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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 372 results for: Fus

Showing: 301 - 320 of 372 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...collection page Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further... and of the fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein...MBP-TEV N-terminal fusions pDEST Gateway vectors - For the production of His6-MBP-tagged fusion proteins in ...vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more...insert a gene of interest. They may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible... by: Species-Specific Expression Epitope Tag or Fusion Protein Function: Viral Vector Delivery, Genome... vectors - Inducible expression, fusion proteins, and more pGBKT7-GW - Gateway...

  3. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene of interest fused to the...interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest fused to the...protein-protein interactions where each protein is separately fused to a donor or acceptor molecule (also referred to...a protein where the donor and acceptor are both fused to the same protein (also referred to as intramolecular...following plasmids can be used to create a fluorescent fusion protein with your gene of interest and the listed...mCerulean N1 Cyan Mammalian Express a gene of interest fused to the N-terminus of monomeric Cerulean mCerulean...mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus of monomeric Cerulean mTurquoise2...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...LumiScarlet : A fusion of LumiLuc (ATP-independent luciferase derived from teLuc) fused to mScarlet-I. ..., Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of a CMV ...ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2...ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale ...Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale...pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic...Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 106253 pLS-...

  6. Promega Plasmid Collection

    Type
    Collection
    ...vectors for sensitive gene expression analysis and fusion vectors containing tags such as NanoLuc, HaloTag... sensitive gene expression analysis and protein fusion vectors for detecting and tracking proteins using...high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link opens in a new window) is a 19.1 ...detection at endogenous expression levels. NanoLuc fusions are useful for both intracellular and extracellular...protein dynamics in highly scalable assays. HaloTag Fusions HaloTag (Link opens in a new window) technology...puts control of the function and properties of a fusion protein in your hands, thanks to interchangeable... mammalian cells, and cell-free systems. HiBiT Fusions HiBiT is a small, multifunctional epitope tag enabling...

  7. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers...Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine ...Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help visualize...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this fusion protein...examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy...Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various organelles...organelles or subcellular structures. You should fuse your protein of interest with a fluorescent protein that...Membrane CARMIL2 BH domain tdTomato John Cooper *Fusions to other FPs can be found by searching the complete...Microtubules ensconsin mCherry William Bement *Fusions to other FPs can be found by searching the complete...Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions to other FPs can be found by searching the complete...

  9. TALEN Plasmids and Kits

    Type
    Collection
    ...Kit Description Depositing PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate...for expression as TALE-LSD1 fusions. It was recently shown that TALE fusions with the histone demethylase...are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors...Takashi Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter... Gate TALEN and TAL Effector Kit. These modified pFUS vectors can reduce the number of module plasmids...Visualization [TGV]). pTALYM3 and pTALYM4 contain TALE fused with mClover and mRuby2, respectively, and can be...

  10. CRISPR Plasmids and Resources

    Type
    Collection
    ... dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information...peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure...across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a given...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...mStrawberry-N1 - Mammalian Expression FusionRed 580 608 18 Monomer FusionRed-pBAD - Bacterial Expression mRFP1...with different fluorescent tags for you to create fusion proteins with your gene of interest. Special... Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer not a Monomer...with different fluorescent tags for you to create fusion proteins with your gene of interest. These are ...Snapp . Empty Backbones for Fluorescent Protein Fusions (Organized by Color) For each table: Excitation...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...to Top Empty Backbones for Fluorescent Protein Fusions (Organized by Expression Species) Organism/Expression...

  13. Bacterial Expression Systems

    Type
    Collection
    ...tags so you can express your protein of interest fused to FPs and monitor their localization in live cells...Guide to Selecting Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable...test if two proteins bind or interact together by fusing each of them to two FPs whose emission and excitation...exciting the donor. In BiFC, proteins of interest are fused to each part of a split FP. When the candidate proteins...plasmids containing FPs and split FPs that can be fused to your proteins of interest in bacterial FRET and... Bacterial Two-Hybrid (B2H) System allows you to fuse proteins of interest to transcriptional regulatory...01 Promoter activity Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength...

  14. Plan Your Experiment

    Type
    Collection
    ...or Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their...copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair...editing guide RNA (pegRNA) Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a...permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB...permanently modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64...transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators that need to be within...

  15. Optogenetics AAV Preps

    Type
    Collection
    ...105669 pAAV-CKIIa-stGtACR2-FusionRed CaMKII GtACR2 (soma-targeted) FusionRed Constitutive 1, rg* Yizhar...105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed Syn GtACR2 (soma-targeted) FusionRed Cre dependent 1, 5, rg* Yizhar...105678 pAAV_hSyn1-SIO-stGtACR1-FusionRed Syn GtACR1 (soma-targeted) FusionRed Cre dependent 5 Yizhar 109048...

  16. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9...cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor...have been made by optimizing expression of the fusions, modifying the linker region between Cas variant...deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase...CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). ...

  17. CRISPR Plasmids - Plants

    Type
    Collection
    ... Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB.... pyogenes Yang 47024 pUC gRNA Shuttle Mt U6.6 inFusion none S. pyogenes Parrott 50591 pCBC-DT2T3 see ...

  18. Brain Initiative Collection

    Type
    Collection
    ...AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal...expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation Viviana Gradinaru...

  19. Fluorescent Protein Guide

    Type
    Collection
    ... Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When... and/or function of your protein of interest by fusing it to a fluorescent protein. FRET Find FRET pairs...resides by using a well-characterized fluorescent fusion protein. In Vivo Imaging Image with these powerful...FRET based biosensors - Pertz Fluorescent Protein Fusion Construct Set - Hamdoun Browse All Plasmid Kits...

  20. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Uncx Mouse Mouse IgG2a 114547 Anti-Mitofusin-2 [N171/17.4R] Mitofusin-2 Mouse Mouse IgG2a 114548 Anti-Mortalin...SNAPAP Human Mouse IgG2a 140072 Anti-Mitofusin-1 [N111/24R] Mitofusin-1 Mouse Mouse IgG2a 140073 Anti-Pannexin...Mouse IgG2a 225377 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a...IgG2a 225378 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a.../Tmem90b Rat Mouse 211603 Mitofusin-1 scFv [N111/24] N111/24 scFv Mitofusin-1 Rat Mouse 211604 Botch scFv...Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising a κ light chain...heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed by their insertion...
Showing: 301 - 320 of 372 results