Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 556 results for: SPR

Showing: 301 - 320 of 556 results

  1. Bacterial Expression Systems

    Type
    Collection
    ...list of our bacterial CRISPR plasmids, see here . pwtCas9-bacteria 44250 CRISPR Stanley Qi Anhydrotetracycline... list of our bacterial CRISPR plasmids, see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of...alter bacterial genomes. From recombineering to CRISPR , this collection contains a variety of tools to...research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini Bacterial expression of Cas9... For a more comprehensive list of our bacterial CRISPR plasmids, see here . pGRG Series Various Plasmids...

  2. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...among those mentioned above. The contaminants can spread through dirty clothing, lab wears, human speech...increase the risk of contamination. Mycoplasmas can spread from these sources through cross-contamination ...handling or poor lab techniques, mycoplasma can spread through this form of powdered media. Filtration...working and discard them after use. Human contact can spread contamination when you are working without gloves...

  3. Important Considerations When Using AAVs

    Type
    Blog Post
    ...system. Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...neuroanatomical tracing techniques: exploiting the offspring of genetic engineering." Journal of neuroscience...Gene Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional ...

  4. Neurodegeneration Research Collection

    Type
    Collection
    ...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors...about the blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons...plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein. Sastre et al..... Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent...

  5. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    ...breakdown Intro Chapter 1 - Introduction to Mark Springel, Olivia Ho-Shing, and the Journal of Emerging ...Many thanks to our guests,Olivia Ho-Shing, Mark Springel, Arooba Ahmed, Jiachen Lee, and Jillian Parker...teach students about basic plasmid biology Use or CRISPR 101 eBook to introduce students to this amazing...

  6. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids...ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more (find the full, searchable list...

  7. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...choice. For more help with CRISPR, read our recent blog post explaining how CRISPR is being used for bacterial...Check out our Genome Engineering Overview and our CRISPR guide to find out how to use genome editing techniques...

  8. Call for Guest Bloggers

    Type
    Blog Post
    ... in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L...Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists...

  9. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...1 to 10 Fill in experimental details on the spreadsheet: Name your viral prep Enter the specific names... plasmids Enter plasmid concentration The spreadsheet will calculate the following μg of each plasmid...with its corresponding column in the calculator spreadsheet: We hope that this calculator will make it easier...

  10. Resolute Plasmid Collection

    Type
    Collection
    ...Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled libraries are useful ...Human SLC Activation Library 132561 Superti-Furga CRISPR activation library targeting human SLC proteins...Human SLC Knockout Library 132552 Superti-Furga CRISPR knockout library targeting human SLC proteins. ...Mouse SLC Knockout Library 141436 Superti-Furga CRISPR knockout library targeting mouse SLC proteins. ...Knockout Libraries 213695 221409 221410 Superti-Furga CRISPR knockout library targeting human transmembrane ...

  11. Editor's Choice, October 2016

    Type
    Blog Post
    ...Click on the links below to catch up on ways to use CRISPR in plants, to learn techniques for detecting genome...Posts from October 2016: Sequencing Options for CRISPR Genotyping Grad School Advice Part 2: Building ...Building Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business...

  12. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... infiltration of CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited...postdocs, which accounts for many, many lattes and espressos. The energy boost is well needed to keep up with...

  13. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... Based on CRISPR/Cas by Shravanti Suresh Guest blogger Shravanti Suresh who studies CRISPR at Iowa State...State University detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids...

  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...mitosis. Nat Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human CRISPR activator system for reprogramming human...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ...enogenous genes Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski... Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-Mediated Differentiation...

  16. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...induced pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations...looking at a bunch of different strategies with CRISPR-Cas9. There are so many different variants and ...functional role of those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse...

  17. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...vector for seamless joining of multiple mutations spread over thousands of base pairs. Cloning sgRNAs Generating...Generating constructs to produce sgRNA for CRISPR experiments can be laborious, requiring multiple cloning... Addtional Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400 citations in its first...much, you can see the 2013 ‘bump’ related to the CRISPR/Cas papers and Addgene deposits, but papers published... Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (2013): 819-823....

  19. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering...contrast to using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering... et al. "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643...

  20. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...rough waters of mutation validation, such as this "CRISPR 101" post. Next-generation sequencing technologies...High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology... crispr...
Showing: 301 - 320 of 556 results