...xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7 fused to third generation cytidine ...

...PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right...

...repair harmful double stranded breaks, in which nucleotide sequences are exchanged between two similar or...

...traversing pons and medulla, bp= basilar pons, NST nucleus of the solitary tract.  The arrows point the direction...

... from transgenes to plants, chloroplast to the nucleus, or pathogens to their hosts. I spoke with her ...

...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

... cloning by homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID:...

...because they remain in episomal form in the cell’s nucleus. But since nothing’s ever perfect, neither is ...

...with SRM techniques, and adapted for detecting nucleic acids as well as proteins (Klimas, et al. 2023;...

...African descent have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the...

...fluorophores were designed as antibody and oligonucleotide labels for fixed-cell imaging, not for live-cell...

...think of DNA as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong....

...activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope...

...in 5). The RNA genome is encapsidated by the nucleoprotein, NP. NP associates with the viral polymerase...

...cell type, even within the same brain region or nucleus. Just optimizing AAV expression can take months...

... with a tag that localizes your protein to the nucleus? Or you want multiple rounds of purification to...

...retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing ...

... represent all 4096 (4 6 ) combinations of 6 nucleotides. Chong Zhang E. coli Genome-wide Inhibition Library...Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout ...

...improves transduction efficiency by facilitating nuclear import of the vector’s preintegration complex in...found, search for NAR(N 17 )YNN, where N is any nucleotide, R is a purine (A,G), and Y is a pyrimidine (...targeting introns. Avoid stretches of 4 or more nucleotide repeats, especially repeated Ts because polyT...program. Select sequences that have at least 3 nucleotide mismatches to all unrelated genes. TIP: Addgene...

...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...