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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 365 results for: Fus

Showing: 301 - 320 of 365 results

  3. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...mStrawberry-N1 - Mammalian Expression FusionRed 580 608 18 Monomer FusionRed-pBAD - Bacterial Expression mRFP1...with different fluorescent tags for you to create fusion proteins with your gene of interest. Special... Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer not a Monomer...with different fluorescent tags for you to create fusion proteins with your gene of interest. These are ...Snapp . Empty Backbones for Fluorescent Protein Fusions (Organized by Color) For each table: Excitation...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...to Top Empty Backbones for Fluorescent Protein Fusions (Organized by Expression Species) Organism/Expression...

  6. Optogenetics AAV Preps

    Type
    Collection
    ...105669 pAAV-CKIIa-stGtACR2-FusionRed CaMKII GtACR2 (soma-targeted) FusionRed Constitutive 1, rg* Yizhar...105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed Syn GtACR2 (soma-targeted) FusionRed Cre dependent 1, 5, rg* Yizhar...105678 pAAV_hSyn1-SIO-stGtACR1-FusionRed Syn GtACR1 (soma-targeted) FusionRed Cre dependent 5 Yizhar 109048...

  7. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9...cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor...have been made by optimizing expression of the fusions, modifying the linker region between Cas variant...deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase...CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). ...

  10. CRISPR Plasmids - Plants

    Type
    Collection
    ... Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB.... pyogenes Yang 47024 pUC gRNA Shuttle Mt U6.6 inFusion none S. pyogenes Parrott 50591 pCBC-DT2T3 see ...

  12. Brain Initiative Collection

    Type
    Collection
    ...AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal...expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation Viviana Gradinaru...

  13. Fluorescent Protein Guide

    Type
    Collection
    ... Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When... and/or function of your protein of interest by fusing it to a fluorescent protein. FRET Find FRET pairs...resides by using a well-characterized fluorescent fusion protein. In Vivo Imaging Image with these powerful...FRET based biosensors - Pertz Fluorescent Protein Fusion Construct Set - Hamdoun Browse All Plasmid Kits...

  14. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Uncx Mouse Mouse IgG2a 114547 Anti-Mitofusin-2 [N171/17.4R] Mitofusin-2 Mouse Mouse IgG2a 114548 Anti-Mortalin...SNAPAP Human Mouse IgG2a 140072 Anti-Mitofusin-1 [N111/24R] Mitofusin-1 Mouse Mouse IgG2a 140073 Anti-Pannexin...Mouse IgG2a 225377 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a...IgG2a 225378 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a.../Tmem90b Rat Mouse 211603 Mitofusin-1 scFv [N111/24] N111/24 scFv Mitofusin-1 Rat Mouse 211604 Botch scFv...Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising a κ light chain...heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed by their insertion...

  15. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...

  16. Optogenetics Guide

    Type
    Guide
    ...genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus upon light activiation... an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). ...point mutations, codon optimization, and chimeric fusion of domains from two different ChRs. These ChR variants... given protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1, light-stimulated...-part transcription factors - Cry2 and CIB1 are fused to a transcriptional activation domain and DNA binding..., both LOV and Dronpa can control activity of a fused protein through allosteric interactions. Researchers...cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated forms of cytosolic STIM1 to control...

  18. Tetracycline Inducible Expression

    Type
    Collection
    ...transactivator (tTA) was developed. tTA was created by fusing tetR with the C-terminal domain of VP16 (virion...etracycline-controlled t rans a ctivator) was created by fusing rTetR with VP16. The tetracycline on system is ...CMV-driven; linked via IRES to EGFP-Blasticidin fusion; pMA2641 has rtTA driven by retroviral LTR promoters...than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression...

  19. Cloning

    Type
    Guide
    ...overhangs, or which are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ...different molecular biology techniques (such as fusing it with different tags, putting it under a variety...ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ end. The topoisomerase acts as a ligase...molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments.... Similar to Gibson, this method can efficiently fuse two (or more) fragments of dsDNA that have 30 or... of DNA that share no end homology can still be fused together in a seamless manner. To accomplish this... (or more) fragments of DNA that you would like fused along with custom ordered DNA oligos of 60-80 base...

  20. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation...mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes...
Showing: 301 - 320 of 365 results