We narrowed to 911 results for: Tro;

...bright, monomeric green fluorescent protein. In vitro, mWasabi is 1.6-fold brighter than EGFP and is photostable...

...Educational Resources The Basics of Western Blotting Introduction to Immunofluorescence Addgene’s Antibody Guide...

...do quality science on interesting topics Having strong non-science skills is important for success Must...

...of Biologically Functional Bacterial Plasmids In Vitro.” Proceedings of the National Academy of Sciences...

... began by isolating a calcium-binding protein, troponin C (TnC), from muscle cells. They identified a ...

... morning, but the temptation to skip the gym is strong. There are a few women that I see at the gym every...

... which will allow researchers to independently control the activity of two populations of neurons at once...

...spread across literature in publications that introduce these tools. Years ago, Kurt Thorn (UCSF at ...

...Tsang OT-Y, Chan K-H, Yuen K-Y (2020) Comparative tropism, replication kinetics, and cell damage profiling...

...section of a manuscript they might be found, i.e. introduction, methods, results; or phrases are categorized...

...- Marcy Patrick, Associate Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke...

... mediated by a conserved cysteine.  With the introduction of MerMAIDs, neurons can be turned off quickly...

...The button is on the right of the Share Screen control panel on the top of the screen and every participant...

...  Susanna Stroik...

...bacteria. In a gram negative bacteria, the alcohol destroys the outer lipid membrane, and the thin inner peptidoglycan...

... multiple genetic diseases, such as muscular dystrophies, heart disease, and type 2 diabetes, across four...

...photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while having...

...researchers explore the complexity of translational control in cellular physiology. Ribosome profiling Ribosome...

...good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel, PowerPoint...

... could be used in clinical trials. The quality control process alone takes nearly a year. Addgene depositor...

...resource to help me. I’m still waiting for a mass spectrometry app. I digress. When I started learning proper...

...with Dr. Doench for an interview to help further introduce him to the Addgene community. Listen to the interview...

...effective delegation skills: The team will be stronger and less dependent on the manager to succeed. ...

...background on fluorescence and filter sets, see the Introduction to Fluorescence Microscopy lecture at iBiology...

...genome, and showed success in models of muscular dystrophy. In this case, Konermann et al. targeted MAPT,...

...industrial operation would ever consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation...

...individually (~20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing...

...more about research applications for antibodies in Intro to Antibodies Read about the Developmental Studies...

...more open way of communicating in science brings stronger networking across laboratory and disciplinary ...

...They might be researching enhancer elements that control the transcription of a gene a megabase away, or...

...both small and potentially less likely to elicit a strong immune response, factors that may make it especially...

...-Cas9 tools have been used in immunology for in vitro screens of immune cell activity. However, a CRISPR-based...

...NanoLuc® Luciferase Fluorescent Proteins 101: Introduction to FRET Resources on Addgene.org Luciferase...

...  Susanna Stroik...

...  Susanna Stroik...

... Susanna Stroik...

...wide expression, with no liver expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen...

...Because the performance of a fluorescent protein strongly depends on both the biological system as well ...

...probes, with sufficient signal for in vivo and in vitro imaging. Saito et al. used this Nano-lantern to ...

...difficult to accurately call a file. Version control your commonly used scripts: If you edit a general...

...equipment that still works. “We use functional spectrometers from the early 1990s that were free and free...

...protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic...

...in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged...

...create a barcode for your plate, you will get in trouble if you don’t qualify what kind of “plate” you mean...

...practices for reproducibility: data management plans, electronic lab notebooks, wet lab protocols, reagent sharing...

... audience.  More resources on the Addgene blog Intro to our Summer SciComm Series...

...individually keep track of any failures and do troubleshooting on their own. For a few samples this isn’t ...

...Created with BioRender.com. To decrease the electropositivity of the HNH/RuvC groove, Slaymaker et al. made...

...similar to an academic research environment” with a strong focus on publishing, but still made it so she could...

...allow it. Before you start your presentation, introduce yourself to your audience. Pay attention to...