We narrowed to 444 results for: ENA

1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   ...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether...

2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   ...ccd operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic...

3. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   ...specificity, eSpCas9, SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian ...

4. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   ... of the biomedical research sector, and what a tenable workforce structure may look like. The panel discussion...

5. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   ...multiple internal restriction sites that may not be amenable to "domestication", you might want to consider...

6. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   ...where I dump all of my papers and they all have filenames (year first author name). It’s rudimentary, but...

7. Working with Nuclear Receptors

   Type

   Blog Post

   ...of all organisms depends on efficient energy maintenance - through acquisition, storage, and utilization...

8. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

   Type

   Blog Post

   ...deletion). Find the Plasmids Here Plasmid features enable multiple student experiments We used TOPO cloning...

9. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   ... Doench, J. G. (2024). Modular vector assembly enables rapid assessment of emerging CRISPR technologies...

10. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    ... stripping solution uses heat and detergent to denature and remove antibodies but is also likely to remove...

11. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    ...you can start by asking ‘What is the biological phenomena I want to observe?’ This information, along with...

12. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    ... K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot Golden Gate assemblies of unprecedented...

13. Hot Plasmids - October 2020

    Type

    Blog Post

    ...al., 2020. The researchers tested several scenarios from embryo development to myeloid proliferation...

14. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    ...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...

15. How to Prepare for an Industry Interview

    Type

    Blog Post

    ... get ready for this interview? Maybe the above scenario sounds familiar to you. It was my experience not...

16. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    ... is a new system from Jiazhang Lian’s lab that enables simultaneous gene activation, repression, and editing...

17. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    ...to use non-standard Cas9 in the ABE8 complexes enables a broader targeting scope for base editing.  Try...

18. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    ...studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer to the CNS using recombinant...

19. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    ... "The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans...

20. Hot Plasmids February 2024

    Type

    Blog Post

    ...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse...