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We narrowed to 356 results for: SAM activators

Showing: 321 - 340 of 356 results

  1. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...pseudotype To pseudotype your viral vector, you use the same protocol for generating lentivirus, but you use ...Different pseudotyped viruses are differentially inactivated by nonspecific complement mechanisms in serum...anti-alphaGal antibodies. The VSV-G envelope protein is inactivated by serum regardless of the producer cell type...high concentrations as described above and is inactivated by the complement system in serum (DePolo et ...Vector Particles Produced in Human Cells Are Inactivated by Human Serum. Molecular Therapy 2:218–222 ....

  2. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... DIY Biology and, on occasion, would meet in the same space where BosLab exists today. Back then, it consisted...saw the potential in the space and decided to reactivate it. We started out by holding a few journal club... getting the community to collect various soil samples, extract DNA, sequence it, and analyze the data... characterize the microbiomes of the different samples. The goal is to not only learn something about ...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...gene activity. Latent infection A state where a pathogen is present in an organism but not actively causing...is crucial for efficient AAV replication as it activates the necessary cellular machinery to produce large...non-dividing cells while reducing the risk of immune activation, both key advantages of rAAV vectors. AAV Types...Optogenetics Biological technique to control the activity of cells, often neurons, with light. Pseudotyping... new window) McCarty, D. M., Monahan, P. E., & Samulski, R. J. (2001). Self-complementary recombinant ...Link opens in a new window) Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated virus serotypes...in cis refers to genetic elements located in the same plasmid as the gene of interest. in trans In the...

  4. Cloning

    Type
    Guide
    ... Cloning (LIC) relies on the 3'-5' exonuclease activity of T4 DNA polymerase. An exonuclease is an enzyme...strand. In LIC, the T4 DNA polymerase’s exonuclease activity creates “chewed-back” overhangs of 10-12 base ...this opportunity, T4 will resume its polymerase activity, add back the free base, and become stuck at this...side of it into any plasmid that has the same sites in the same orientation within its multiple cloning...

  5. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...strategies to investigate a gene's function is to inactivate, or "knockout", the gene by replacing it or disrupting...describe the techniques and the plasmids used to inactivate specific genes in mammalian cells. Despite the...one of its exons has simply been replaced with the same sequence flanked by LoxP sites while the resistance... are easier to use and are more efficient at inactivating genes. CRISPR can target a genomic sequence ...

  6. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ... the interactive photo graffiti wall. I had a lot of fun playing the prototype of an interactive game ...playing all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you might have...

  7. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...last year, Verkhusha added two far-red light photoactivatable (PA) near-infrared fluorescent proteins (FPs...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...751-4. doi:10.1038/nmeth.2521) Far-red light photoactivatable near-infrared fluorescent proteins engineered...

  8. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ... are trying to disrupt or delete, which both inactivates the gene and acts as a marker for the mutation...of experiments it is best practice not to use the same resistance cassette for the mutation and for plasmid...cassettes and your gene of interest is cloned into/inactivates or completely removes (in the case of Gateway...

  9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...approach – co-CRISPR from Craig Mello’s lab – used inactivation of the unc-22 gene as their selection marker...Drosophila S2 cells (Böttcher et al., 2014), inactivation of non-homologous end-joining results in a further...changes 5’ to the PAM, sequences corresponding to the same strand as the PAM produced a higher editing efficiency...elegansGenome with Lethal Mutation Co-Conversion and Inactivation of NHEJ Repair. Genetics 199:363–377 . https...

  10. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... have a higher transduction efficiency, i.e. the same number of AAV particles is able to infect more cells... protects them from antibody recognition and inactivation (György et al., 2014), but scientists have yet...difficult to reach, and are more resistant to inactivation through anti-AAV antibodies. References György...

  11. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...classic approach for studying protein turnover is radioactive pulse-chase experiments. However, these experiments...require radiolabeled amino acids. The use of radioactive materials is highly regulated, requires designated... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific...

  12. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...This can differ between fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-...described by a mono-exponential decay (analogous to radioactive decay). Consequently, the intensity decay is ...comparison of several fluorescent proteins of the same spectral class on the microscopy system and in the...

  13. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...your poster. While a poster generally contains the same sections as a primary research article, it’s important...can be accomplished with an exciting title and attractive figures. Next, the poster should be legible from...four column style, and in all cases the idea is the same; the reader will navigate the poster from column...

  14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be... Central PMCID: 5088052. 3. Lentz TB, Gray SJ, Samulski RJ. Viral Vectors for Gene Delivery to the Central...

  15. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...paying bills and having a life outside my job. The same can be said about teamwork, communication, writing... every piece of work you touch, and be a more attractive candidate for that ideal job. How can you identify...process. You stepped up and got your team on the same page. This came naturally to you. How is this new...

  16. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...Additional uses The cover letter is a great place to proactively address questions about your application. These...Structure of a cover letter Cover letters follow the same format regardless of whether you’re in academia,...requirements, and address any unusual circumstances or proactively answer questions the hiring manager may have....

  17. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging...super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes...smFPs retain their ability to be detected after sample preparation better than standard proteins tags ...

  18. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by species and route ...doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated virus serotypes....2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R. J. (2006). Α2,3 and α2,6 N-Linked Sialic Acids...

  19. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...Green Lab website, but if you don’t see one, be an ACTivist and encourage companies to obtain these labels...cytometers. These fluidic machines handle biological samples and are cleaned between each use. You wouldn’t ...
Showing: 321 - 340 of 356 results