Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 604 results for: abo.2

Showing: 601 - 604 of 604 results

  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....learn more about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design...interest, I encountered low efficiency (0.13%) and laborious handling (screening 768 F1 animals). Recovering...

  2. Protocol - How to Design Primers

    Type
    Protocol
    ...-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs Melting temperature (Tm) of 50-60°C Primer...amplification process. When designing, if unsure about what nucleotide to put at a certain position within...capabilities. Taking into consideration the information above, primers should generally have the following properties...

  3. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...DNA concentration alone. One method is to conduct 2 ligations for each plasmid you are trying to create...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...window) New England Biolabs for more information about restriction enzyme buffers). If you select enzymes...

  4. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...DNA concentration alone. One method is to conduct 2 ligations for each plasmid you are trying to create...Background In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same ...
Showing: 601 - 604 of 604 results