We narrowed to 643 results for: ato

1. With Our New Viral Service, We're Taking CRISPR Further!

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   Blog Post

   ... your gene of interest with a variety of Cas9 activators available in lentiviral format. Finally, while...

2. Networking Follow-up: I Met a Person. Now What?

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   ...ask my benchmate to take my plates out of the incubator because I’d had a baby that night, all he said...

3. What Good Citizenship Can Do for Reproducibility in Science

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   ...filtration system, environmental stability in an incubator), but there’s a lot you can do to help other scientists...

4. Plasmids 101: NGS Quality Control for Pooled Libraries

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   ...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...

5. Four Factors that Differentiate the Stem Cell Field

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   ...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...

6. Addgene Welcomes John Doench to the Advisory Board

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   ...targets and what confers specificity in the miRNA regulatory process. This early research is still relevant...

7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   ...received his PhD in biophysics from UCSF in the laboratory of Ronald Vale, after which he was a fellow at...

8. Finding and Joining Your Dream Lab

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   ...when seeking your dream lab. The principal investigator's point of view If you are looking to join a ...

9. CRISPR History and Development for Genome Engineering

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   ...Activate or Repress : dCas9 fused to transcriptional activators or repressors can modulate transcription in a...

10. Behind the scenes: Addgene’s new search engine and more

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    ...of other websites, we built our new search engine atop Elasticsearch, a powerful open-source search engine...

11. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...microRNAs (miRNAs) are recognized as important regulators of post-transcriptional gene expression across...

12. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    ...and leveraging tools like the Myers-Briggs Type Indicator or DISC assessments can help you navigate team...

13. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    ..., the stem-loop structure of transcriptional terminators. Another potential issue is sequence similarity...

14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression...

15. Communicating Your Science With Help From ComSciCon

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    ...in all STEM fields become excellent science communicators. To empower graduate students to become ambassadors...

16. Why and how to start studying chromatin (with Addgene's help)

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    ...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...

17. Building and Validating Recombinant Antibodies: A Case Study

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    ...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

18. Antibodies 101: Monoclonal Antibodies

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    ...the antigen-binding sites of the antibody - the paratopes - may change and impact the specificity and avidity...

19. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    ...been used in yeast by Lee et al (2013) and in nematodes by El Mouridi et al (2017) and Heppert et al (...

20. Multiplex Genome Editing with CRISPR-Cpf1

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    ...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple...

21. Top Requested Lentivirus and AAV of 2016

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    ..., Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible ...

22. Sharing is Caring: But How to Distribute Open Hardware?

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    ... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for...

23. Plasmids 101: Screens vs. Selections

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    ...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

24. How to Make Friends and Meet People at a Scientific Conference

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    ...You never know when you will meet a potential collaborator Some of these people you meet will review your...

25. Dos & Don'ts When Publishing a Scientific Manuscript

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    ...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...

26. Bacterial Expression Systems

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    ...Two-Hybrid System Various Plasmids placO with lambda-C1 operator Beta-galactosidase Ann Hochschild Bacterial two-hybrid...

27. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    ...makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons, the channelrhodopsins...

28. RNA Extraction Without A Kit

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    ...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...

29. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...and were presented at the ASPB meeting by the collaborators who could participate. Despite the group not...

30. Addgene: The First Twenty Years

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    ...amazing years in front of us. So in our last celebratory moment, we wanted to walk through some of our...