We narrowed to 396 results for: ICL
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TypeBlog Post...Bacterial Transcription Terminators: The RNA 3′-End Chronicles. Journal of molecular biology. 2011. PMID: 21439297...
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Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...JulietAdvocSci. Further Reading Find more Career articles on the Addgene Blog Read Joanne Kamen's Management... -
Lentiviral Vector Guide
TypeGuide...packaging genes gag , pol , and env to create viral particles. Wild-type lentiviruses additionally require the...lentivirus production — that is, creation of viral particles that could infect cells and further replicate ...concentrate. You can use crude or concentrated viral particles to transduce the cells of interest, either with...types and enhances the stability of the viral particles. However, VSV-G is toxic under long-term, constitutive...cell genome but cannot produce functional viral particles without the genes encoded in the envelope and ...the resulting construct is packaged into a viral particle, it is not possible for viral vectors to replicate...Precursor structural protein of the lentiviral particle containing matrix, capsid, and nucleocapsid components... -
Hot Plasmids: Winter 2025
TypeBlog Post...preps in the repository through our Hot Plasmids articles. Here's what you'll find in this post: Optimized... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...there are many cases where the specific viral particles you need are not available, or the cost of custom... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...where her research is focused on engineering nanoparticles as drug delivery systems. She is also a NASA... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post... manipulation. Nature Reviews Genetics, 24(3), Article 3. https://doi.org/10.1038/s41576-022-00541-1 Kim... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...images of their proteins. Since the 1980’s, journal articles have been filled with impressive, full-color images... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...a gRNA expression cassette in one single viral particle. The earliest explorations of the use of AdV for... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...Despite multiple studies in high profile journal articles, many researchers remain unaware that EGFP/GFP... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...depositor including genbank files and protocols here. Articles Citing this Plasmid: This is a relatively new ... -
Light Sheet Fluorescence Microscopy
TypeBlog Post... generated by sunlight to observe single gold particles with diameters less than 4nm. Lateral view of ... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post...educational resources, such as protocols, Plasmids 101 articles on various plasmid features, and reference tables... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post... really inspirational. They share experiences, articles about women in science, and the sometimes stupid... -
Gamma-Retroviral Vector Guide
TypeGuide... change, the supernatant containing the viral particles is removed and stored or centrifuged to concentrate...types and enhances the stability of the viral particles. However, VSV-G is toxic under long-term, constitutive...Gamma-retroviral vectors have been shown to be safe delivery vehicles and provide stable integration of the intended...cell genome but cannot produce functional viral particles without the genes encoded in the envelope and ...that resulting construct is packaged into a viral particle, it is not possible for viral vectors to replicate...Precursor structural protein of the retroviral particle containing matrix, capsid, and nucleocapsid components... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...on constructing fusion proteins, check out our article: Fluorescent Proteins 101: GFP Fusion Proteins ... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...research? Cary Valley contributed to updating this article. References Cox, David B.T., et al. “RNA editing... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...breadth of work from a field (perhaps even two articles per meeting) would be useful. On the other hand... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...protocols outlining these steps have been made publicly available by both the Jacob Corn and Alex Schier... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post... cells will be transduced with multiple viral particles and labeled a variety of colors (figure 4b). But...