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Showing: 481 - 510 of 1076 results

  1. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers blog articles, ... our visitors made sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory...teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very popular, with...

  2. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... take into account chromatin context and other factors, they are easy to use and can reduce off-targets...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...- GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would... (e.g. in a gene in a similar pathway, a growth factor, etc.) this control is usually not very meaningful...and off-target risks will vary depending on many factors. Hopefully now you have all the resources you need...

  3. Finding Your Perfect Job After University

    Type
    Blog Post
    ...and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic that ...and the pro’s and con’s of working in different sectors. Luckily, I have worked for a variety of different...options for going into parasitology but another major factor held me back; I do not have a second language. ...allowed me to try out working in many different sectors and to discover for myself what potential job opportunities... Are Open at Addgene Learn How to Choose a Good Mentor for Your Professional Development ...

  4. Science Communication for Everyone

    Type
    Blog Post
    ...Chinmaya Sadangi, a postdoc at the University of Toronto. The Addictive Brain was founded in early 2018 ...Chinmaya Sadangi, a postdoc at the University of Toronto, created The Addictive Brain to spread awareness...non-scientists can directly approach scientists and doctors with their questions and comments.  Lastly, we ...Keep your message to the main three points of your story. Interested in learning more? Visit their website...Sadangi is currently a postdoc at the University of Toronto and founder of The Addictive Brain. Prior to this...

  5. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...signaling activity and cell phenotype. One method for monitoring live single-cell kinase activity is FRET, but... distance and the orientation of the donor and acceptor proteins, which can be difficult to predict. ...disadvantages when compared to FRET reporters. First, KTRs monitor global kinase activity that occurs in the cytoplasm...experiments. Third, KTRs are not well suited for monitoring kinase activity that occurs during nuclear envelope...the nucleus, there’s no nuclear localization to monitor). Whether you’re looking for a ready-to-use KTRs...

  6. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...here!   Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School ...Cas9) hematopoietic stem cells with a lentiviral vector containing a gRNA. These transduced stem cells ...custom mouse strains and lentiviral expression vectors (Table 1). The four systems Name Function...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

  7. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...The first is the study of non-coding regulatory elements. Regulatory DNA function is typically inferred ...interested in exploring the function of non-coding regulatory elements in their native chromatin context. An...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...PhD student at Harvard Medical School in the laboratories of Stuart Orkin and Daniel Bauer. He received...

  8. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ... successful, internationally renowned plasmid repository that it is today. Although I wish I could take... attributed to the entire Addgene family; the depositors and requesting scientists, all of the Addgene..., Addgene supporters, and of course, Executive Director, Joanne Kamens, and founders, Melina Fan, Ken ...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first). After the speeches,...

  9. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...wanted users to be able to find plasmids and viral vector tools more easily. In the redesign, the new search...unique: community, sharing, and fun. The Addgene repository is built on the foundation of a community that...dictate the space. We wanted to comfortably tell our story, to get scientists excited about what Addgene has...redesigning the homepage, and striving to tell our story in better ways, we realized that Blugene needed ...you come to Addgene to find the plasmid or viral vector for your next experiment, that you can find what...

  10. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs at UNC, called her presentation...current postdocs will be academic principal investigators. She also pointed out promising new practices...increasing the value and priority put on good mentoring and applauded the new requirement for PIs to include...opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments were about ...

  11. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ... markers all tagged with mEmerald. Plasmids to monitor protein-protein interactions through multicolor... Baird said. “They are all designed in the same vector backbone with very similar restriction enzymes....Michael Davidson told the Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer...shutter its doors. “We wanted to give all of these vectors to the scientific community and contribute in a...community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael Davidson...

  12. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi is unmatched—unlike...cannot be used to investigate essential genes. Historically, gene silencing methods were thought to be afflicted...considerations, such as the starting material (DNA-based vector or purified RNA) and the initial state of the dsRNA... Although downstream work is required, several vectors can be found at Addgene that could serve as an ...an excellent start (#22988, #137884). Both vectors allow the creation of amiRNAs based upon miRNA precursors...method. Instead of using a fluorescent output to monitor RNAi, we engineered a positive selection reporter...

  13. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...chance vs. the factor that is being experimentally manipulated. Below are several core factors of statistics...variables – factors which are not affected by other variables (cause). Dependent variables – factors which...total crop production and the production window. Factorial ANOVA This type of ANOVA assesses multiple independent...sounds like a two-way ANOVA. And this time, it is! Factorial ANOVAs include two-way ANOVAs but can include ...

  14. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It’s important to remember that because...Maria Cuervo, and Per O. Seglen. "Methods for monitoring autophagy from yeast to human." Autophagy 3.3...PMC4517063. Yoshii, Saori R., and Noboru Mizushima. "Monitoring and measuring autophagy." International journal...

  15. RUBY-Red Siliques

    Type
    Blog Post
    ...Willoughby   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al...., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic...Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors...

  16. What the HEK?

    Type
    Blog Post
    ...model for cancer-specific research. Membrane receptors and ion channels are often exogenously expressed... degrade over time in culture. This can affect factors that could skew research results (gene expression... Especially if your research interests include receptor biology, ion channels, or involve human protein...Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI...10 Tips for Mammalian Cell Culture Adenoviral Vector Production and Troubleshooting Additional resources...

  17. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...different story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start...spent your life making connections and finding mentors -- well, it's time you call upon these people for... is more important than the product itself -- investors would rather fund excellent people with mediocre...Harvard/MIT faculty, venture capital and angel investors, and business development heads at major companies...

  18. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of... have low knock-in efficiency. Together, these factors prevent stem cell researchers from generating clonal...purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure...experiments. Notably, we could tag >60 transcription factors in a short (3-week) time window; this was possible...csRNP experiments, it can be ordered in bulk and stored in small aliquots at -80 oC. Assemble csRNP: Anneal...success of knock-in experiments depends on many factors. Some of these include: Most importantly, the...

  19. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...one are in a pLVX vector background, so you can package them into lentiviral vectors if you need to. Additionally...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...It’s also important to determine if the human interactors we found in our map have an impact on virus replication... replication. While we and our collaborators are starting these experiments, there's so much to do that...

  20. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ...looping factors developed thus far for these latter experiments, such as transcription factors tethered...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal degradation of ...neurons. Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven...

  21. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...researchers, it can expose them to a new institute and mentor that they may want to consider for future work ...professor or institute, ask if you will have a bench mentor or anyone else available to directly help you during... during your visit. Reach out to your bench mentor (if you have one) to communicate your plan as well.... comfortable initiating this conversation, your mentor can help get the ball rolling! If you are applying...techniques. Show up on time (don’t make your bench mentor wait on you!), make sure you are prepared (read...

  22. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...elicit an immune response from the body. Several factors contribute to the immunogenicity of a drug, including...these algorithms to model individually-specific factors, such as previous exposures to that specific antigen...assessments, as well as analyze the contributing factors that in silico prediction algorithms fail to capture...10.1634/theoncologist.2016-0061WHO. International monitoring of adverse reactions to drugs: adverse reaction...WHO Collaborating Centre for International Drug Monitoring, Uppsala, Sweden, 1992. Mattei, A. E., Gutierrez...

  23. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...will choose to start their own academic research laboratory. How does one even go about starting a lab? Obviously... does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager, researcher, adviser...experiments and grant writing. You have to enjoy the mentoring role.” She points out that new PIs may not recognize...student or postdoc may require different types of mentoring. Some mentees prefer lots of advice and micromanagement...tips for starting a lab. Or share your success stories below. And don't forget to stay tuned for the last...

  24. Antibodies 101: Isotypes

    Type
    Blog Post
    ... are three main pathogen clearing functions or effector functions of the constant regions of antibodies...functions: Recruitment of immune cells that express receptors that recognize the Fc portion of different antibody...domains, or Fc region, can be bound by special receptors that transport antibodies through cells and into...role in allergies. They are found bound to IgE receptors on the surface of basophils and mast cells, two...of an immune response since it also changes the effector function of the antibody. Isotype switching is...

  25. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...for you to find the plasmids you need. We'll be monitoring and improving the maps further after this initial...SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier...all available sequence information from Addgene depositors and our own sequencing results: On this page ...sequence in your sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

  26. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989...be individually optimized for each of the three factors: the ncAA, the tRNA, and the tRNA synthetases. ...balance can be extremely tricky to find: each of the factors can negatively impact both translation and fidelity...complicated.” And Ryan Mehl would know. He’s the director of GCE4All, an NIH-funded center located at Oregon... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

  27. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...professional network such as co-workers, professors, and mentors. Ask for specific guidance on grammatical errors...notice within 24 hrs, send a note to the program coordinator.   6. Virtual interview prep Prepare for your...practice a set of common questions with a peer or mentor. Several days prior to the interview, inspect your...Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard...

  28. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...biological systems. Size is also a factor; the pol lll promoter and terminator need be encoded only once, and...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...gRNAs that can be packaged together in a single vector. In addition, the pol III promoter is relatively...deletions of other regions, such as noncoding RNAs or regulatory elements, to examine their function. Bacteria...

  29. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous... artificial intelligence," says Michael Levin, Director of the Allen Discovery Center. The future of this...Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (...(2018): 134-144. PubMed PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction ...

  30. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...tips can help:  If there’s a teacher, mentor, or other educator working with your audience, ask! They ...refers to the work put into creating a permanent store of knowledge (a schema).”  In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information...knowledge and expertise your audience has is a major factor in how quickly they can understand and process ...
Showing: 481 - 510 of 1076 results