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Showing: 301 - 320 of 954 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific... well. Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus...

  2. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    Blog Post
    ...precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination...system that can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing...labeling Isaacman-Beck et al., bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for... Diessl et al., Microbial Cell, 2020. https://dx.doi.org/10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent... tTALE-FP Shin et al., Sci. Rep. 2019. https://doi.org/10.1038/s41598-019-53722-0 ...

  3. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post
    ...and impact of your work. Please email deposit@addgene.org if you would like to contribute to the catalog...share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If you have suggestions...immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone, ME...Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030    Bradbury A, Plückthun...antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a  Resources We would also like to...

  4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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    ...Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito...reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585   Ding T, Huang C, Liang Z, Ma...Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321-4   Kolodner R (1980) ...National Academy of Sciences 77:4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank...efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118 Additional resources on ...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post
    ...are better for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred...involve enhancing optical live imaging of proteins, organelles, and cells. Similar to FPs, PA-FPs allow for ...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion, etc...extraordinary molecular detail.   References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable...mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during...Catch Up on Your GFP Background Resources at Addgene.org Find Photoactivatable FPs in Empty Backbones...

  6. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in...visualization of FP attributes (which turned into www.fpvis.org).  That page has been very useful over the years... and dissemination of information about FPs. FPbase.org FPbase is a project written in Python & Django...(so feel free to moderate your own version of fpvis.org with the proteins of your choosing!), and they...Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent protein plasmids Find...

  7. Cultivating Community Science at BosLab

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    Blog Post
    ...official by registering BosLab inc. as a nonprofit organization in the state of Massachusetts. Because we’re...local companies and BosLab has grown into an organization consisting of over twenty members and volunteers... involved, check out the local groups page on DIYBio.org to find a community lab in a city near you. To...Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville...Plasmid Sharing Speeds Science Resources at Addgene.org Order Plasmids from Addgene! Find Free and Open...

  8. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...support. “It is an exciting and emotional time!” George Church tries to recruit people who are nice as ...“Treating people fairly does not mean equally.” George Church was surprised by “how much better it is ... to lead his team as a scientists and a friend. George Church runs his lab fairly similarly to his advisor... Or share your success stories below. And don't forget to stay tuned for the last post in this series ...Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking the ...

  9. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ... your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other...in Cell and Developmental Biology 6: . https://doi.org/10.3389/fcell.2018.00054 Sander JD, Joung JK ...genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard T, ...of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722 Additional resources ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

  10. Antibodies 101: Monoclonal Antibodies

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    Blog Post
    ...functional variable regions. mAbs 10:539–546. https://doi.org/10.1080/19420862.2018.1445456  Holzlöhner P, Hanack...Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832  Lipman NS, Jackson LR, Trudel LJ...Information Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron... Biological Chemistry 291:20993–21007. https://doi.org/10.1074/jbc.m116.736942  National Research Council...monoclonal antibodies for immunotherapy Resource on Addgene.org Check out Addgene’s antibody plasmid collection...

  11. OCaMP: A New Calcium Indicator for Neural Imaging

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    Blog Post
    ...https://www.addgene.org/browse/article/28248224/","href_with_scheme":"https://www.addgene.org/browse/article...scaffold of OCaMP) are shaded in orange. Data from FPbase.org. Scientists have developed calcium indicators...activity. bioRxiv 2025.07.28.667269; doi: https://doi.org/10.1101/2025.07.28.667269. Additional Resources...Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids In-...

  12. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ...GPCRome. Nat Struct Mol Biol 22:362–369 . https://doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP,...Proximity Labeling. Cell 169:338-349.e11 . https://doi.org/10.1016/j.cell.2017.03.028 Roth BL (2016) DREADDs...for Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Takeda S, Kadowaki...genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional resources...our quick guide to chemogenetics Resources on Addgene.org Check out Addgene's chemogenetics guide Find...

  13. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...2020). https://pubs.acs.org/doi/10.1021/acscentsci.9b01093 More resources on addgene.org  View Addgene's...cells. Nat Biotechnol 33, 985–989 (2015). https://doi.org/10.1038/nbt.3290 Rozners, E. Chemical Modifications...Frontiers in Genome Editing, 28 (2021). https://doi.org/10.3389/fgeed.2020.617910 Zou, R., Liu, Y., Wu,...Molecular Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori, E., Satyapertiwi...

  14. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post
    ...National Academy of Sciences 104:5163–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa J, Broek...addition method. Mol Cell Biol 8:2159–2165 . https://doi.org/10.1128/mcb.8.5.2159 Galvan A, Raper J, Hu X, Paré...monkeys. Eur J Neurosci 50:2801–2813 . https://doi.org/10.1111/ejn.14429 Kimple ME, Brill AL, Pasker RL...Current Protocols in Protein Science 73: . https://doi.org/10.1002/0471140864.ps0909s73 Additional references...the many types of protein tags References on Addgene.org Check out Addgene's Chemogenetics Guide Find...

  15. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post
    ...monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating...by a lack of nutrients and targets cytosol and organelles to the vacuole/lysosome for degradation and recycling...more fine-grained detail about what proteins or organelles are being degraded) and it is slow. Alkaline...for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy,4(2), 205-213. doi:10.4161...Fluorescence Microscopy Additional Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent...

  16. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ... acid bases already present throughout living organisms, Click Biology reactions can add new functionality...Addgene in multiple formats: for use in different organisms, targeted to particular compartments, or fused... Chemical Reviews, 121(12), 6697–6698. https://doi.org/10.1021/acs.chemrev.1c00469  Howarth, M. R. (2025...biological reactions. Nature Chemical Biology. https://doi.org/10.1038/s41589-025-01944-x   Additional Resources...Plasmids 101: Degron Tags Additional Resources on addgene.org New England Biolabs Cell-Imaging Plasmid Collection...

  17. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...Students Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check Out Our ...

  18. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post
    ...tech transfer team will communicate with your organization’s technology transfer office or other authorized...than 75 plasmids, please contact us at deposit@addgene.org. These deposits are considered large deposits... your PI and send the spreadsheet to deposit@addgene.org with the following information: Addgene account.... The name of the Principal Investigator and Organization where your plasmids were first created. From... You'd Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which Plasmids...

  19. Plasmids 101: Degron Tags

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    Blog Post
    ...E3350-7. doi: https://doi.org/10.1073/pnas.1216880109  Resources on Addgene.org CRISPR Plasmids – Protein...studies. Nat Commun. 13, 5495 (2022). doi: https://doi.org/10.1038/s41467-022-33246-4  Chung HK, Jacobs CL...Chem Biol. 2015 Sep;11(9):713-20. doi: https://doi.org/10.1038/nchembio.1869  Nabet B, Roberts JM, Buckley...Chem Biol. 2018 May;14(5):431-441. doi: https://doi.org/10.1038/s41589-018-0021-8  Holland AJ, Fachinetti...

  20. 10 Basic tips for mammalian cell culture

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    ...culture that will hopefully help you keep a well organized lab and contamination free environment for successful...should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure...consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin)! It’s ..."Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis, and gene expression disregulation...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...
Showing: 301 - 320 of 954 results