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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 728 results for: SON

Showing: 161 - 170 of 728 results

  1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...your next project. Gymnotiformes and Mormyridae (Jason Gallant) Weakly electric fish are excellent systems... 60 °C soils. Dendrobatidae (Lauren O’Connell)  Poison frogs are great for studying parenting strategies...generate cardenolides that tastes yucky (and is poisonous) for bird predators - birds eat a caterpillar ...of a pigmentation gene.   Genus Microtus (Zoe Donaldson) Voles have fascinating social behavior. For example...Claudio Mello) These very cute birds learn their songs like humans learn speech. Finches must learn early...system to rapidly identify recombinants. Arthropods (Jason Rasgon) This lab is developing tools for pan-arthropod...

  2. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... make sure to get that person’s contact information and again, make a personal connection! Even if you...does, however, offer you an opportunity to make a personal connection; the importance of which will be elaborated...first for companies at which you’ve already made a personal connection. That way, once you’ve identified an...email with the relevant info and they just might personally hand your credentials to HR, greatly increasing...information and establish a connection with that person before ever asking them for work. Addgene’s very...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...difficult to find solutions to. There are many reasons why client situations might be especially challenging...skills is a must to be successful in this field. Personally, I have greatly benefited from and appreciated...when their teams are facing a crunch, and using lessons from previous cases to better staff future teams...conflicting work styles, team situations, and personalities. Teams striving to achieve a lot of great output...coach and counsel employees on the different personality and work styles (e.g., Myers-Briggs). Staff are...teams, to leverage the preferences of different personality types, and to devise strategies to be prepared...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    Interesting...now well-known FRET, aka Fluorescence (Förster) Resonance Energy Transfer, technique which allows the detection...have any cross-talk but you also don’t have any resonance transfer. You have to find a balance between FRET...downloads/modules/FRET_teaching_module.pdf Day RN, Davidson MW. Fluorescent proteins for FRET microscopy: ....201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET) microscopy imaging of live...

  5. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...the question of how much data was necessary and reasonable. PLOS has since clarified their data sharing ... in having exclusive access to data and enjoy reasonable time to make sense of it, with their decision...The real cost of making data accessible A more reasonable concern with the data policy was raised by researchers...how results were obtained and this alone is a reasonable motivation for increasing both data accessibility...published data. The importance of data sharing The reason this topic is important to my lab is three-fold... welcomed. However, expectations must also be reasonable, sensitive to the data type and made as facile...

  6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...with Gibson assembly. While this is a comprehensive mutagenesis method, a drawback to using Gibson assembly...plasmids ready for deep mutational scanning.     3 reasons to be interested in deep mutational scanning Hundreds...rate of mutagenesis, and low throughput.       Gibson Assembly: An easy-to-use technique that joins homologous...especially single basepair deletions. Therefore, Gibson assembly is better suited for creating small, focused... PubMed PMID: 27723752.  4. Fowler, Douglas M., Jason J. Stephany, and Stanley Fields. “Measuring the ...

  7. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...2015b) under a CC-BY 4.0 license. Long et al., Nelson et al., and Tabebordbar et al. each chose to try.... used SpCas9 in conjunction with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter ...not subject to nonsense-mediated decay. Although Nelson et al. observed only 2% genome editing in one experiment...persist long-term. In accordance with this result, Nelson et al. find that dystrophin restoration is maintained... E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome editing partially restores ...Dec 31. pii: aad5725. PubMed PMID: 26721683. 3. Nelson CE, Hakim CH, Ousterout DG, Thakore PI, Moreb EA...

  8. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ...path. Because there is no demand for a favor, the person you ask will be more likely to say yes and more... Informational Interviews are best conducted in person. Perhaps you can offer to buy coffee or lunch at... your interviewee? It is always best to meet in person to develop relationships, but if it is not possible.... Keep in mind that this is a good time to ask personal questions about salary, opportunities for advancement...advancement, challenges and disappointments. These more personal questions can elicit the most valuable insights...

  9. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...secrets - during a collaboration, members of Maureen Hanson’s and Rima Menassa's labs made the accidental discovery...created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the unexpectedly...Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. Green to red photoconversion of GFP for protein... PubMed. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible...from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV...

  10. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...and Hoess, 1984; Van Duyne, 2001; Christenson Wick and Krook-Magnuson, 2018). Optogenetic FLEx vectors ...PubMed PMID: 11340053. Wick, Zoé Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and...Nature 351.6326 (1991): 489. Hirsch, Matthew L., Sonya J. Wolf, and R. J. Samulski. "Delivering transgenic...PMCID: PMC5111696. Towne, Chris, and Kimberly R. Thompson. "Overview on research and clinical applications...
Showing: 161 - 170 of 728 results