Addgene

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Showing: 321 - 340 of 394 results

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Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

...favor a different model. Finding evidence for or against a model is a standard scientific strategy. However...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

...Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa. Financial and moral supporters Denise...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional...
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Type

Blog Post

...D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent...
CRISPR-based Genome Editing Systems For Editing in E. coli

Type

Blog Post

...Increasing rounds of transfer on transposition-inducing agar lead to higher cargo copy numbers. (D) The effect...
Deciphering the Mysteries of Behavior with Viral Vectors

Type

Blog Post

...open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents have a natural...
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Type

Blog Post

...squash" originated from the squash itself, but think again. Spaghetti squash is actually a component of the...
Bioinformatics at Addgene

Type

Blog Post

...QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow Over the years we have...
Changing Labor Laws Bring Increased Postdoc Wages

Type

Blog Post

...as of October 1st the salary minimum was raised again to $51,600, itself a raise of 3.2%. Other institutions...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

...of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...
Antibodies 101: Introduction to Gating in Flow Cytometry

Type

Blog Post

... populations with basically a smear in between. Again, it can depend on the type of stain whether a tighter...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...αVβ3.13, may also block function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived...
Five Popular Model Organisms, Part 2

Type

Blog Post

... to be replicated. Because E. coli are easily propagated and have a generation time of around 20 minutes...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

...template that corrects the blocking mutation and again identify the right clone from a few hundred. From...
Filming Science Videos in the Age of Social Distancing

Type

Blog Post

...what works and what doesn’t, and trying all over again. Here’s that process! Figure 1: The Design-Build-Test-Learn...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent...
Tips for Getting a Faculty Position

Type

Blog Post

...create a new field, impact disease treatment, etc., again in clear terms. "Implications for human disease"...
Hot Biosensors 2022: Year-End Roundup

Type

Blog Post

...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded...

Showing: 321 - 340 of 394 results

Results per page:

Designing Your Chalk Talk for the Academic Job Interview

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Overcoming the AAV Size Limitation for CRISPR Delivery

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

CRISPR-based Genome Editing Systems For Editing in E. coli

Deciphering the Mysteries of Behavior with Viral Vectors

Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Bioinformatics at Addgene

Changing Labor Laws Bring Increased Postdoc Wages

Plasmids 101: E. coli Strains for Protein Expression

Antibodies 101: Normalization and Loading Controls for Western Blots

Antibodies 101: Introduction to Gating in Flow Cytometry

An Integrin Antibody Toolkit from IPI

Five Popular Model Organisms, Part 2

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Filming Science Videos in the Age of Social Distancing

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Tips for Getting a Faculty Position

Hot Biosensors 2022: Year-End Roundup

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