We narrowed to 396 results for: ICL

1. Cpf1 Update: Comparison to Cas9 and NgAgo

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   Blog Post

   ...Get More Cpf1 Background Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW...

2. Plasmids 101: Terminators and PolyA signals

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   Blog Post

   ...Bacterial Transcription Terminators: The RNA 3′-End Chronicles. Journal of molecular biology. 2011. PMID: 21439297...

3. Gaining Leadership Skills Volunteering at a Professional Organization

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   Blog Post

   ...JulietAdvocSci.        Further Reading Find more Career articles on the Addgene Blog Read Joanne Kamen's Management...

4. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ...where her research is focused on engineering nanoparticles as drug delivery systems. She is also a NASA...

5. Hot Plasmids: Winter 2025

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   Blog Post

   ...preps in the repository through our Hot Plasmids articles.  Here's what you'll find in this post: Optimized...

6. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ...there are many cases where the specific viral particles you need are not available, or the cost of custom...

7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ... manipulation. Nature Reviews Genetics, 24(3), Article 3. https://doi.org/10.1038/s41576-022-00541-1 Kim...

8. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...images of their proteins. Since the 1980’s, journal articles have been filled with impressive, full-color images...

9. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   Blog Post

   ...a gRNA expression cassette in one single viral particle. The earliest explorations of the use of AdV for...

10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    ...Despite multiple studies in high profile journal articles, many researchers remain unaware that EGFP/GFP...

11. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...depositor including genbank files and protocols here. Articles Citing this Plasmid: This is a relatively new ...

12. Light Sheet Fluorescence Microscopy

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    Blog Post

    ... generated by sunlight to observe single gold particles with diameters less than 4nm. Lateral view of ...

13. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ... really inspirational. They share experiences, articles about women in science, and the sometimes stupid...

14. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post

    ... cells will be transduced with multiple viral particles and labeled a variety of colors (figure 4b). But...

15. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    ...protocols outlining these steps have been made publicly available by both the Jacob Corn and Alex Schier...

16. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...on constructing fusion proteins, check out our article: Fluorescent Proteins 101: GFP Fusion Proteins ...

17. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...research? Cary Valley contributed to updating this article. References Cox, David B.T., et al. “RNA editing...

18. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...breadth of work from a field (perhaps even two articles per meeting) would be useful. On the other hand...

19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ...treatment could be an effective strategy. (This article on fixation has some more examples to help choose...

20. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post

    ...a collaborative database for genomics. In this article, Quilt CTO Aneesh Karve, shows how to design experiments...