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We narrowed to 933 results for: Kin

Showing: 321 - 340 of 933 results

  1. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications...others will avoid it like the plague. Instead of looking for The One Method to Rule Them All, consider the...embark on a project, do your best to understand what kind of resources - including your time - will be required...Podcasts Medium Easy for audience to engage while multitasking. Easy to host guests remotely. Easy access from...things to be physically interacted with. Public speaking is a common fear. Can be discouraging to build...

  2. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...in “crispr” and see what comes up. Before even clicking the ‘Search’ button a new content-aware suggestion...jump directly to the type of information you are looking for in our catalog or educational resources: plasmid... your lab. Instead of starting a new search or clicking through to a different part of the website, you...been deposited? In this example we’re going to be looking for recombinant monoclonal antibody plasmids from...finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where...functionally (e.g. Base Editing) and by target organism. Clicking through to RNA Editing brings us to a page with...

  3. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...employees spent this remaining time by elaborately pranking each other, dressing in costume, and holding competitions...the question, “What’s your favorite part about working at Addgene?” Most Addgenies reply with an enthusiastic...collaborators have completed their work thoroughly. It’s kind of a chicken and egg situation- do we work so hard...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all ... To keep the organizational side of things from taking too much of your time, scroll down for some tips...fall into that rut! We’ve done everything from kayaking, to field day, to relay triathlons, to 5Ks. Many...

  4. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ....g. 5’-GGAG and 3’-TACT flanking promoter parts, 5’-AATG and 3’-GCTT flanking CDS parts, etc). These overhangs... Level 1 vector with a promoter and terminator flanking the CDS, and a Level 2 multigene vector with two...depend on compatibility of four-base overhangs flanking each genetic part. (A) Example Level 0 assembly...Level 0 vectors depends on the four-base overhangs flanking each part, as they need to match sequentially ...and request your sequence with the appropriate flanking restriction sites and overhangs already added....

  5. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...modulating the production of the pro-inflammatory cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN... circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex process...reflect elements of drug addiction and craving (‘seeking drugs’).  What’s involved in locomotor sensitization...protected) rather than being out in the open (risky). Taking advantage of these characteristic traits, rodents...reduced anxiety levels and a higher tendency for risk taking. These animals also show signs of neuroinflammation...

  6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...consider a dual-sgRNA system. This system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic...deleting the catalytic residue of a protein or knocking out a very small peptide. In these cases, you ...xCas9 has a PAM requirement of NG, GAA, or GAT, making it a more flexible cutter with the added bonus ... You can achieve your precise edit without even making a break.  Base editors are dead Cas9 (dCas9) or...sites was sufficient to repress transcription by blocking its initiation. In mammalian cells, robust repression...interest. dCas9-based repressors are useful when knocking out a particular gene isn’t feasible. For example...

  7. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...chat is a convenient alternative for asking a quick question or making initial contact, but is less suitable...to look forward to in a technical support role Talking to customers everyday, it can be fascinating and...right place to live it out, but ideally without asking too many (annoying) questions. Identifying and ...understanding of when to use these communication skills by working with people from many different backgrounds and... other roles in industry. Many thanks to Eric Perkins and Stephanie Hays for images used in this post...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ..., 2020.   Scientists around the world have been making major improvements to CRISPR technology since its...blue light is readily absorbed and scattered by the skin, so it is not able to reach deeper tissues. Additionally... however, can penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST system, Ye...By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft of Cas9, the fluorescent...turn on transcription, label chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned...spatial control of Cas9 cleavage when using a photomasking technique. Last, Hemphill et al. presented data...techniques (Matony et al., 2020). Whether you are looking to activate, repress, or modify a gene, you now...

  9. Five Popular Model Organisms

    Type
    Blog Post
    ...model organism in scientific research. Yes, the same kind we use in breads and other baked goods! Yeast is... the study of neurological diseases, such as Parkinson’s disease (PD). PD is primarily characterized by...C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments over multiple...60% of human genes have an ortholog in C. elegans making it ideal to study basic molecular biology processes...resources check out Addgene’s Worm Expression page. Looking for more model organisms? Check out Part two in...From the baker to the bedside: yeast models of Parkinson's disease." Microbial cell 2.8 (2015): 262. PubMed...

  10. March for Science

    Type
    Blog Post
    ...politics? Science is an apolitical process for seeking knowledge. The process begins with a testable hypothesis...experiments can help support or reject a hypothesis. Looking at the data, scientists then revise their hypotheses...scientists who hold a doctorate degree, are currently working full-time, and have received a research grant in... of the new administration’s actions, including taking down the climate change page on whitehouse.gov,...depending on the number of people supporting it.  3) Linking scientists to one particular political party while...politicians and citizens to include science in decision making, it will be considered a success. Below are resources...

  11. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...without restructuring. Positive Points for Trainees - Taking Steps to Achieve a Successful Career Career Advice...various career opportunities through our career, networking, and management for scientists blog series. We...Session focused on finding this information and making it more easily digestible/available. What I viewed...welfare of the many researchers out there who are making great progress on some of the world’s most profound...At Addgene we hope that all parties involved in making academic research a successful and enjoyable path...Blog Post Read Our Career Blog Posts Read Our Networking Blog Posts ...

  12. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...delighted to see enterprising early career scientists taking an active part in creating their own opportunities...opportunities for career development and networking. Aside from the importance of the topics to be discussed, ...building valuable skills and meeting new people by working together on the event planning and execution. I...seeing many similar examples of science trainees taking a proactive role in their non-bench training. Grad...all. For grad students and postdocs interested in taking matters into their own hands, there are some easy...

  13. A Control for All Seasons

    Type
    Blog Post
    ...you are studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an...group of mice, the experimental group, with the new kinase inhibitor and a second group, the positive control...control group, with an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You ...experimental protocol but does not receive either protein kinase inhibitor. Since the negative control group does...variability. If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better...noise. This can be done either by modifying the blocking conditions or by substituting for a more specific...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...RNase free” and refrain from talking to friends and colleagues while working with these reagents. Other ...they are relatively small and easy to work with, making them the go-to choice for studying mammalian biology...technique is still in need of improvement. Also when working with CRISPR, one must beware that not all gRNAs...blog post. Finally, always remember that you are working with RNAs, which are prone to degradation by the..., Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing with Adeno-Associated...

  15. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    ...honing your science communications skills and networking. And on social media, you could be sharing your...Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting jobs...have to create opportunities. If you find yourself asking, "Why doesn't this product/organization/position...position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew.... Usually when I see inspiring artists who are thinking about getting into the arts (or any career to ...

  16. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ... communicate effectively is part of what makes working in a team productive. Take all the opportunities...conferences on interviewing, resume writing, and networking Practice your English if you need to, go to ESL... a content-useful website, write a blog Pursue speaking invitations by inviting others (be on a speaker...“learn the business” Attend events with local networking and trade organizations Another excellent perspective...People Wish They’d Learned Sooner by Peggy Klaus Looking for a mentor or want to start a peer mentoring ...

  17. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...offers all of those same advantages to researchers working in plant model systems. “The cloning is all done...trade Lohmann does have one special tip for anyone looking to try this at home in their own lab: be very careful...along at 99 percent efficiency, simply stopped working. They finally traced the problem back to the endonuclease...in the process. “We had several motivations for making GreenGate available through Addgene,” Lohmann said...system, the more building blocks will be available, making it easier for us to do our work.” References 1...

  18. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ... Something that I struggle with as I’ve been thinking about my career options is being an overthinker...overthinker. Being an overthinker can make me scared of taking risks, afraid of leaping forward but I have learned...in a life sciences career but life has a way of making unexpected decisions. As someone who was not necessarily...what my Instagram posts would look like. While working remotely was a different experience, It was helpful... to their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s Science...

  19. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    ...Mentor – what do I do now? What are the benefits of taking on my own Mentee? Q1. What makes a good science...participants had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing...Mentor is often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela...on the blog “Tenure, She Wrote” is titled, “What Kind of Mentor Do I Want To Be?” In this excellent essay...the northeast, writes a thoughtful treatise on the kind of advisor/mentor she wants to be for her new lab...

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Anterograde transport is typically used for the trafficking of organelles, such as mitochondria, as well ... dynein, while anterograde transport relies on kinesin. Different rates of speed for various forms of ...particles will infect neurons at different ratios, making these neurons fluoresce in different colors. An... The underlying mechanisms of trans-synaptic trafficking are not fully understood and may be a form of...B.T., Maguire, C.A. and Ramirez S.H. (2017). Trafficking of adenoassociated virus vectors across a model...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa... Resources on the Addgene Blog Retrograde AAV: Making the journey from axon to nucleus Rabies and neuronal...
Showing: 321 - 340 of 933 results