...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for ...zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (...different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can...

...until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise changes in histone... Cindy Y. Jao, and Alice Y. Ting. "Genetically encoded fluorescent reporters of histone methylation in...Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors...grandsons that decreased their likelihood of death from cardiovascular disease but increased their risk of... DNA methylation patterns, either large increases or decreases. Hypermethylation of CpG islands in the... units of the FRET pair together and causes an increase in the FRET signal. Subsequent demethylation separates...

...and general DNA repair Reduces plasmid recombination, increases plasmid stability recBCD Exonuclease...mutation Improves plasmid yield F Host does (F') or does not (F-) contain the fertility plasmid. A low copy-number...copy-number plasmid, encodes proteins needed for bacterial conjugation. Genes listed on F´ are wild-type...Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page!     ...make major improvements such as those that increase plasmid yield and/or DNA quality. Table 1 below outlines...Exonuclease V activity abolished Increased plasmid stability, reduced recombination relA or relA1 Relaxed phenotype...General cloning and storage of common plasmids, blue/white screening. F- endA1 glnV44 thi-1 recA1 relA1 ...

...efficiency of plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and... histidine at amino acid 66. This histidine is encoded by a ‘CAC’ codon that is converted to a ‘TAC’ or...antibiotic resistance markers, and other enzymes such as Cre-recombinase drastically expanding the base-editing...Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...Base editors create specific point mutations in the genome, but they’re inefficient compared to CRISPR...experiments. In the past few years, scientists have created an array of base editing reporters that can help...Cas9-mediated editing in real time The Harris lab created ACE, a reporter that monitors APOBEC (a family ...

... Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety (see slides...is in one of the top 10 countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic...transcriptome sequencing methods including work done by the ENCODE project consortium did not do a good job of seeing...resulted in a cell migration defect. They have created a circRNA website so scientists can view public...developments. Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system....

...HDR efficiency with circular plasmid templates is generally low; to increase the frequency of edits, researchers...of a homologous donor (e.g., sister chromatid, plasmid, etc.), and consists of three pathways: classical...disrupted in the donor. Choosing a type of donor: plasmid, oligo, double or single stranded? The size of ...longer than 200 bases can be difficult, thus dsDNA plasmids are generally preferred for large insertions such...researchers have designed self-cleaving plasmids that liberate the targeting region from the vector. Alternatively...these are generally more toxic to cells than their plasmid counterparts. Another consideration for dsDNA templates...., 2017). Easi-CRISPR in vitro transcribes RNA encoding your repair template, then uses reverse transcriptase...

... resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101 Biotinylation Other resources...vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and... specific locations in the cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate peroxidase... They named this mutant APEX2.  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity...allowing for more precise temporal control.  Find plasmids for TurboID and miniturbo Split-BioID: A conditional...streptavidin and separated via flow cytometry.  Find plasmids for EXCELL here! Since BioID was developed in ...Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature biotechnology...

...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene   ...this approach can greatly increase the sensitivity of an assay by increasing the number of signaling molecules...green; and proteins are represented in orange. Created with BioRender.com.    How do secondary antibodies...shorter than that of the primary antibody, due to increased binding affinity, many indirect antibody protocols...are designed to take place over multiple days. Increased run time is considered one of the major drawbacks...specific isotype subclasses of primary antibodies can increase the possible number of target proteins.   The ...immunohistochemistry advances have found ways to increase signals from conjugated primary antibodies. The...

...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...simple as it seems. Take, for instance, the GCaMP-encoding viral vectors available in the repository. GCaMP...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...

...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics...basic guidelines below, you’ll likely be able to create a small, usable panel.  First, fluorophores vary...