We narrowed to 916 results for: Tro;

1. Sleeping Beauty Awakens for Genome Engineering

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   ...laboratory setting, transposons can be used to both introduce genes into an organism’s genome (see figure) and...

2. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ...Addgene was mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely...

3. 10 Ways to Share Your Science!

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   ... distant island. He got a little money for his trouble, too. 7)   Reach out to science writers in your...

4. Advice for Choosing a Research Project

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   ...by your own subject - maybe something used in astrophysics can be applied to fungal biology.” Connie Cepko...

5. The Stingy Scientist: How the Baby Gel Box Was Born

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   ... your fingers in during a run or risk minor electrocution.  These days you can buy small gel boxes, but...

6. Plasmids 101: Antibiotic Resistance Genes

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   ...disrupt genes at the chromosomal level. Scientists introduce an antibiotic resistance cassette within the coding...

7. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   ...bright, monomeric green fluorescent protein. In vitro, mWasabi is 1.6-fold brighter than EGFP and is photostable...

8. Addgene Begins Distribution of Recombinant Antibodies

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   ...Educational Resources The Basics of Western Blotting Introduction to Immunofluorescence Addgene’s Antibody Guide...

9. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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   ...current antibody plasmid collection) and we plan to introduce affinity reagents in protein format within the...

10. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    ...visit places not accessible to the public and retrofitting our car with a freezer wired to a charger and...

11. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    ...do quality science on interesting topics Having strong non-science skills is important for success Must...

12. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    ...of Biologically Functional Bacterial Plasmids In Vitro.” Proceedings of the National Academy of Sciences...

13. Hot Plasmids: FRET-Based Biosensors

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    ... began by isolating a calcium-binding protein, troponin C (TnC), from muscle cells. They identified a ...

14. Mentoring for Scientists: I Have a Mentor, What Now?

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    ... morning, but the temptation to skip the gym is strong. There are a few women that I see at the gym every...

15. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    ... which will allow researchers to independently control the activity of two populations of neurons at once...

16. Trading Spaces - Visiting Scientist Edition

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    ...  Susanna Stroik...

17. Reagent Repositories Are Speeding up Science During the Pandemic

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    ...Tsang OT-Y, Chan K-H, Yuen K-Y (2020) Comparative tropism, replication kinetics, and cell damage profiling...

18. Celebrate National Book Month with Addgene

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    ...- Marcy Patrick, Associate Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke...

19. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ... mediated by a conserved cysteine.  With the introduction of MerMAIDs, neurons can be turned off quickly...

20. Early Career Researcher Toolbox: Free Tools for Science Writing

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    ...section of a manuscript they might be found, i.e. introduction, methods, results; or phrases are categorized...

21. Revamp Your Lab Meetings With Creative Virtual Collaboration

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    ...The button is on the right of the Share Screen control panel on the top of the screen and every participant...

22. Plasmids 101: Gram Negative and Positive Bacteria

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    ...bacteria. In a gram negative bacteria, the alcohol destroys the outer lipid membrane, and the thin inner peptidoglycan...

23. DAP arrays for multiplex gene editing

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    ... multiple genetic diseases, such as muscular dystrophies, heart disease, and type 2 diabetes, across four...

24. FPbase: A new community-editable fluorescent protein database

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    ...spread across literature in publications that introduce these tools. Years ago, Kurt Thorn (UCSF at ...

25. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    ...photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while having...

26. Visualizing Translation at the Single Molecule Level

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    ...researchers explore the complexity of translational control in cellular physiology. Ribosome profiling Ribosome...

27. Science Career Options

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    ...good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel, PowerPoint...

28. Four Factors that Differentiate the Stem Cell Field

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    ... could be used in clinical trials. The quality control process alone takes nearly a year. Addgene depositor...

29. 5 Great Apps for Lab Life

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    ...resource to help me. I’m still waiting for a mass spectrometry app. I digress. When I started learning proper...

30. Addgene Welcomes John Doench to the Advisory Board

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    ...with Dr. Doench for an interview to help further introduce him to the Addgene community. Listen to the interview...

31. Management for Scientists: Delegating is Key

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    ...effective delegation skills: The team will be stronger and less dependent on the manager to succeed. ...

32. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...background on fluorescence and filter sets, see the Introduction to Fluorescence Microscopy lecture at iBiology...

33. Fluorescent Proteins 101: Luciferase

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    ...luciferase gene in the plasmid, which is then introduced into the desired mammalian, plant or bacterial...

34. CasPEDIA: A Functional Classification of Cas Enzymes

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    ...  Susanna Stroik...

35. Hot Plasmids - May 2022

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    ...wide expression, with no liver expression, after retro-orbital sinus vein injection of a CAG-driven mNeonGreen...

36. Hot Plasmids - February 2022

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    ...individually (~20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing...

37. Antibodies 101: Monoclonal Antibodies

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    ...more about research applications for antibodies in Intro to Antibodies Read about the Developmental Studies...

38. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...-Cas9 tools have been used in immunology for in vitro screens of immune cell activity. However, a CRISPR-based...

39. Sharing is Caring: But How to Distribute Open Hardware?

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    ...more open way of communicating in science brings stronger networking across laboratory and disciplinary ...

40. How Addgene Creates Content

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    ...  Susanna Stroik...

41. Plasmids 101: Screens vs. Selections

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    ... Susanna Stroik...

42. Developing a cold tolerant E. coli using specialized chaperones

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    ...industrial operation would ever consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation...

43. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...both small and potentially less likely to elicit a strong immune response, factors that may make it especially...

44. Why and how to start studying chromatin (with Addgene's help)

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    ...They might be researching enhancer elements that control the transcription of a gene a megabase away, or...

45. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    ...genome, and showed success in models of muscular dystrophy. In this case, Konermann et al. targeted MAPT,...

46. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    ...Because the performance of a fluorescent protein strongly depends on both the biological system as well ...

47. Luminescent Imaging with Nano-lanterns

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    ...probes, with sufficient signal for in vivo and in vitro imaging. Saito et al. used this Nano-lantern to ...

48. How to Keep a Lab Notebook for Bioinformatic Analyses

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    ...difficult to accurately call a file. Version control your commonly used scripts: If you edit a general...

49. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    ...equipment that still works. “We use functional spectrometers from the early 1990s that were free and free...

50. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...practices for reproducibility: data management plans, electronic lab notebooks, wet lab protocols, reagent sharing...