We narrowed to 883 results for: prot

...antibodies against popular fluorescent proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also.... Antibodies are the most widely used class of protein-binding reagents. In the lab, their binding specificity...specificity allows scientists to target proteins of interest for labeling, quantification, purification...distribution funds our operation. Antibodies are glycoproteins that are part of the humoral immune system. ...complex antibody chips that allow assessment of many protein targets in a single sample; 3) producing recombinant...

...the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test...test varies in the sequence of steps and the Cas protein used. Here, all the components needed for the reaction...40 min incubation. A scalable, easy-to-deploy, protocol for Cas13-based detection of SARS-CoV-2 genetic...Assay (FELUDA) is a technique that uses FnCas9, a protein that is highly sensitive to the presence of mismatches... acid detection with CRISPR nucleases. Nature Protocols 14:2986–3012 . https://doi.org/10.1038/s41596-...Wilson, M. Z. (2020). A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic...Zhang, F., Abudayyeh, O. O. & Gootenberg, J. S. A protocol for detection of COVID-19 using CRISPR diagnostics...

...primarily characterized by protein misfolding that leads to a build-up of protein aggregates termed “lewy...those that overexpress the toxic protein α-synuclein (the most common protein found in lewy bodies), have ...scientists to easily observe fluorescently labelled proteins and tissues to better assess developmental processes...modification, such as expression of fluorescent protein or disruption of a gene, can be easily visualized...quick guide to Drosophila Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources...

...companion post describing protein expression strains, where we go over the basics of protein expression in E. ...your gene, and may have even put a fluorescent protein downstream, separated by an IRES element. You did...general lab strains designed for subcloning or protein expression. If you were to include customized strains...gene Increases the stability of certain expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage...fertility plasmid. A low copy-number plasmid, encodes proteins needed for bacterial conjugation. Genes listed...stringent factor Allows RNA synthesis in absence of protein synthesis Ptrc-ccdA   Propagation of ccdB-containing...

...vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional regulators. In this...and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore, targeting...Find the GeneWeld vector kit CasX, a small Cas protein, is active in E. coli and human cells Two years...ago, Jennifer Doudna’s lab discovered a new Cas protein, CasX, from metagenomic analysis of microbial DNA...Cas9 to a mutated human estrogen receptor. This protein fusion stays in the cytoplasm, but with the addition...

...covalent modifications to DNA or histones, the proteins that help package DNA, were associated with gene...With the help of guide RNAs, fusions between Cas proteins and the epigenetic modifier could be targeted ...cytosine demethylation of DNA. However, several proteins in the DNA oxidation and repair pathways work ...NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging into viral...exhibits increased PAM flexibility, and this fusion protein allows for LSD1 targeting to a wider set of genomic...methylation in vivo using an engineered dCas9-MQ1 fusion protein. Nature Communications 8: . https://doi.org/10.1038...endogenous genes using programmable TALE-TET1 fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org...

... purification or if you use a DIY purification protocol, you might have noticed that there are many options...options to elute your DNA prep. You might see protocols that recommend eluting in water, Tris-EDTA (TE...purification from E. coli is the presence of the protein endonuclease I encoded by the gene endA. Nowadays...are usually extra steps like heat inactivation, protein degradation, and/or the use of chelating agents...agents that can be introduced in the purification protocol to remove endonucleases activity. For these reasons...Check out the Molecular Biology Reference Find protocols on plasmid cloning Find plasmids for your research...

...? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead...Research Campus reminds us that, although fluorescent proteins are incredibly useful, chemical fluorophores can...Zika virus and, upon binding, produce reporter proteins. These toehold switches can be used to quickly...University, describes R-bodies, “force generating protein machines that can extend and retract over many ...different pH’s and some that are fused to fluorescent proteins. She also showed that R-bodies can rupture E. ...Casilio, a system that uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety...

...BBB via interactions with a similar protein called LY6C1. This protein is expressed in both BALB/cJ mice...exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While...as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... ... it, cross the BBB through an endothelial cell protein called LY6A (Hordeaux et al., 2019; Huang et al...capsids that rely on interactions with those two proteins to cross the BBB are often not effective in NHPs...in rats. For more information on reagents and protocols, visit https://clover.caltech.edu. Figure adapted... selected for its ability to bind to the LY6C1 protein in order to reduce strain-specific tropism in mice...

...right)est Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids...tool in neuroscience research. These fluorescent proteins light up in response to calcium, which is more...Aggarwal et al., 2025), based on the fluorescent protein mOrange2 (Figure 2). Figure 2: OCaMP structure...various red and yellow GECIs measured with soluble protein. Reproduced from Aggarwal et al. 2025 under a CC-BY-NC-ND... OCaMP, including detailed characterization as protein or in neuronal cultures, zebrafish, and mouse brain...

...ability Experimental guidelines and applications Protein structure and properties Links to relevant resources...a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...performing a viral delivery of Cas, the size of the protein would be helpful to know for viral packaging limitations..., RefSeq ID, etc.) and you will see a table of protein entry results. For the advanced search options,...from Adler et al.   You can also search by protein sequence with BLAST, using the homepage search ... them by other parameters, such as E-value for protein sequence searches. Additionally, the Phylogeny ...

...experiment check out Addgene’s protocol page, which has both protocols and videos for techniques in basic...about making your own competent cells and our protocols page to learn about bacterial transformation in...example, this plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green....plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find our molecular biology...biology reference here Find molecular biology protocols here ...

... tumor microenvironment produce CTL associated protein 4 (CTLA-4), a receptor that turns off T cell function...Furthermore, tumor cells alter their cell-surface proteome to inhibit expression of co-stimulatory molecules...major histocompatibility complex (MHC) class I proteins on their surfaces to avoid recognition by CD8+...formation and metastasis, block the cell-surface proteins that help escape immuno-surveillance, and curb...Antibodies that block cell-surface tolerance-producing proteins are one of the most commonly used immunotherapeutic...-L1 pathway, VEGF production, and CTLA4 and CD protein blocking. Other molecular targets under research...altering tumor cells and immune cells to knockout proteins involved in immune tolerance. Recently, China ...

...tracing with fluorescent proteins in nontransgenic and cre driver mice. Current protocols in neuroscience / ...longer available through Kopf). Additional general protocols on stereotaxic surgeries for viral injections ... Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification of opsin ...specificity. Viruses that only express the fluorescent proteins are used as a critical control. Depending on ...using combinatorial viral approaches. Current protocols in neuroscience / editorial board, Jacqueline ...plasmids! Find AAV resources! Find fluorescent protein resources!    ...

...Addgene to set up assays to see what molecules or proteins are involved in IL-36 release in keratinocytes...How GNB1 mutations affect signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside...responses. For example, GPCRs and their downstream G protein partners can regulate ion channels in the brain...standing interest in the biology of GPCRs and G proteins, with an emphasis on oligomerization, signalling...science education at McGill University.  These protein-receptor complexes consist of many parts that work... GNB1 gene, which codes for G𝛃1 subunits of G protein complexes, lead to a rare type of developmental...

... Examples of some genes encoding gap junction proteins cx43 GJB1 GJB2 Bioelectric states can alter ... even between many cells (through gap junction proteins which connect the cytoplasm of two cells) can ...controlled by the opening and closing of ion channel proteins, they are not always easy to track at the transcriptional...University has used Arch (green-light activated proton pump) and ChR2 (blue-light activated cation channel... a mutated version of the pHLuorin fluorescent protein. This GEVI features increased brightness and improved...J., et al. "Long-range neural and gap junction protein-mediated cues control polarity during planarian...

... RNA protectors epegRNAs: epegRNAs include structured motifs at their 3′ end to protect them from...This editor includes the fused RNA-binding protein La to protect the end of the pegRNA. Adding 3′ polyU tracts...PE7 (right) offer two different strategies for protecting pegRNAs from degradation. Created with BioRender.com...prime editing with an endogenous small RNA-binding protein. Nature, 628(8008), 639–647. https://doi.org/10.1038...

...stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI:10.1038/nprot.2016.171 Zhao, L., Koseki, S.R.T...or non-existent PAM requirement, and how these proteins are advantageous in today’s genome editing landscape...structures, etc., as well as the presence of a Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence...requirements. Luckily, there are some available.   Cas proteins that don’t utilize NGG   There are plenty of Cas9...developments below!) (Walton et. al, 2020). These Cas proteins have everything we know and love about Cas9, but...

...Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted Genome Editing (...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...identified a highly bright and stable fluorescent protein, StayGold. Although it quickly saw wide interest...et al. (2023). A monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org/10.1038...2023). Bright and stable monomeric fluorescent proteins derived from StayGold. ResearchSquare (Preprint...Whether you plan to purify or image a recombinant protein, the FLAG tag has proven itself a good option for...

...application, like protein production. Titration of baculovirus can vary depending on the protocol used. Plaque... viruses, though they are a popular choice for protein production. The most commonly used cells in insect...is what makes insect cells a popular choice for protein production. AAV and lentiviruses can be produced...More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources References Shaw, ...cell line as a platform to produce recombinant proteins and viral vectors. Frontiers in Bioengineering...