We narrowed to 398 results for: AGA

1. Plasmids 101: Broad Host Range Plasmids

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   Blog Post

   ...RepB) necessary for replication in various hosts (Pagatto and Dillon 2001).  The plasmid has its own replication...

2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional...

3. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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   ...D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent...

4. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   ...Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral supporters Denise...

5. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...Increasing rounds of transfer on transposition-inducing agar lead to higher cargo copy numbers. (D) The effect...

6. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   ...squash" originated from the squash itself, but think again. Spaghetti squash is actually a component of the...

7. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   ...favor a different model. Finding evidence for or against a model is a standard scientific strategy. However...

8. Deciphering the Mysteries of Behavior with Viral Vectors

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   Blog Post

   ...open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents have a natural...

9. Overcoming the AAV Size Limitation for CRISPR Delivery

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   ... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune...

10. Plasmids 101: Shuttle Vectors

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    ...synthesis, and the neomycin resistance gene protects against any of them. But different aminoglycosides work...

11. CRISPR 101: Any Base Transversion Editors

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    ...other transversion editors be close at hand? Once again, this post has a lot of acronyms, so we've included...

12. Bioinformatics at Addgene

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    Blog Post

    ...QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow   Over the years we have...

13. Antibodies 101: Reading a Flow Plot

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    Blog Post

    ...different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs are CD11c...

14. Changing Labor Laws Bring Increased Postdoc Wages

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    Blog Post

    ...as of October 1st the salary minimum was raised again to $51,600, itself a raise of 3.2%. Other institutions...

15. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli...

16. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post

    ... populations with basically a smear in between. Again, it can depend on the type of stain whether a tighter...

17. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    ...αVβ3.13, may also block function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived...

18. Tips for Getting a Faculty Position

    Type

    Blog Post

    ...create a new field, impact disease treatment, etc., again in clear terms. "Implications for human disease"...

19. Hot Biosensors 2022: Year-End Roundup

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    Blog Post

    ...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded...

20. Filming Science Videos in the Age of Social Distancing

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    Blog Post

    ...what works and what doesn’t, and trying all over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn...