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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 627 results for: PROC

Showing: 301 - 320 of 627 results

  1. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...Protein expression refers to the second step of this process: the synthesis of proteins from mRNA and the addition...Plant systems share many of the same features and processing requirements as mammalian cell expression systems...

  2. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than... carbohydrates that can inhibit the cell lysis process. To ensure that lysis is complete, use a reagent...

  3. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...genes or entire pathways in a particular disease process. While CRISPR is certainly not the first means ... screen using CRISPR libraries is a multi-step process (see Fig. 2). In most cases, CRISPR libraries are...

  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Therefore, these can be used as “timers” for cellular processes following their activation. The four main FTs ...Throwing color lights on molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov ...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    .... During the propagation and/or transformation process, some bacteria will either never receive a copy...antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp only affects...

  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556...

  7. DNA Purification Without a Kit

    Type
    Blog Post
    ...and without degradation during the extraction procedure. Additionally, purified DNA was free of agarose...analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences of the United...

  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...one is needed. For these reasons, we are in the process of transitioning our titering method from qPCR ...information needed for your PCR experiment. The process to titrate AAV by ddPCR begins with diluting the...

  9. MXS Chaining

    Type
    Blog Post
    ...various mammalian promoters), the same cloning procedure can be used to incorporate each element into the...and protein localization, as well as cellular processes like gene expression and cell migration (Sladitschek...

  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...import and export regulations, differing customs processes, and the distances the packages needed to travel...addgene.org. Currently, we’re evaluating our depositor process to identify and reduce barriers for scientists ...

  11. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...transcript by endogenous processing enzymes (Figure 1). To ensure this tRNA processing only occurs on the RNA...

  12. How to Be an Excellent Trainee

    Type
    Blog Post
    ... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...training, while others prefer to review their notes, process what they’ve just learned, and ask questions later...

  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...to immunoprecipitation and simply refers to the process of anchoring an antibody, often called the capture...protein interactions, and limit high throughput procedures. However, agarose beads are often a more cost-effective...

  15. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...sample name. If there was a minor error during processing, the window shows a yellow checked circle. Typically...results. If there are no results or there was a processing error, you will see a red exclamation point in...

  16. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...using Gateway technology To better understand the process, we’ll walk through an example experiment where...directly into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...

  17. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...others for failing to find our work?  I study processes for diagnosing and resolving organizational performance...impact of their contribution. It offers a 5-step process and several examples for creating effective research...

  18. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...You can see some of Joanne’s comments on the AMA process below, but we also wanted to share some thoughts...submission to avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out...

  19. Lab to Office Culture Shock

    Type
    Blog Post
    ...the following email from a friend: “I'm in the process of onboarding a new employee who's coming directly...is good. You can experience a real onboarding process. Unless you were really lucky with your academic...
Showing: 301 - 320 of 627 results