... to target multiple delivery systems, such as lentivirus and MMLV gag, to specific cell types. It can ...

...floating. In the same paper, the authors used lentivirus to introduce an shRNA to downregulate expression...

...replication machinery of Kaposi’s sarcoma-associated herpesvirus.       References 1. Snapp, Erik. "Design and...

...constructs were designed to be packaged into a retrovirus for infection of host cells. The fluorescent ...

... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

...the plasmids must first be used to make virus. This pooled virus is subsequently used to deliver the plasmids...electroporation and maxiprep). If delivering as virus, make virus; this creates a pooled lentiviral CRISPR library...

...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...

...our CRISPR guide pages Find ready-to-use Cas9 lentivirus ...

...types of viruses that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) ...) Adenovirus γ-Retrovirus Read More...

...analyses to pinpoint genes linked to parasite virulence, transmission, and development. This collection...

...Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine...

...upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing...

...recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 gRNAs. Frew pSQT1313...

...applications in molecular biology, plasmid cloning, and virus, including: Gel Electrophoresis Bacterial Transformation...

...mouse models. For viral injection, Adeno-associated virus (AAV) is a widely-used tool for achieving in vivo... especially useful in AAV experiments where the virus can spread around the injection site and potentially...

...localized expression of the opsin. Depending on the virus and promoter system used, there is an incubation...Sabatini BL 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression...

... be efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9...editing method in which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the...H840A nickase and M-MLV RT (Moloney Murine Leukemia Virus Reverse Transcriptase) for "search and replace" ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...

...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ...

...Constitutive Strong mammalian promoter from human cytomegalovirus EF1a Constituitve Strong mammalian promoter...