We narrowed to 382 results for: BRE

1. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...During DNA repair, there is a chance that the two breakpoints will repair without the section between the two...

2. Common Injection Routes in Mice

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   Blog Post

   ...patience, as you wait for the anesthetized mice to breathe in the liquid. Restraint: anesthesia Location: ...

3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ... Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S...

4. Transferable Skills Guide: Managing a Team

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   Blog Post

   ...) everything ourselves and it’s a hard habit to break. But as a manager you will be quickly overwhelmed...

5. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ...binding the protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can ...

6. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...sequence of events plays itself out: The researcher breaks eye-contact with any other human in the room. Their...

7. Plasmids 101: 5 factors to help you choose the right cloning method

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   Blog Post

   ...methods that make working with multiple inserts a breeze. Among them are some of the homology based methods...

8. Twenty Years of Addgene Sharing: CRISPR

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   Blog Post

   ...effectors. Nucleases generate a classic double-stranded break, while modulators regulate transcription and chimeric...

9. PhD Applications After COVID

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   Blog Post

   ...and video communication. But first, take a deep breath; at least you know you have an answer to that previous...

10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    .... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...

11. Experimenting with New Careers while in Grad School

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    Blog Post

    ...to get recharged. The fun change of pace helped break up the negativity and allowed me to focus on the...

12. Genome-wide Screening Using CRISPR

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    Blog Post

    ...-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through ...

13. Light Sheet Fluorescence Microscopy

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    Blog Post

    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...

14. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post

    ...small victory in a long road to your scientific breakthrough. More Reading: How to manage scientific data...

15. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations...

16. Neurodegeneration Plasmid Collection

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    Collection

    ...TEV-HsFbxo7-129-398/Skp1 FBXO8 GST, TEV tac Parkinson's Brenda Schulman 203365 Tau BA 12->7 K257T MAPT Flag CMV...Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's...

17. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    ...tools in molecular biology. “Retroviruses” is an umbrella term which encompasses 1.) the gamma-retroviruses...

18. Getting to Know Expansion Microscopy

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    Blog Post

    ...SRM), a family of imaging techniques developed to break through the resolution barrier while still utilizing...

19. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...of ~1.8 and an A260/230 of ~2.0. However, let’s break down how different contaminants impact these ratios...

20. Great Results Start with Great Standard Curves

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    Blog Post

    ...and the quality of a standard curve can make or break an experiment. Here, we will provide an overview...