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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 328 results for: hal.1

Showing: 301 - 320 of 328 results

  2. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...for RaPID is an RNA motif of interest. See figure 1 below for details on the key components of RaPID. ...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ... an entirely new affinity reagent.    Fig. 1: A comparison of an antibody (left) to several alternative...reduced-in-size options often have reduced binding strength, half-lives, and/or specificity as well. They’re more ...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... Science Find the SEND plasmids    Figure 1: SEND combines an endogenous Gag homolog, cargo RNA...cavity of Cas12f. The CasMINI system is less than half the size of the Cas9 or Cas12a based systems, making...

  5. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...many of which are available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity...strand synthesis. In contrast, a scAAV vector with half the size of the ssAAV genome has a mutation in the...

  6. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ... yellow light activates halorhodopsins to silence neuronal activity (Figure 1) (Wiegert et al., 2017)....bacteriorhodopsin; a comparative study of strains of Halobacterium halobium differing in pigmentation." Biochemical...Rhodopsin-like protein from the purple membrane of Halobacterium halobium." Nature new biology 233.39 (1971): 149...opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins, the first two found...of the DA neurons produce behavioral and electroencephalography evidences of arousal in mice previously...release for more information. References Abdallah, Khaled, et al. "Adeno-associated virus 2/9 delivery of...

  7. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...collaborations, keep these jobs in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...research-intensive careers. In addition, this skill is a hallmark for jobs focusing on: Science Policy, Business...

  8. dTAG - You're it!

    Type
    Blog Post
    ...cereblon, leading to rapid target degradation (Figure 1). Plasmids: We generated a series of plasmids that...molecules by conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867...

  11. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ-3. This system is notable for several... is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact...

  13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...off-target effects (Zibitt et al. 2021).    Figure 1: A schematic illustration of CRISPR/Cas9-based approaches...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ...

  15. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...target DNA quickly and accurately.  Figure 1: Steps in PCR reaction   This process of denaturation...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

  16. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...for your first journal club to begin! Figure 1: Discussing next-generation sequencing at the Addgene...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...

  17. Finding Your Perfect Job After University

    Type
    Blog Post
    ...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in the...become bored because I was not being mentally challenged. For these and other reasons, I started  looking...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like...

  18. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...can make this fun activity relate to STEM.” Figure 1 shows the activities we had for my school’s Math &...much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad...

  19. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...successful and meaningful career in the grants profession: 1. Build on your existing skills & passion  Skills such...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

  20. How to Write a Scientific Review Article

    Type
    Blog Post
    ...done is better than perfect. Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...
Showing: 301 - 320 of 328 results