We narrowed to 464 results for: LARGE
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TypeBlog Post...have some disadvantages. These elements are quite large (500-600 bp) and may take up precious space in viral...
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Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...transcription factors, therefore promoters play a large role in determining where and when your gene of ... -
Using AAV for Neuronal Tracing
TypeBlog Post...using these viruses to mark neuronal connectivity a large majority of neuronal cells are labeled overtime ...based on its unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with AAV vectors... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method had a reprogramming...research and clinical trials, as it allows access to a larger number of willing participants. While fibroblasts... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...Measuring the Activity of Protein Variants on a Large Scale Using Deep Mutational Scanning.” Nature Protocols... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...lead to asymmetrical plasmid loss of the usually large, low copy virulence plasmids. This strategy has ...partitioning machinery away from a larger, low copy plasmid and the larger, low copy plasmid is more likely... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post... linearized pEcgRNA backbone can be prepared in large amounts and frozen for future use or directly ligated... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins or selection... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...difficult. The first-generation mARGs also had a large genetic footprint (two cassettes + a booster cassette... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...by adding a nuclear localization sequence (SV40 Large T nuclear localization sequence) and expressing ... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post... 3. Mashiko, Daisuke, et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas ... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...institution/funding source. Major equipment includes large centrifuges, sequencers and analyzers, and any ultra... -
Plasmids 101: Biotinylation
TypeBlog Post...bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin complexes bound to your... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...something should be communicated is necessary in a large organization, because confirming that every person... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...blocks for engineered proteins. This flexibility is largely due to scFvs being smaller than antibodies. scFvs... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...double-stranded DNA transgene is incorporated into a large complex of both viral and cellular proteins and ...enzymes that are proteolytically processed from the larger protein: PR (protease), RT (reverse transcriptase... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...cells compared to dead cells. As a result, the largely negative live cells can still be easily gated on... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...Both subunits have short intracellular ends and large ectodomains containing ligand-binding heads and ... -
CRISPR 101: Off-Target Effects
TypeBlog Post...frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and targeting... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...changes in enzyme function can lead to surprisingly large fitness effects during adaptive evolution of antibiotic...