We narrowed to 401 results for: caps
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TypeBlog Post...but this also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation...
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CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...synthetic polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021. Alternative... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...Sequencing (CPS) workflow and yield sufficient data to capture the entire viral genome. The full details of that... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...focused on modifying the outside protein shell, or capsid, of the virus so that it only enters and delivers... -
Tips for Technical Support Calls
TypeBlog Post... science, but we don’t have the resources to recapitulate the experiments performed by our thousands of... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...technologies that has transformed the assay’s capabilities. Specifically this includes primer directed ... -
Plasmids 101: Protein Expression
TypeBlog Post... work with than mammalian cells, and are often capable of modifying complex proteins unlike bacterial ... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...residues D10 and H840. To ensure that the clones were capable of cleavage, they tested the clones against an ... -
Transferable Skills Guide: Managing a Team
TypeBlog Post..., find out what their learning styles are and capitalize on them for the benefit of the team. By properly... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the biotinylated target... -
Of Myc and Men
TypeBlog Post...of antibodies as tools allowed us to detect and capture proteins of interest to better study them in vivo... -
Hot Plasmids: Winter 2025
TypeBlog Post...PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...through DNA degradation, thanks to the unique capabilities of Cas3 and its partner in crime, Cascade. Find... -
3D Printing Meets CRISPR Cas9
TypeBlog Post... of Cas9 – primed for cleavage. This structure captures the displaced non-target DNA strand and shows ... -
Academic vs. Industry Postdocs
TypeBlog Post...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...undifferentiated “stem” state and, Their potency or capability to differentiate into specialized cell types ... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...institutions recently reported their inability to recapitulate the results of Gao et al 2016 in a letter to... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post... YFP, cyan and yellow variants of EGFP equally capable of dimerizing as EGFP. As a result, CFP and YFP... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...other people to do that too. We don’t have the capacity to send tools out to people ourselves, but we’... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...wavelength. Thus, FPs are proteins with this unique capacity. Many of these FPs are fluorescent when ectopically...