We narrowed to 570 results for: SHA
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TypeBlog Post...binding pocket of an engineered receptor that change shape with light exposure to activate them. PORTLs use... Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H....
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Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...optimizing! Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ....org/10.1038/s41434-019-0075-6 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...blocking for 1 h at room temperature with gentle shaking, gently wick off excess liquid from each membrane...start) and add your membranes to the solution, shaking gently for 1 h at room temperature. This will allow... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...course on yeast display for antibody discovery, to share their expertise on the topic. Evolving the future...with verifiable data is critical, especially when sharing with other scientists. Addgene partners with IPI... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post... model. For example, in a recent study by the Wickersham lab (Lavin, TK. 2020) it was determined that ...10.1089/hum.2019.277 Lavin TK, Jin L, Lea NE, Wickersham IR (2020) Monosynaptic Tracing Success Depends... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post...sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches and most smaller...hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...correct orientation to be transcribed (Figure 2) (Sharma and Zhu, 2014). How does FLEx enable strong opsin...biotechnology 21.5 (2003): 562. PubMed PMID: 12665802. SSharma, Suveena, and Jinfang Zhu. "Immunologic applications... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...how science can be accelerated immensely when we share knowledge and reagents. I have not intentionally...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...:19169259. PubMed Central PMCID:PMC2901231. 3.Verkhusha, Vladislav V., and Alexander Sorkin. "Conversion...25581799. PubMed Central PMCID:PMC4344411. 13.Berlin, Shai, et al. "Photoactivatable genetically encoded calcium... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post... focus). This means that the images will appear sharp without interference from blurry out-of-focus light... lightsheet that projects a number of thin cone-shaped beams into the sample. These beams interfere to... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post... also known as immunoglobulins, are ~150 kDa, Y-shaped proteins that are both a natural part of the immune... the Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...handles the design of sgRNA and represents the lion’s share of the currently available tools (we will focus ...the user to save the results and return later or share them. Community exclusivity: Different communities... -
Deep Dive: qPCR
TypeBlog Post...and reliable qPCR data, you’ll of course want to share it so others can provide you with the highest form....2018.12.002.Teo IA; Choi JW; Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...Gram-positives and ICEbs1 in Gram-negatives) but share the common CRISPR-dCas9 system for targeted repression...mbio.00019-20 Singh AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating... -
New Western Blot Protocol Video!
TypeBlog Post... Addgenie Meghan and Abi run a Western blot and share tips for this tricky application. You can check... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...We are very excited to share the latest edition of our Viral Vectors 101 eBook! This practical guide... -
Antibodies 101 Series on TikTok
TypeBlog Post...isn't your platform, all of our videos will also be shared on our @addgene Instagram account, or you can watch... -
2022 Depositor Week
TypeBlog Post...revamped Data Hub! The community needs more data-sharing heroes like you.) ... -
CRISPR Guide Update
TypeBlog Post...With this update, we have added new sections to share and describe some incredible new CRISPR technologies... -
Introducing the Addgene Antibodies 101 eBook!
TypeBlog Post...Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition...