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We narrowed to 1,014 results for: REN;

Showing: 801 - 850 of 1014 results

  1. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...research. Listen to learn more about Dr. Kwapata’s current work and thoughts on the need for more scientific...biotech research in Malawi 11:41-14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44...

  2. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...the calculator in the comments section below. References 1. Choi, Vivian W., et al. "Production of Recombinant...Adeno‐Associated Viral Vectors for In Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed...

  3. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ... Cervical cancer screening in Ghana Gladys is currently working to enhance cervical cancer screening in...screening for HR HPV alongside the cytological tests currently in use. Gladys will use plasmids containing a ...

  4. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...repository to be packaged in your choice of five different serotypes.   We are so excited for all the ways... The AAV Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV...

  5. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...activated T cells, precluding analyses of T cell differentiation beginning with naïve T cells. The Framework...improving CD8+ T cell responses to cancer.   References and resources Milling, L. E., Markson, S. C.,...

  8. Addgene's eBook Collection

    Type
    Guide
    ...you're not sure where to start or need a handy reference, Addgene's eBooks are here to help!...you're not sure where to start or need a handy reference, Addgene's eBooks are here to help! 0 of 0 Selected...

  9. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...sustainability problem, how can one little change make a difference? Individual changes to our behavior can lead ...energy, so even one less run a week makes a big difference. Clean out freezers so you can make room and ...

  10. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...detailing Nobel Prize-winning research typically weren’t among the most cited. And, as scientific discourse...made of. Increasingly, journal publications alone aren’t enough. Scientists are sharing what they call “...

  11. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...virus works its way into immune cells in ways that aren’t fully understood. Much of the action depends on...relevant scientific literature.  -In light of the current Ebola crisis, Science and Science Translational...

  12. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ... buyer to a seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does...you listed during checkout. This invoice will reference your Addgene order number and PO number that you...

  14. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small...

  16. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...hisashii, BhCas12b that is functional at 37 °C but preferentially nicks the non-target DNA strand. The lab then...approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these anti-Cas12a proteins...

  17. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...or choose to auto-approve MTAs. Whatever your preference, we are here to make it fast and easy. Fast....org or +1 617-225-9000, or check out our MTA reference pages. So go ahead and use Addgene plasmids ...

  18. Reflections and Looking Ahead

    Type
    Blog Post
    ...find what you need and to bring it in line with current Web Content Accessibility Guidelines (WCAG). These...developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende...

  19. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ...

  21. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...never clicked for me. Recently my interest was renewed in part due to FOMO  but mostly because of this.... And don’t forget to follow us @Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A ...

  23. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters the nucleus...cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete or deplete...

  26. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...costly for us to process and store materials that aren’t distributed or for our teams to keep searching,...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science...

  27. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...

  28. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging...

  29. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...potential, and who sometimes wish they could live differently than we do day to day. We discovered that it ...

  30. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are...

  31. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing...

  32. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...some our most popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR...already has five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is...

  35. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...

  36. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...likely happening in your community, and, if they aren’t, think about launching your own. Close the gap ...anyone looking to make their science matter. If you aren’t ready for a training or one isn’t available yet...

  37. Advice for Choosing a Research Project

    Type
    Blog Post
    ...research project “entails good judgement with the current technology to identify a problem, asking what approach...will I take and what can I learn, learning the difference between good and bad data, and knowing how to...

  38. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect...

  39. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...

  40. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...

  41. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...

  44. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...experimental design and usage of these tools, which aren’t always easy to find in publications, so this is...submit data. Please note that the data hub is currently still in beta version, so we’re always looking...

  46. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...

  47. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...into all cells? Schwarze SR, Hruska KA, Dowdy SF. Trends Cell Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22...

  48. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...

  49. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...provides something that you can’t obtain at your current institute. A common reason is hands-on training... right for you, start thinking outside of your current research box, send out some feelers, apply to programs...

  50. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...
Showing: 801 - 850 of 1014 results