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TypeBlog Post...why the results you've measured should change established theory. So, what can you do to ensure that your...available. But think of this as a jumpstart or a refresher for your skillset rather than a permanent fix....
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Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...Scientists Using Social Media" tip sheet from Science Media Savvy This tip sheet provides advice for tweeting... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...homologue) or extensive DNA synthesis. NHEJ also finishes repair of most types of breaks in tens of minutes...Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683... -
Protocol - How to Create a Bacterial Glycerol Stock
TypeProtocol...-80°C. Subsequent freeze and thaw cycles reduce shelf life. To recover bacteria from your glycerol stock...it from thawing completely and will improve the shelf life. It is very important that you shake the glycerol... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following... -
RNA Extraction Without A Kit
TypeBlog Post... kits to make their life easier. Recently, we published a blog on DNA purification without a kit that ...molecules (Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater... -
Human Germline Editing Using CRISPR
TypeBlog Post...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...with a storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...self-assembling protein (SAP) such as a bacteriophage shell protein or a bacterial microcompartment protein....expressing the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent... -
Working with Nuclear Receptors
TypeBlog Post...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...the basic assay remains widely used today. The sheer abundance of these critical research tools is a ... -
A Look at Addgene's QC Process
TypeBlog Post...across the globe. This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli... Alyssa Shepard... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...involves preparation of the tissue sample, several washes, and temperature changes to visualize the expression..., is available from Addgene. If you use these published protocols and want to share pictures, feedback... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...dCas9, also referred to as CRISPRa, was initially published in 2013 (Bikard et al., 2013, Perez-Pinera et ..., Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying W, Zhang J, Tang C,... -
CRISPR in the Clinic
TypeBlog Post...expanded organoids. This work, and many others, pushed the field forward to where it is today. Below we...because of its potential for allogenic “off-the-shelf” treatment. In short, instead of using the patient... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...of growing collections of music. I still have a shelf full of CDs that have been gathering dust since ...tested for antigen binding. If starting from an established hybridoma line, the antibody can be converted... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...spacing between them so that they are easily distinguished. The UCLA Library offers some Powerpoint based... -
Hot Plasmids February 2024
TypeBlog Post...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...-3188559/v1. Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance ... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post... is fast and can cut in as little as 2 hours. Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org...Expanded serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...landscape facing all scientists, both young and established, as they embark on or continue their careers .... and Social Media Content Specialist at Thermo Fisher Scientific Developing effective science advocates...