We narrowed to 444 results for: ENA

1. Top Requested Lentivirus and AAV of 2016

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   ... various serotypes available (AAV2, AAV5, AAV8) enable different patterns of transduction and transgene...

2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   ...unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity and sgRNA...

3. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ...researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041...

4. AAV Q&A with Tim Miles

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   ...considering the need for some manner of polyA. In this scenario, you may be best served by a dual vector, split...

5. Antibodies 101: ChIP

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   ...native proteins (so antibodies validated only for denatured proteins may not be a good choice) and the amount...

6. Hot Plasmids - February 2022

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   ...enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by identifying...

7. Fluorescent Proteins 101: Luciferases

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   ...Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc® Luciferase Fluorescent Proteins 101...

8. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...variant, Phoenix-gp, contains only Gag-Pol and enables additional flexibility in pseudotyping.   ...

9. Antibodies 101: Flow Cytometry Controls

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   ...isotype control for that particular marker. This enables you to identify any non-specific binding to know...

10. How Addgene Creates Content

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    ...sharing speeds science, and our entire mission is to enable that process. Our repository of plasmids, AAVs,...

11. CRISPR 101: Non-Homologous End Joining

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    ...journal.pgen.1004086 Ghezraoui H, Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson...

12. Plasmids 101: Yeast Vectors

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    ... mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely...

13. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    ... that template for repair. By using these two scenarios, you can either knock out a gene or knock in a...

14. Visualizing Protein Turnover In Situ

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    ...segment is labeled with fluorescent probes that enable visualization of the location of the protein-protein...

15. Plasmid Tools for Microbiome Studies

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    ...process a large number of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome...

16. Lighting Up Cell Signaling with Photoswitchable Kinases

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    ...the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, suggesting PP1...

17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    ...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether...

18. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ... Broadening the spectral diversity of GEVIs can enable resolving single action potentials and further ...

19. Antibodies 101: Affinity Reagents

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    ...stable up to 80°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins...

20. Antibodies 101: Selecting the Right Antibody

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    ...antigen tend to work very well for applications that denature a protein to its primary (linear) structure using...