We narrowed to 310 results for: CAD

1. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

   Type

   Blog Post

   ...DESKGEN (commercial, online), or ProtospacerWB (academic, offline). While it remains to be seen, I believe...

2. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

   Type

   Blog Post

   ...1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting module with the crRNA, (3) three...

3. Chemogenetics Guide

   Type

   Guide

   ...potently activated by an inert ligand. Proc Natl Acad Sci USA. 104:5163–68. PMID: 17360345 (Link opens...biological light sources for opsin activation. Proc Natl Acad Sci USA. 113:E358-67. PMID: 26733686 (Link opens...specifically designed Gi-coupled receptor. Proc Natl Acad Sci USA. 95:352–57. PMID: 9419379 (Link opens in...regulation of β cell function in vivo. Proc Natl Acad Sci USA. 106:19197–202. PMID: 19858481 (Link opens...

4. Adenovirus Guide

   Type

   Guide

   ... referred to as "gutless" or high capacity AdV (HCAdV) vectors. Here, all viral sequences are deleted,...accommodating ~36 kb of genetic cargo. Production of HCAdV vectors requires using an additional adenoviral ... adenoviral genes. The biggest challenge of the HCAdV vector production is to ensure no carryover from...recombinant adenoviruses . Proceedings of the National Academy of Sciences of the United States of America, 95...

5. 22 Hot Plasmid Technologies from 2014

   Type

   Blog Post

   ...they designed Opto-RTKs, which activate signaling cascades on exposure to low-intensity blue light. This ...

6. Gamma-Retroviral Vector Guide

   Type

   Guide

   ...been widely used in the research community for decades. Wildtype gamma-retroviruses have been engineered...-expressing cells. Proceedings of the National Academy of Sciences , 94 (16), 8640–8645. https://doi.org...

7. Viral Vectors 101: Systemic Capsids

   Type

   Blog Post

   ...Zheng, Q., Beddow, T., Zhao, B., Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg,...

8. Modular Cloning Guide

   Type

   Guide

   ...Plasmids for reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA...

9. Plan Your Experiment

   Type

   Guide

   ...manipulate the genome with relative ease. Over the past decade, researchers have expanded the CRISPR toolbox to...

10. Lentiviral Vector Guide

    Type

    Guide

    ...been widely used in the research community for decades. Wild-type lentiviruses have been engineered into...