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We narrowed to 964 results for: app

Showing: 751 - 800 of 964 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... proteins.Therefore, GFP is not suitable for applications that require anaerobic conditions. In experiments...protein module that can report on location. This approach enables the study of proteins in cells, but not...

  2. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ... cell splitting issue at all if I’d planned appropriately. Back in my undergrad days, I had general goals... do I write things down? It’s more viscerally appealing for me to physically cross things out (it just...

  3. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...that expresses all desired variants of a protein. Applying a selective pressure winnows the pool down to ...technique described in Wrenbeck et al is a PCR-based approach for generating a customizable comprehensive mutagenesis...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...adjust this setting as needed. To figure out the appropriate voltages to observe your cells on a flow plot...voltage describes an electric potential that can be applied to the photomultiplier inside the cytometer to ...

  5. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help...budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz is a laboratory...

  6. Using a Light Microscope Protocol

    Type
    Protocol
    ...see your image through both sides. Once you are happy with the lighting, use the coarse focus knob to ...make minor adjustments to the focus. After you are happy with the positioning and focus of your image on ...microscope after use and store the microscope appropriately....

  7. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...something odd in our uncut DNA control: a band or two appear on an agarose gel at notably higher molecular weights... only in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for ...

  8. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...overnight culture of bacteria . Pro-Tip Refer to appropriate DNA prep protocol for volume of bacteria to grow...the plasmid DNA. Either spin to pellet the DNA or apply the solution to a column that will bind the now ...Aqueous DNA phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom...

  9. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https...

  10. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1 pathway...Dendreon Corporation is currently the only FDA-approved DC therapy for prostate cancer and is aimed at...

  11. Lentivirus ddPCR Titration

    Type
    Protocol
    ...complete: Prepare 7 microcentrifuge tubes with the appropriate amount of media volume listed in the chart below...tube. Add 4 µL of the 25 ng/µL samples to the appropriate PCR tubes. Pipette back and forth 10 times to...the average infectious titer obtained from the appropriate dilutions to calculate the final infectious titer...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...with the newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR...Laganière, J., Cheng, A. W., Hockemeyer, D., Gao, Q., Alagappan, R., Khurana, V., Golbe, L. I., Myers, R. H., ...

  13. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...within the entire construct. Is the primer an appropriate distance from the target? The primer should be... should I do? Check your trace file first; the apparent mismatch/mutation may be the result of a mis-called...

  14. Water Bath Protocol

    Type
    Protocol
    ...in place. Turn the water bath on and set the appropriate temperature as described in your protocol. How...in step 3. You will also need to maintain the appropriate water level in the water bath so before use, ...

  15. Ligation Independent Cloning

    Type
    Protocol
    ...traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide enough...with the start codon or tag sequences (where appropriate). The primer length is dependent on the T4 Pol...

  16. Gibson Assembly Protocol

    Type
    Protocol
    ...fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single isothermal...part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short to make its...

  17. Lentivirus Production

    Type
    Protocol
    ...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...mL of DMEM Complete to a 15 mL conical and an appropriate amount of 25 mM chloroquine to the conical so...

  18. Colony Formation Titering Assay

    Type
    Protocol
    .... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. Incubate the cells for ~2 weeks. ...replace it with fresh DMEM complete containing the appropriate antibiotic. When the untransduced (negative control...

  19. Pipetting Protocol

    Type
    Protocol
    ... P1000. The table shows the pipette with the appropriate dispense volume: This image shows the P2, P10...,000 µL Each pipette should be used with the appropriate tips, which are made in a number of sizes and...

  21. Western Blot

    Type
    Protocol
    ... can be adapted for wet and semi-dry transfer approaches. Last Update: January 24, 2022 Workflow Timeline...remove supernatant. Resuspend cell pellet in appropriate volume of cold lysis buffer. Pro-Tips The volume...

  22. AAV ddPCR Titration

    Type
    Protocol
    ...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... PCR tubes. Add 5 µL of dilutions 6–8 to the appropriate PCR tubes. Pipette back and forth 5 times. Lightly...

  23. Kit Free RNA Extraction

    Type
    Protocol
    ... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

  26. CRISPR Guide Update

    Type
    Blog Post
    ...introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources on the Addgene blog ...

  29. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

  30. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...questionable collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words ...

  31. We’re Going Viral This Week!

    Type
    Blog Post
    ... information on the basics of viral vectors, applications, and some new resources available from Addgene...

  40. Our New Antibody Mascot is....

    Type
    Blog Post
    ...rockin’ newly designed Addgene swag. Good luck and happy naming!   Fig. 2: The mascot trio!    ...
Showing: 751 - 800 of 964 results