We narrowed to 916 results for: Tro;

1. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   ...practices for reproducibility: data management plans, electronic lab notebooks, wet lab protocols, reagent sharing...

2. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   ...protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic...

3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   ...in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged...

4. Summer SciComm Series: Cognitive Load

   Type

   Blog Post

   ... audience.  More resources on the Addgene blog Intro to our Summer SciComm Series...

5. Developing Lab Management Software for Biology

   Type

   Blog Post

   ...individually keep track of any failures and do troubleshooting on their own. For a few samples this isn’t ...

6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   ...Created with BioRender.com. To decrease the electropositivity of the HNH/RuvC groove, Slaymaker et al. made...

7. New Tool for Lineage Tracing: The ClonTracer Library

   Type

   Blog Post

   ...similar to an academic research environment” with a strong focus on publishing, but still made it so she could...

8. Plasmids 101: The Wide World of Natural Plasmids

   Type

   Blog Post

   ...plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote...

9. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   ... in the hands of the Doench lab. Additionally, controls available in the system are validated and were...

10. How to Prepare for an Industry Interview

    Type

    Blog Post

    ...allow it. Before you start your presentation, introduce yourself to your audience. Pay attention to...

11. Engaging with science and society at pgEd

    Type

    Blog Post

    ...communication posts Extracurricular activities for a strong science career path Resources on Addgene.org ...

12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ... Decades ago, other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could...

13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...techniques that you can use antibodies for in our Intro to Antibodies post Resources on Addgene.org Browse...

14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    ...boiling point of water. Dr. Terns gave a great introduction to the repeated and distinct aspects of the ...

15. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    ...living cells where methylation of these sites was controlled, the reporter consistently showed significantly...

16. Hot Plasmids: Winter 2025

    Type

    Blog Post

    ...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...

17. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    ...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using...

18. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    ...little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites. Long-range...

19. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type

    Blog Post

    ...using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic...

20. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

21. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    ..., freeze them, and store all but one in liquid nitrogen. The frozen vials make up your master stock, while...

22. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    ...mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences but ...

23. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

24. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    ...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact...

25. Transferable Skills: Negotiation

    Type

    Blog Post

    ...purchases do we prioritize in a lab with both electrophysiologists and molecular biologists? What is their...

26. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    ...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo...

27. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    ... Digest Additional Resources Sigma-Aldrich Introduction to Microbial Media BitesizeBio: How to Get Better...

28. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    ...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...

29. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    ...  Susanna Stroik...

30. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    ... messy, and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the...

31. SciComm: Taking Science to Elementary and Middle Schools

    Type

    Blog Post

    ... with a good public school system that has made strong efforts in the last 5 years to incorporate more...

32. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ... requires people to be in groups, organize your troops prior to the event, and let people know exactly...

33. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models...

34. How to Be an Excellent Trainee

    Type

    Blog Post

    ... Susanna Stroik...

35. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    ...SHERLOCK had a few limitations when it was first introduced in 2017 so the lab made further modifications...

36. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    ...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

37. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ...expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...

38. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...

39. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    ...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...

40. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    ...recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban Asbestos Now Act of 2016...

41. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    ...onto data longer before publishing. But there are strong incentives to publish such as precedence, recognition...

42. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    ...Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...

43. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    ...2018). NIR FPs can be combined with blue-light controlled optogenetics, such as channelrhodopsins and tools...

44. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    ...Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab.  As with all plasmids in the repository, we...

45. March for Science

    Type

    Blog Post

    ...Additionally, interest groups are often pandered to when strong or overlooked when small, but data is not going...

46. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    ...excessive costs in your lab. That in itself is a strong negotiating tactic, and puts you ahead of most ...

47. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant expertise and...

48. Typing CRISPR Systems

    Type

    Blog Post

    ..., well understood, and the first taught when introducing CRISPR as a technology. There are three subtypes...

49. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    ...effects. Typically the phenotype would be studied in vitro, but there is enormous potential in performing in...

50. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    ...reduction as well as progression. Dunn et al in 2002, introduced the “Three Es of Immunoediting” nomenclature....