We narrowed to 643 results for: ato

1. Antibodies 101: Affinity Reagents

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   Blog Post

   ...antibody constant region. They are generated using combinatorial libraries, and high specificity has been reported...

2. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...and were presented at the ASPB meeting by the collaborators who could participate. Despite the group not...

3. Addgene: The First Twenty Years

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   ...amazing years in front of us. So in our last celebratory moment, we wanted to walk through some of our...

4. Antibodies 101: Fab Fragments

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   ...secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ...

5. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...of IPTG. Because RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid...

6. RaPID Detection of RNA-protein Interactions

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   ...one to study RNAs expressed from their native regulatory elements. Controls for RaPID experiments RaPID...

7. dTAG - You're it!

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   ...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...

8. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...the Hell lab designed their super-resolution pH indicators (SRpHi) biosensors to meet all of them. These...

9. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   ...storytelling is an impactful tool for science communicators. “The power of a story is to make a human connection...

10. Viral Vectors 101: An Introduction to AAV

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    ...make AAV ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression...

11. To Each HIS Own

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    ... isolated from a complex mix using affinity chromatography against an amino acid sequence, or tag, on ...

12. Human Germline Editing Using CRISPR

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    ...proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals...

13. Lighting Up Cell Signaling with Photoswitchable Kinases

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    ...endogenous ERK, and its localization serves as an indicator of MEK1 activity. When MEK1 is active, it phosphorylates...

14. Antibodies 101: Conjugation

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    ...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...

15. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ...

16. Transferable Skills Guide: Cross-team Communication

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    ... a lot on context. New people in the lab or collaborators will not have the context you do, which means...

17. Addgene's Tips for Plasmid Quality Control

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    ... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...

18. Plasmids 101: Golden Gate Cloning

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    ...for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the correct order in...

19. Career Coaching for Scientists: Why and Where Do I Find One?

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    ...help you. A good coach usually will be a clear communicator and make direct suggestions for improvement ...

20. 5 Steps to Writing Grant Proposals for Grad Students

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    ...first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what...

21. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ...Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical...

22. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells. ABE8 efficiently edited the fetal...

23. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    ... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...

24. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...general consideration for behavioral testing in a laboratory, a high level of experimental control is required...

25. Plasmids 101: The Wide World of Natural Plasmids

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    ...Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer...

26. Making CRISPR Plasmids Using Fragmid

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    ...Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site...

27. Engaging with science and society at pgEd

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    ... in genetics research, their applications and regulatory policies; keeping tabs on the conversation about...

28. Overcoming the Challenges of Lentiviral Production

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    ...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...

29. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...Destination vectors. These vectors can induce combinatorial constitutive or inducible gene overexpression...

30. Adenoviral Vector Production and Troubleshooting

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    ...medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes, AdVs cannot...