper in Nature Communications... Nielsen & Christopher A. Voigt also recently published a tool (trained on Addgene plasmid datasets) to... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...using the power of light. In a recent article published in Nature Methods, Boyden and his team describe...can figure out what they are necessary for. We published our first paper on optogenetic tools for neuroscience... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post...made to moderate and cross-check all data with published literature as soon as possible after submission... queries. Looking for a bright, monomeric FP published in the last few years with an emission peak around... -
K. phaffii: Rising to the Occasion
TypeBlog Post...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is exceptionally ... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post...collaborative cloud tools such as Google Docs and Sheets during Zoom lab meetings to chip in ideas and take...collaborative writing and this recent article published in PLOS Computational Biology (Frassl et al., ... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...and maintenance of good representation. Addgene wishes you luck in your future library use and screening... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...do just this. In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...tracking their fulfillment manually in a shared spreadsheet and it takes time away from our research. For... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...Additionally, proper citation of these Addgene IDs in published work allows readers to quickly track down the ...Resource Identifiers (CURIEs) combined with well-established prefix resolution services such as Identifiers.org... -
Molecular Biology Reference
TypeGuide...The insert is typically the genetic element one wishes to study using a particular plasmid. Promoter Region...expression of an endogenous gene. This is frequently accomplished through expression of an shRNA targeting the...edit genomes. Genome editing is most commonly accomplished using CRISPR technology. CRISPR is composed ...concentrations. We suggest checking your plasmid's datasheet or the plasmid map to confirm which antibiotic...with a different fluorophore so they can be distinguished from one another. Similar to DNA replication... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...imaging Improving Well-established High-throughput assays Available Well-established Natural and engineered...