Addgene

We narrowed to 394 results for: AGA

Showing: 301 - 320 of 394 results

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Simplify Cloning with in vivo Assembly

Type

Blog Post

...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Type

Blog Post

...with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant role in library...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry...
AAVs in Retinal Gene Therapy

Type

Blog Post

...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores...
A Guide to Starting Your Own Journal Club

Type

Blog Post

...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

Type

Blog Post

...plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration...
SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

...jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ...
Lentiviral Vector Uses and Overview

Type

Blog Post

...lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients had...
Plasmids 101: Colony PCR

Type

Blog Post

... complete, it’s time to run the products on an agarose gel to determine their size. Make sure to use an...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

...separate plasmids), there are a few reasons arguing against this approach. First, this would prevent the study...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

... possible to screen libraries of bait and prey against each other at the same time in order to try and...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...track your samples today and never lose a sample again! Amanda Hazen is the Laboratory Operations Manager...
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Type

Blog Post

...genome functions in the hope of developing drugs against intractable diseases. Currently, a number of labs...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Type

Blog Post

...molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial...
Negotiating Work and Life: How to Find the Joy

Type

Blog Post

...children, so my life will be under control.” Think again. Maybe you’ll need to care for an elderly family...
CRISPR 101: Any Base Transversion Editors

Type

Blog Post

...other transversion editors be close at hand? Once again, this post has a lot of acronyms, so we've included...
Tips for Writing a Good Cover Letter

Type

Blog Post

...manager may have. The closing paragraph should again show your enthusiasm for getting the position and...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

...RepB) necessary for replication in various hosts (Pagatto and Dillon 2001). The plasmid has its own replication...
Antibodies 101: Reading a Flow Plot

Type

Blog Post

...different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs are CD11c...
AAV Titers: Where Do They Come From And What Do They Mean?

Type

Blog Post

...reliable, or if the sample needs to be quantified again. Some of the issues with titrating by qPCR are ...

Showing: 301 - 320 of 394 results

Results per page:

Simplify Cloning with in vivo Assembly

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

AAVs in Retinal Gene Therapy

A Guide to Starting Your Own Journal Club

Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

SciComm: Taking Science to Elementary and Middle Schools

Lentiviral Vector Uses and Overview

Plasmids 101: Colony PCR

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Tips for Screening with Yeast Two Hybrid Systems

Tips from the Repository Trenches: Using Barcodes to Track Samples

Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Negotiating Work and Life: How to Find the Joy

CRISPR 101: Any Base Transversion Editors

Tips for Writing a Good Cover Letter

Plasmids 101: Broad Host Range Plasmids

Antibodies 101: Reading a Flow Plot

AAV Titers: Where Do They Come From And What Do They Mean?

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