We narrowed to 724 results for: rel

... Porrot, F., Bohl, D., Marmé, F., Chérel, Y., Salvetti, A., Hurtrel, B., Heard, J.-M., Rivière, Y., & ...your specific experimental system, rather than relying on other systems as proxies.  Finally, the size...consider. First, the titer required is inversely related to transduction efficacy: the higher the efficiency...against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene...

...45), there are also initiatives to create more reliable reagents for future research. I’ve talked to many...many researchers who have complaints about the unreliability of antibodies. IPI is working to address this...repeat previously published results. This over-reliance on the perfect story for a “high impact” publication...ways to publicise negative data - we don’t have to rely on traditional journals. There’s social media - ...publishing community views reproducibility, how industry relies on reproducibility, and how you can push for more...

...assemble a rough guide to the science of Ebola and related resources. Ebola Basics A few basics: Ebolavirus...outbreak and earlier ones, along with links to the relevant scientific literature.  -In light of the current...Medicine have made their complete collection of relevant papers freely available here. -PLoS and The New...of Medicine have similarly curated their Ebola-related content. -And the latest updates on the ongoing...

...attracting and keeping diverse talent in science related careers. Failure to strengthen the talent pipeline...project and the findings were captured in this just released report “Enhancing Dissemination of Evidence-Based... join us for the pd|hub Workshop Report Public Release Event scheduled for March 4th from 2:30-4:00 pm...science career resources Browse all blog posts related to science careers Download the Science Career ...

.... During this time, scientists discovered many related immune mechanisms that could target foreign DNA... effectively. The current classification system relies on a combination of sequence similarity, phylogenetic...same name but be completely different! They are merely linked by a similar function. After analysis, CRISPR...various functions. Class 2 Class 2 CRISPR systems rely on a single protein Cas effector and are likely ...broken down into type II, type V, and type VI, each relying on a unique Cas effector — Cas9, Cas12, or Cas13...

...called a Glycosylase Base Editor, or GBE, for its reliance on the glycosylase UNG to crease abasic sites ...hypothesized that the CGBE edit process occurs entirely while Cas9 is bound, keeping the two DNA strands...regions that were already accessible. It's not entirely clear why this is, but it might point to a more...: CGBEs and indels Cytosine transversion editors rely on a multi-step cellular repair pathway that is ... GBE Glycosylase Base Editor A base editor that relies on DNA glycosylation to create an abasic site. ...

...often happen when we allow ourselves moments of release and mental freedom. Practical approaches Engaging...to detach from the constant stream of thoughts related to lab research and gain fresh perspectives. Such...encourages exploration of tangents or seemingly unrelated ideas. It's beneficial to carry a notebook at ... identify areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives...

... of the time because of relationships. It’s much harder to build relationships when you are more desperate...experiences to share. The conversation builds a relationship for the future and the 1:1 format is good for...you will make good progress on both building relationships and thinking about future career options. 5....

...access to coronavirus-related plasmids and to receive important coronavirus-related deposits. Our valiant...daily operations for all ongoing processes are entirely self-supporting. We hire more staff as we need...vector distribution is recovering from the pandemic-related slowdown. More scientists are sharing plasmids ...

...requires travel can show that you are self organised, reliable, and can work independently. Practical outdoor...are highly desired, as roles are becoming more reliant on technology, and good computer skills will give... every role you need to show that you have the relevant skills and experience.You might not normally think...content in your Resume and Cover Letter. Include any relevant job specific ‘buzz words’ to ensure recruitment...

...site-specifically to alter its function, mimic a biologically relevant modification, or add in fluorescent or chemically...which can help determine structure and function relationships or, in more practical applications, allow a ...tRNA, and the tRNA synthetases. And, naturally, rarely are the optimization parameters the same for all... any of a huge variety of ncAAs to study the relationship between structure and function…if you can get...

...participants in skills useful for future mentoring relationships.   If you would like to start a group mentoring...for the time commitment of a formal mentoring relationship or there aren’t enough senior mentors available...ideas on what to do once you are in a mentoring relationship.  Celebrate your successes and support each... come. Are you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring...

...resulting in the activation of keratinocytes, which release inflammatory mediators that trigger the activation...pharmaceutical interventions that would inhibit IL-36 release,” says Dr. Mark Mellett, a senior researcher at...autoinflammatory loop: keratinocytes both respond to and release IL-36 cytokines. Keratinocytes are the main source...what molecules or proteins are involved in IL-36 release in keratinocytes,” says Mellett. This would help...

...This biosensor changes fluorescence based on the release of glutamate from neurons and this new version ...iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at once. “This work has...functional contribution to a variety of behaviors related to addiction and pain. Once these are completed...neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine administration and...

...into larger units or circuits with functional relevance following a set hierarchy. MoClo originally developed...technique. Though rarer, you can find MoClo that relies on Gibson and other non-Golden Gate cloning methods...libraries depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed...identify the best cocktail of genes when the relationship between genotype and phenotype is best described...

...neuronal firing. What is chemogenetics? Chemogenetics relies on genetically introducing engineered receptors... However, concerns have been raised over the reliability of using CNO as a DREADD agonist due to its ability...the compound, or compounds, you choose to ensure reliable results (Goutaudier et al., 2019). Using PSAMs...BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated...

...embark on or continue their careers in science-related fields. In particular, two recent events have the...note is a ruling in August by the National Labor Relations Board to allow graduate students who work as teaching...university and alterations to the student-university relationship, along with, of course, union dues. Time will...institutional responses to the FLSA ruling. Similarly, from purely a salary perspective, while this is a move in the...

...of an outbreak of the virus in Brazil that was correlated with greater rates of infection and rare, but...develop our paper-based platform into clinically relevant diagnostics for the Zika virus was daunting. It...Our approach now detects samples at clinically relevant concentrations (1-3 fM; 1x106-fold lower than ...shown that we can detect the virus in a clinically relevant setting. Molecular diagnostics for the Zika virus...

... (such as a gene) and initiate the process of releasing the newly synthesized RNA from the transcription... is also known as intrinsic termination, and it relies on the formation of a GC-rich hairpin in the RNA...terminates differently. Polymerase III, for example, relies on a specific sequence and RNA secondary structure...downstream GU-rich region (shown in the figure below) releases the mRNA from the polymerase and creates a free...

... activation, and knockout. Most CRISPR screens rely on lentiviral systems, a fact supported by the wide...still beats lentivirus. In mice, AAV is almost entirely non-immunogenic — meaning when conducting in vivo...not integrate into the host genome. AAV instead relies mainly on episomal expression. Episomes are circular...It is important to note, however, that while AAV rarely integrates into the genome, CRISPR may increase...