We narrowed to 400 results for: AGA

1. Tips for Using BLAST to Verify Plasmids

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   ...result – blastx checks all six possible frames against the database. Another benefit is that a frameshift...

2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...other words, these parts can be used over and over again in combination with novel genes to get very precise...

3. Great Results Start with Great Standard Curves

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   ...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...

4. An Introduction to Adenovirus

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   ... Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930...

5. Viral Vectors 101: The Retroviral Lifecycle

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   ...emerges. Retrovirus life cycle Retroviruses go against the grain of the Central Dogma of Biology (gDNA...

6. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry...

7. A Guide to Starting Your Own Journal Club

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   ...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...

8. SciComm: Taking Science to Elementary and Middle Schools

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   ...jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ...

9. Simplify Cloning with in vivo Assembly

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   ...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...

10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant role in library...

11. AAVs in Retinal Gene Therapy

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    ...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores...

12. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration...

13. Negative Can Be Positive: Open AAV Data with Addgene

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    ...D-F)  High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...

14. Lentiviral Vector Uses and Overview

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    ...lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients had...

15. Plasmids 101: Colony PCR

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    ... complete, it’s time to run the products on an agarose gel to determine their size. Make sure to use an...

16. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...separate plasmids), there are a few reasons arguing against this approach. First, this would prevent the study...

17. Tips for Screening with Yeast Two Hybrid Systems

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    ... possible to screen libraries of bait and prey against each other at the same time in order to try and...

18. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ...track your samples today and never lose a sample again!   Amanda Hazen is the Laboratory Operations Manager...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...genome functions in the hope of developing drugs against intractable diseases. Currently, a number of labs...

20. Negotiating Work and Life: How to Find the Joy

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    ...children, so my life will be under control.” Think again. Maybe you’ll need to care for an elderly family...