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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 570 results for: SHA

Showing: 321 - 340 of 570 results

  1. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...binding pocket of an engineered receptor that change shape with light exposure to activate them. PORTLs use... Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H....

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...optimizing!  Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ....org/10.1038/s41434-019-0075-6 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...blocking for 1 h at room temperature with gentle shaking, gently wick off excess liquid from each membrane...start) and add your membranes to the solution, shaking gently for 1 h at room temperature. This will allow...

  6. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...course on yeast display for antibody discovery, to share their expertise on the topic. Evolving the future...with verifiable data is critical, especially when sharing with other scientists. Addgene partners with IPI...

  8. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ... focus). This means that the images will appear sharp without interference from blurry out-of-focus light... lightsheet that projects a number of thin cone-shaped beams into the sample. These beams interfere to...

  9. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...how science can be accelerated immensely when we share knowledge and reagents. I have not intentionally...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... also known as immunoglobulins, are ~150 kDa, Y-shaped proteins that are both a natural part of the immune... the Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies...

  13. Deep Dive: qPCR

    Type
    Blog Post
    ...and reliable qPCR data, you’ll of course want to share it so others can provide you with the highest form....2018.12.002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number...

  15. New Western Blot Protocol Video!

    Type
    Blog Post
    ... Addgenie Meghan and Abi run a Western blot and share tips for this tricky application.    You can check...

  17. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...isn't your platform, all of our videos will also be shared on our @addgene Instagram account, or you can watch...

  18. 2022 Depositor Week

    Type
    Blog Post
    ...revamped Data Hub! The community needs more data-sharing heroes like you.)  ...

  19. CRISPR Guide Update

    Type
    Blog Post
    ...With this update, we have added new sections to share and describe some incredible new CRISPR technologies...
Showing: 321 - 340 of 570 results