Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,025 results for: URE

Showing: 751 - 800 of 1025 results

  1. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...stressors, including emotional stress or changes in temperature.  “The disease was first described in 1971,” ..., it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012, researchers identified the ...Thailand will receive much better health care in the future based on our studies.” Thank you to all the BeHeard...

  2. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...that we aren’t still pitching for feedback.  Your venture should continuously evolve and respond to feedback...number of experts, including Harvard/MIT faculty, venture capital and angel investors, and business development...idea has potential to make a difference, then make sure you’re able to answer every possible question related...

  3. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...addition to thorough ethical considerations, in making sure that the research has been adequately proven to ...unethical behavior (it’s hard to argue otherwise). To be sure, throughout the history of medical research, there...on necessary actions to review and decide on the future of genome editing technology. We must stop punting...

  5. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...that are then barcoded for identification. This mixture of barcoded fragments is known as a *library*. ...additional benefit of plexWell™’s sequential tagging procedure, the quantities of library molecules for each ...multiplexed pool. The cost of these normalization steps, measured in time and consumables, can be daunting for large...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...model organism for a few reasons: Affordable to culture and easy to work with Grows extremely fast (doubling...induced by removing methanol from the media. This feature makes the strain tractable for peroxisomal studies... for genome engineering. A unique evolutionary feature of K. phaffii, relative to S. cerevisiae, is that...

  7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...receiving only 1 TF expression construct. Cells were cultured following a differentiation protocol before performing...so that a single TF was delivered to each cell, future applications could deliver multiple TFs to each...on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction...

  8. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... the library, use next-generation sequencing to ensure that the library has retained gRNA representation...produce high titer lentivirus from the library, ensure gRNA representation in the viral prep, infect target... you're interested in studying. You need to make sure your assay is scalable and robust. What you don'...

  9. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...discuss those ideas and build from those.   Figure 1: Virtual lab meeting? No problem! You can still...final screen can also be saved and exported as a picture. I believe that showing something different from...document can be shared and serve as a springboard for future discussion. Collaboration and creativity are pivotal...

  10. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...got along well, suggest a coffee chat in the near future. Even just one more meeting (in person or virtually... but I’ll never forget the strawberries. Grand gestures are memorable! Attend events for one organization...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne...

  11. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...they also uniformly stepped back to give a big picture perspective: Dr. Yamanaka talked about his clinical...trial patients with advanced macular degeneration; Laurenz Studer discussed his work with Parkinson's Disease...presenters, it was gratifying to see that Addgene has ensured that plasmid acquisition is not a rate-limiting...

  12. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...predict protein structures, then you might know a thing or two about computer modeling. Make sure to focus on...? Or perhaps you have presented in some public lectures? These transferable skills could be meaningful...

  13. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...see the Introduction to Fluorescence Microscopy lecture at iBiology. Commonly used filter sets & relevant...this filter set and have reported brightness measurements. Here, EGFP outperforms the improved folding...variants, presumably due to the lower growth temperature. This also suggests, however, that there is no...

  14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...screening blog post for more information.   Figure 1: Result of a blue-white screen. Image from Stefan...more about colony PCR in this blog post.   Figure 2: The steps of colony PCR.   Sanger sequencing...in this case a plasmid. Sequencing is one of the surest ways to know that your insert is what you expect...

  15. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...and you think there are four important times to measure that growth rate, you report the results of all...between hypothesis testing and generation, can be sure that results will be reported regardless of outcome...the journal and reviewers are incentivized to make sure that any possible null is really a true null and...

  16. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...virulence. Scientists have studied how HGT occurs in nature and have learned how to introduce genetic materials...Transformation, transduction, and conjugation occur in nature as forms of HGT, but transfection is unique to ...material from the environment by bacterial cells. In nature, this genetic material often comes from adjacent...

  18. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...additional flexibility in pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.  ...nucleus through the nuclear pore complex. This feature may be useful for the transduction of terminally...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...

  19. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...results you've measured should change established theory. So, what can you do to ensure that your skills...our website and social channels to apply for our future events. In addition to this annual event, graduate...

  20. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...consolidate several steps into one. Soon you’ll figure out what prompts you need to consistently and accurately... accurately run a protocol.   Figure 1: Evolution of a Western blot protocol, from a full page ...experimental steps are worth doing, and doing well, so make sure you’re taking time to do them carefully and thoroughly...

  21. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...processes directly affected by light. Long-term light exposure can also lead to cellular phototoxicity, and experimental...substrate like coelenterazine is supplied in the culture medium to allow RLuc8 to produce photons. The original...wavelength to specify which FP emission you’ll capture. Since the Nano-lantern system does not use external...

  22. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...won’t work if the fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis...expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then...also well suited for insertions and deletions. The figure above shows guidelines for primer design; a desired...

  23. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...at all 1,500 or 1,600 transcription factors and figure out where they bind to the genome. We’ve been doing...other scientists quickly? Myers: We were hoping to figure out how to do this for many hundreds of transcription...as well. We would also like to put some social pressure on many other labs that may be doing something...

  24. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ... known as the resting potential (Vmem), can be measured as the imbalance of electrical charge between ...of the pHLuorin fluorescent protein. This GEVI features increased brightness and improved plasma membrane...Levin, Director of the Allen Discovery Center. The future of this field has a high potential for the advancement...

  26. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...These workshops illustrate the open and modular nature of the workshop content: different parts could ...for securing funding to support this effort. The future of reproducibility workshops and lessons learned.... Be on the lookout for upcoming workshops at future conferences this year. Workshops are confirmed ...

  27. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important...and rendered useless after less than a minute of exposure to the appropriate light. The photoactivatable...to engage a target sequence. Upon a brief light exposure (several seconds) the gRNA is released and the...

  28. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ..., with the expectation for major advances in agriculture, materials, chemicals, and medicine. As they’...and where all of it is likely to lead us in the future. Building circuits from CRISPRs Addgene: What...interactions. That’s relevant if you want to make sure you don’t target anywhere else, but for synthetic...

  30. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... have reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image... the spike protein was developed based on 100 structure-guided spike designs and has improved protein ...Maynard JA, Finkelstein IJ, McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-...

  32. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...Golden (Gate) pot (Pryor et al., 2022).     Figure 1: Workflow for high complexity Golden Gate Assemblies...cycling conditions (see Table 1).   Enzyme Temperature Incubation time/cycling Overhang length ...also optimized the thermocycler protocols and made sure that T4 ligase was the correct choice for the job...

  33. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...IgG2a antibody. When using a monoclonal antibody be sure to use a highly specific secondary antibody, rather... IgG2a>IgG2b>IgG1 (Manning 2012).      Figure 1: Broadly reactive versus subclass-specific antibodies...antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity when ...

  34. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... Level 1 TUs into multigene constructs. Figure 2: The three assembly levels of MoClo. Image courtesy...modification of these chemical products. The flexible nature of MoClo allows users to test a variety of different....pone.0016765.  Hahn, F., Korolev, A., Sanjurjo Loures, L. et al. A modular cloning toolkit for genome...

  35. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...including propensity for off-targets, secondary structures, etc., as well as the presence of a Protospacer...one of several Cas9s that recognize NG PAMs. The Nureki lab generated SpCas9-NG, a rationally engineered...lab sought to address the PAM problem through structure-guided engineering of the PAM-interacting domain...

  36. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...depending on the size of the conference). This made sure I didn’t zone out and kept me engaged throughout...If you’re presenting a poster at a conference, be sure to practice beforehand. Generally, two types of ...be on their website or the program booklet. Make sure you’re familiar with the policies before posting...

  37. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...available, you now face the challenge of trying to figure out what system might work best for your particular...vector reseals and other incorrect products. This feature biases the reaction in favor of the desired assembly...which one works best for you. Happy cloning (and be sure to deposit your finished constructs at Addgene!)...

  38. Of Myc and Men

    Type
    Blog Post
    ...of antibodies as tools allowed us to detect and capture proteins of interest to better study them in vivo...tagged protein rather than at the C-terminus. These features made this tag easier to use and more flexible ...will remain an important tool for the foreseeable future. These newer findings simply add to our understanding...

  39. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...heterozygote into a homozygote and ensuring that all future offspring inherit the endonuclease gene. As we ...address many pressing global problems in health, agriculture, and conservation. But please be aware that there...biosafety officer should be familiar with appropriate measures. Barriers should be a component of all gene drive...

  40. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...range of attention spans, skill levels, and STEM exposure. If you expect a lot of kids, you’re going to ... we can make this fun activity relate to STEM.” Figure 1 shows the activities we had for my school’s Math...Perhaps you have young children and you want to make sure their STEM education is supplemented. A 2009 study...

  41. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...vision in a canine model of childhood blindness. Nature Genetics 28, 92-95. PubMed PMID: 11326284. 4. Bennett...L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6. The Forever... Rev Virol. 2014 Nov;1(1):427-51. DOI: 10.1146/annurev-virology-031413-085355. PubMed PMID: 26958729.  ...

  42. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic diversity, to...Project: a global resource to map genomic diversity. Nature 604, 437–446 (2022). https://doi.org/10.1038/s41586...weaponization of genetics research by extremists. Nature 610, 444-447 (2022). doi: 10.1038/d41586-022-03252...

  43. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... mean a world of mental relaxation. And, I’m not sure I should put this in print, but I actually let my... my daughter skip school a couple of times. Be sure to add time with your family, for yourself and for...just “do science” – we are scientists. We have to figure out a way to keep being ourselves and to be happy...

  44. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...and S. cerevisiae (Tuller et. al., 2010). Figure 1: An overview of peptide synthesis. A ribosome...as Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that...PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and...

  45. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...The detectable signal from this reporter can be measured and quantified to determine the presence and quantity...lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK detection system...2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020...

  46. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...behavioral tasks was an elevated plus maze that measures risk aversion/anxiety. The mouse can move freely..., learning, and memory. We’ll cover these in a  future blog post, so stay tuned!  References Alpár, ...rapid and long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390...

  49. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ..., the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses three plasmids: (...in Medical Virology (Li et a., 2018).   Figure 2: To pseudotype with the SARS-CoV-2 spike protein..., Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti...

  50. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...Addgene. In vitro screening for the best gRNA Figure 1: Validation of double strand break (DSB) mediated...were fusing, you may have several mosaic mice. To figure out what alleles are present in these mice, you...favourite mammalian model organisms in the near future.  Many thanks to our guest blogger Samantha Young...
Showing: 751 - 800 of 1025 results