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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 643 results for: ATO

Showing: 321 - 340 of 643 results

  1. Hot Plasmids - November 2023

    Type
    Blog Post
    ...example, neurons treated with a phorbol ester activator gave fluorescent signal in the ticker tape from...transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

  3. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first...Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389...

  4. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...

  5. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...own replication machinery such as Rep or other initiator proteins, they do not require host proteins for...

  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...

  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...

  10. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...line and ~8 and 9% efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers ...

  12. March for Science

    Type
    Blog Post
    ...know is your area code. Senators: To find contact information for your senators all you need to do is select...

  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

  14. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...Often, however, researchers lack knowledge of the regulatory kinase, access to active kinase for the reaction...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

  16. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...academic publishing goals and make you a better communicator overall. Don’t Write in a Vacuum Your research...through many iterations read by your PI, your collaborators, others you know in the field, and even people...

  17. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would...

  18. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

  20. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...
Showing: 321 - 340 of 643 results