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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 727 results for: SON

Showing: 151 - 160 of 727 results

  1. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...PMC4246457. 4. Davidson CJ., et al. "Improving the limit of detection for Sanger sequencing: a comparison of methodologies...although this has been improved in some studies) (Davidson et al. 2012, Tsiatis et al. 2010). As the field... approaches can provide the researcher with a reasonable level of certainly that the effects they see ... PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute at the University of Cambridge...Vouillot, Léna, Aurore Thélie, and Nicolas Pollet. "Comparison of T7E1 and surveyor mismatch cleavage assays...PubMed Central. 5. Tsiatis, Athanasios C., et al. "Comparison of Sanger sequencing, pyrosequencing, and melting...

  2. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...such a resource when updates depend on a single person. With thousands of scientists routinely using and...Protein Property Visualization allows for visual comparison of FPs and how they relate to one another. .  ...you or your lab use frequently) for the sake of comparison or ease of retrieval.  These collection pages...of your choosing!), and they can be downloaded in JSON or CSV format to quickly retrieve all of the information...store and represent within-study photobleaching comparisons.  We very much appreciate feedback, so try out...plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein Collection ...

  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Non-science favorites  The Mistborn series by Brandon Sanderson "The Mistborn series is the first 3 books of a...funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer Sarum: The Novel of England...skeptical reader of historical fiction (I can personally attest to this!). Following history from the ...participated in a book club before, but I had a few reasons for launching this one. On a purely selfish level...contentious, discussions. I presented the first book, Nicholson Baker’s The Anthologist. The book ended up literally...Prejudice, and some more obscure treasures, like Tove Jansson’s The True Deceiver. There have been books that ...

  4. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...be a graduate student, postdoc, professor, etc. Reasons to pursue a visiting role The main motivation to...can’t obtain at your current institute. A common reason is hands-on training in a technique that only a...hard to find, or inaccessible to you for another reason. Visiting would allow you to train and acquire ...data you need on the new instrumentation. Bonus reasons Visiting gives you access to possible new collaborations... willing to host you! If there is someone you personally know or your lab collaborates with that can fulfill...work together to ensure your expectations are reasonable, your reagents are onsite before you need them...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ... Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB (2017) A stable but reversible integrated...assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA in 2017 to enrich and identify...Wen et al., 2018). SRIRACCHA uses the Piggybac transposon to stably integrate a reporter gene that contains...your cells.  A plasmid that contains Cas9. The Madison lab also created an inducible SRIRACCHA system ... experiment that targeted the gene NFE2L2, the Madison lab found that H2B-GFP expressing cells exhibited...

  6. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...If cloning methods had personalities, SLIC (sequence- and ligation-independent cloning) would be a true...also be beneficial. SLIC is most often compared to Gibson assembly, a cloning method also based on homologous... recombination. The higher temperature at which Gibson assembly takes place may limit formation of secondary... of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase is much less...less expensive than the enzymes required for Gibson assembly. SLIC is a standardized method for multi-fragment... Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning...

  7. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ... think more favorably of scientists they know personally Scientists in your field will know about grants...Job hunting outside of academia is all driven by personal connections What can you do to get the most from...whose company they enjoy on a professional and personal level.  Here are some tips to get out of the rut...free places to order cards. There are a few good reasons to have a card but two of the best are: if you ...with a more general intro until you know if the person you are talking to is really in your direct area...attend. Meet some people online to follow up with in person. Email lab members that have moved on to see if...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... https://doi.org/10.1021/acssynbio.6b00031. Iverson, Sonya V., et al. “CIDAR MoClo: Improved MoClo Assembly...proteins, then the CIDAR Kit is an excellent option (Iverson et al., 2016). CIDAR offers a one-pot reaction ...developers noticed that studies frequently made comparisons between CRISPR components expressed from different...nature of the Fragmid system allows for direct comparisons of CRISPR parts. The Fragmid system was used ...fungi, bacteria, protists, plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters...Crossref, https://doi.org/10.1016/j.xgen.2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden...

  9. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that happens...Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn is a place to... to build relationships. If you don’t add a personal note, you waste an opportunity to start things out...Connect.” If you click this box, it will send an unpersonalized, generic invitation. Don’t try to connect from...have had success with this and as long as you personalize the invitation with your intention for connecting...

  10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...CRISPR-Cas systems.   Four years later, the Sontheimer and Davidson labs discovered three anti-CRISPRs for ...Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA (2017) A Broad-Spectrum...Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR (2016) Naturally Occurring Off-Switches...
Showing: 151 - 160 of 727 results