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We narrowed to 929 results for: Comp;

Showing: 751 - 800 of 929 results

  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...or producer cells for further processing. What component(s) you need to collect will depend on the serotype...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...protein fusion. CRISPR genome editing can help accomplish this goal. The power of this system lies in the...PMID: 27052166. Kunzelmann, Stefan, et al. “A Comprehensive Toolbox for Genome Editing in Cultured Drosophila...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology...time. Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of...

  6. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... or very similar buffers if not, since buffer components can affect the final readout. When preparing ...using advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind...

  7. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

  8. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

  9. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...gauged interest from relevant parties, and the company at large, by polling and informal communications...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    ...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

  11. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s...particular cell type, or a subset based on a cellular compartment or growth stage). A prey library can either be...

  14. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...

  15. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...roughly measures granularity, a proxy for nucleic complexity of the cell.  The sample will have been labeled...labeled using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/...

  16. Viral Production at Addgene

    Type
    Blog Post
    ...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

  18. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...varied codon preferences among organisms aren’t completely understood, but some possible reasons include...codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization...

  19. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...is a terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer...

  20. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st, according...centrally administered, which leads to many complications, including the inability of some institutions...

  21. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...al. outline a do it yourself PCR-based approach complete with freely available positive controls. Routinely...

  23. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live...which yields a much weaker signal on live cells compared to dead cells. As a result, the largely negative...

  24. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal ...size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity...

  25. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...undetectable in the blood. But the virus doesn’t completely disappear; it just hides in latently infected...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...

  26. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...increased gene activation at the targeted site compared to other Tet1 delivery systems. Transcriptional...

  27. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... domain structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG...5). Figure 5: TurboCas protein labeling compared to immunoprecipitation. The top panel depicts ...

  28. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors ..., K. L., Ayasoufi, K., & Barry, M. A. (2019). Comparison of Gene Delivery to the Kidney by Adenovirus,...

  30. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation...

  34. New Coomassie Protocol Video

    Type
    Blog Post
    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

  35. New Western Blot Protocol Video!

    Type
    Blog Post
    ...That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi run a...

  38. CRISPR Guide Update

    Type
    Blog Post
    ...well as alternatives to Cas9. All of this is accompanied by new or refreshed figures made using BioRender.com...

  39. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...updating our CRISPR guide. Our CRISPR guide is a comprehensive resource that covers most things CRISPR - from...

  40. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... gave to me: efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth...
Showing: 751 - 800 of 929 results