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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 310 results for: bon

Showing: 301 - 310 of 310 results

  1. Adenovirus Guide

    Type
    Guide
    ...pAdEasy-1 Adenoviral backbone plasmid that lacks E1 and E3. pAdEasy-2 Adenoviral backbone plasmid that lacks...transgene of interest and both ITRs. Adenoviral backbone plasmid (e.g. pAdEasy) — containing the adenoviral...containing pAdEasy. pAdEasy is a ~33 kb adenoviral backbone plasmid containing the adenoviral genes necessary...production. The shuttle plasmid and the adenoviral backbone plasmid have matching left and right homology ...recombination of the transgene into the adenoviral backbone plasmid. One can also co-transform standard BJ5183...transgene has been inserted into the adenoviral backbone plasmid, and that other patterns of recombination...equivalent across multiple viruses. The adenoviral backbone plasmid pAdEasy-1 (Addgene #16400) is suitable...

  2. CRISPR Guide

    Type
    Guide
    ...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells... gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions between the gRNA...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading and discrimination...endonuclease. Nature , 513 (7519), 569–573. PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson...system. Nature , 493 (7432), 429–432. PMID: 23242138 Bondy-Denomy, J., Garcia, B., Strum, S., Du, M., Rollins... Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa...Marino, N. D., Pinilla-Redondo, R., Csörgő, B., & Bondy-Denomy, J. (2020). Anti-CRISPR protein applications...

  3. Cloning

    Type
    Guide
    ...Techniques You may also like... Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme...compatible restriction enzymes, that cut your insert or backbone at only the desired location for your cloning ...putting it under a variety of promoters and into backbones with different selection cassettes). Although ...disadvantage of TOPO® cloning is that very few plasmid backbones are available TOPO® ready, and it is not feasible...overhangs can be efficiently ligated into any plasmid backbone independent of how it was originally designed ...

  4. Molecular Biology Reference

    Type
    Guide
    ... for an empty plasmid backbone for your experiment, see Addgene's empty backbone page for more information...of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can...unique and not located elsewhere in the plasmid backbone, which is why they can be used for cloning by ...Antibiotic Resistance Choosing Your Perfect Plasmid Backbone 6 tips for analyzing and troubleshooting sanger...

  5. Chemogenetics Guide

    Type
    Guide
    ...23303063 (Link opens in a new window) Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez...RJ, Richie CT, Harvey BK, Dannals RF, Pomper MG, Bonci A, Michaelides M (2017). Chemogenetics revealed:...Link opens in a new window) Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez MH, Hu X, Galvan...

  6. Plan Your Experiment

    Type
    Guide
    ...off-target editing is a major concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the... to generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and ...

  7. Science Guides

    Type
    Guide
    ... Plasmids within a given library have the same backbone, but they express or target different genes. Some...

  8. Guide to Using Pooled Libraries

    Type
    Guide
    ... Plasmids within a given library have the same backbone, but they express or target different genes. Some...

  9. Sequencing Primers

    Type
    Guide
    ...repository, we've chosen primers based on the plasmid backbone and insert. To identify primers that may be useful...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...there are other envelope gene options, including: Gibbon Ape Leukemia Virus (GALV) — T and B cell transduction...
Showing: 301 - 310 of 310 results