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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 323 results for: sars

Showing: 301 - 320 of 323 results

  1. Antibodies 101: Validation

    Type
    Blog Post
    ...: validate, validate, validate. This doesn’t necessarily mean that you yourself have to perform extensive...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... human U6 promoter to drive expression, is it necessary to add a G nucleotide to the start of my target...

  14. CRISPR Guide

    Type
    Guide
    ...detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar to Cas9 and...Plan Your Experiment Web References PAM Sequences Glossary Publications CRISPR Overview Bacteria have an ...synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20-nucleotide...collection are from S. pyogenes unless otherwise noted. Glossary Term Definition Base editor Fusion of a Cas protein...Sequence adjacent to the target sequence that is necessary for Cas enzymes to bind target DNA PCR P olymerase...551 (7681), 464–471. PMID: 29160308 Hess, G. T., Frésard, L., Han, K., Lee, C. H., Li, A., Cimprich, K. ...L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J...

  15. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... Common Uses Safety Resources Plasmid Elements Glossary Gamma-retroviruses are a subtype of retrovirus...types include murine leukemia virus (MLV), murine sarcoma virus (MSV), and feline leukemia virus (FeLV). ...vectors or simply need a refresher, we've included a glossary at the end! Browse retroviral plasmids available...Retroviruses have simpler genomes, containing only the necessary packaging genes, while lentiviruses also contain...packaging genes and the long-terminal repeats (LTRs) necessary for integration. Wildtype gamma-retroviruses contain... contain the gag , pol , and env genes necessary for gamma-retroviral production, and scientists have...containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning and working with...

  16. Lentiviral Vector Guide

    Type
    Guide
    ...long-terminal repeats (LTRs) necessary for integration. Not all of these components are necessary for lentiviral production... Common Uses Safety Resources Plasmid Elements Glossary Lentiviruses are a type of retrovirus and belong...vectors or simply need a refresher, we've included a glossary at the end! Browse lentiviral plasmids available...by using three separate plasmids encoding the necessary viral genes Safer; replication incompetent by ...containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning and working with... viral production followed by transduction is necessary to properly utilize lentiviral vectors. Some lentiviral...provirus. The U3 (unique 3’) contains sequences necessary for activation of viral genomic RNA transcription...

  17. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Common Uses Safety Resources Plasmid Elements Glossary Adeno-Associated Viruses (AAV) are small, non-...vectors or simply need a refresher, we've included a glossary at the end! Browse AAV plasmids available at Addgene...plasmid) — containing the Rep and Cap regions necessary for the production and assembly of viral capsids...containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning and working with...efficient AAV replication as it activates the necessary cellular machinery to produce large amounts of... for infection and to express a viral element necessary for RABV trans-synaptic transport. Learn more ...translation and inhibiting cellular anti-viral defenses. Glossary Term Definition Adeno-associated virus (AAV) Wildtype...

  18. Optogenetics Guide

    Type
    Guide
    ...domains used in these plasmids, see the Glossary below. Glossary of Optical Switches Category Variant Description...Example: eMac3.0 Browse Leptosphaeria plasmids . Glossary of Microbial Opsin Variants Opsin Type Variant...

  19. Sequencing Primers

    Type
    Guide
    ... Forward GCTCGATACAATAAACGCC (Invitrogen) Rous sarcoma virus (RSV) promoter, forward primer pRS-marker...ACATGGGTGGTGGTATAGCGCTTGCG (Orsulic lab) 3' of Rous sarcoma virus (RSV) env gene, forward primer Rluc-F CCAGGATTCTTTTCCAATGC...

  20. Cloning

    Type
    Guide
    ...in the annealed product, ligation is rendered unnecessary. The product may be transformed directly into...
Showing: 301 - 320 of 323 results