We narrowed to 398 results for: AGA

1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   Blog Post

   ...with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant role in library...

2. A Guide to Starting Your Own Journal Club

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   Blog Post

   ...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...

3. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   ...plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration...

4. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry...

5. Negative Can Be Positive: Open AAV Data with Addgene

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   ...D-F)  High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...

6. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ...jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ...

7. An Introduction to Adenovirus

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   Blog Post

   ... Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930...

8. Viral Vectors 101: The Retroviral Lifecycle

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   Blog Post

   ...emerges. Retrovirus life cycle Retroviruses go against the grain of the Central Dogma of Biology (gDNA...

9. AAVs in Retinal Gene Therapy

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   Blog Post

   ...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores...

10. Great Results Start with Great Standard Curves

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    Blog Post

    ...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...

11. Lentiviral Vector Uses and Overview

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    ...lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients had...

12. Plasmids 101: Colony PCR

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    Blog Post

    ... complete, it’s time to run the products on an agarose gel to determine their size. Make sure to use an...

13. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    ...separate plasmids), there are a few reasons arguing against this approach. First, this would prevent the study...

14. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ... possible to screen libraries of bait and prey against each other at the same time in order to try and...

15. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...track your samples today and never lose a sample again!   Amanda Hazen is the Laboratory Operations Manager...

16. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...genome functions in the hope of developing drugs against intractable diseases. Currently, a number of labs...

17. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post

    ...molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial...

18. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ...children, so my life will be under control.” Think again. Maybe you’ll need to care for an elderly family...

19. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post

    ...squash" originated from the squash itself, but think again. Spaghetti squash is actually a component of the...

20. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ...favor a different model. Finding evidence for or against a model is a standard scientific strategy. However...