Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 672 results for: abo.1

Showing: 321 - 340 of 672 results

  1. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...distinguish between your two targets.    Figure 1: In the isotype conversion process the variable regions...natural isotype to that of a different group (Fig. 1). This process, called isotope conversion, gives users...flexibility needed for multiplexing. In the case above, you can’t costain with your desired antibodies ...multiplexing with chimeric antibodies. In the case above you should use an anti-rat IgG1 secondary antibody...this blog post has answered all of your questions about the utility of isotype conversions and chimeric ..., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., &...

  2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...guide RNA (sgRNA), provided it meets two conditions:  1) The sequence is unique compared to the rest of the...bystander bases” if you choose this system.  Fig. 1: Cytidine deamination takes place on the free strand...temperature-controlled genome editing. Nat. Commun. 2017 81 8, 1–9 (2017).Walton, R. T., Christie, K. A., Whittaker... 101 Series Learn about the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled Library...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...In vitro screening for the best gRNA Figure 1: Validation of double strand break (DSB) mediated ...as control (Cetn1/sgRNA1), 2; darker than control, 1; very dark). Brighter fluorescence indicates a more...and greatest in scientific content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target effects...the pX330 plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014...Engineering with CRISPR Validate Your Genome Edit Learn about Non Homologous End Joining and Homology Directed...

  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...Check out Addgene's New Viral Service! References 1. Hay, R.J., Macy, M.L. & Chen, T.R. “Mycoplasma infection...contaminations.” Methods in Molecular Biology 946 (2013). 1-13. Pubmed PMID: 23179822. 5. Segura, M.M., Kamen,...Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...Get Tips for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide...

  5. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...oligodeoxynucleotides)) are commonly used for smaller modifications (~1-50 base pairs). Small edits require as little as 30...Easi-CRISPR was shown to increasing editing efficiency from 1-10% with dsDNA to 25-50% with ssODNs in mouse models...ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (2017). PubMed.  Additional Resources on the Addgene...Featured Topic Page Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips ...

  6. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...sequences are inserted into the genome. Figure 1: Self-excising cassette schematic from Dickinson et...):1028-34. doi: 10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at ...just one injection step! The entire protocol takes about 2-3 weeks but requires less than eight hours worth... this species, namely, the robust HDR mentioned above, an accessible germline, and multiple selectable...modular system that requires very little hands-on labor and could facilitate the tagging of every protein...

  7. Plasmid Cloning by PCR

    Type
    Blog Post
    ...has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million bp depending on the polymerase...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time...Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch Up on All Things ...

  8. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...pipetting, he can be seen daydreaming about sunny days in the UK.   References 1. Bressan RB, Dewari PS, Kalantzaki...the tag sequence flanked by homology arms (Figure 1). In the first step of optimization, we started off...form the ribonucleoprotein complex (csRNP, Figure 1). We use in-house purified Cas9 protein, which, in...standard immunocytochemistry (ICC) assays.  Figure 1: Three ingredients of epitope-tagging experiments....ChIP-seq methods provide a wealth of information about how protein networks work in cells. A major bottleneck...two-part cr/tracrRNAs for CRISPR experiments (in collaboration with Integrated DNA Technologies, USA). These...commercial suppliers without investing time and labour on reagent preparation in the lab. Figure 3: csRNP-assisted...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus genome. ...gamma-retroviruses ranges from 9–11 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...gamma-retroviral vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors...of plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...packaging methods . The three plasmids described above (envelope, packaging, and transfer) are co-transfected... Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S., Su, L.,...

  10. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...microbial research like metabolism. Transport, biofilms, and much more. Because of it’s BSL-1 status, E. coli ...oxygen. Additionally, its status as a Biosafety Level 1 microbe makes it a perfect candidate for introductory...with superior performance. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-04899-x Yokoyama... more about model organisms in the first Five Popular Model Organisms blog post Read more about plasmids...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive...

  12. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ... There are two methods of neuronal tracing (Fig. 1): one to visualize neurons that send information to...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer AAV variant... Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service Resources...We often think about the brain’s function in terms of its regions. But equally important is the way the...head bone’s connected to the neck bone,” but with about a hundred billion neurons and six hundred trillion...

  13. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...restriction digest and colony PCR.   Fig. 1: (A) Schematic of cloning, expression and validation... doi: 10.1371/journal.pone.0038313. Epub 2012 Jun 1. PMID: 22675541; PMCID: PMC3365890.... called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed through NABOR are recombinant...troubleshooting methodologies drew me to learn more about the process that was used to create this important...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

  14. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...immunogenicity score (Mattei et.al., 2022).    Figure 1: Generation of immunogenicity scores by predictive...antibodies for the treatment of diseases. J Biomed Sci 27, 1 (2020). https://doi.org/10.1186/s12929-019-0592-z  ...determining the T-cell epitopes that will likely bring about unwanted immunogenicity. The 3-D models can be applied...limitations. Using methods together can inform decisions about in vivo application and clinical testing. This would...to drugs: adverse reaction terminology. WHO Collaborating Centre for International Drug Monitoring, Uppsala...

  15. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...activity degrades the displaced strand.   Figure 1: EvolvR consists of an error-prone polymerase (PolI...mutation rate and extend the editing window of EvolvR: (1) two additional mutations to PolI3M generated PolI5M...blog Learn about other CRISPR methods for bacterial genome engineering   Learn more about lambda red ... genetic variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications...

  16. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...a glossary at the end of this post! Figure 1: Base transitions are edits between the purine bases...uracil DNA glycosylase. Nature Communications, 15(1), 4897. https://doi.org/10.1038/s41467-024-49343-5...uracil-DNA glycosylase. Nature Communications, 15(1), 6397. https://doi.org/10.1038/s41467-024-50012-w...In our last post, we talked about the first base transversion editors: CGBEs, or C → G Base Editors. ...apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David Liu and Dali Li labs reported... CRISPR 101: Cytosine Transversion Editors Read about four base editor reporters Resources on Addgene.org...

  17. Evolution of Lab Techniques

    Type
    Blog Post
    ...blotting, named for its inventor Edward M. Southern (1), can be used to detect DNA identity, size, and abundance...hours at low cost with restriction mapping (Figure 1). Though some labs may prefer to send DNA directly... serotonergic system and behavior.     References 1. Southern, Edwin Mellor. "Detection of specific sequences...abundant in a sample. Whereas Southern blotting is labor intensive and requires a large amount of high-quality...DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...PMC4411503. Resources on the Addgene Blog Learn More about Restriction Cloning Get Tips on Analyzing DNA Sequencing...

  18. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...of an industry H-1B need to be aware of the April 1 deadline for companies to submit an application on...and non-profit organizations do not have the April 1 H-1B queue process or quota like industry, these organizations... National Interest Waiver. The three prongs are: (1) The foreign national’s proposed work endeavor must...-1B, you need to prove 3 threshold requirements: (1) that you have international recognition for your ... data and the list goes on and on. As you think about your career (and your future in general), keep in...in this post benefit from the applicant thinking about the process early, keeping good records, and planning...writing, compliance, core facility management, collaboration management, grant administration, clinical operations...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...overcomes many of those limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule...GTPase Signaling Toolbox. ACS Synthetic Biology, 11(1), 515–521. https://doi.org/10.1021/acssynbio.1c00604... Check out our Optogenetics Guide to learn more about how these modules work, and read on to learn how...to the cell membrane isn’t powerful enough, what about altering the chemical composition of the membrane...

  20. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...screening blog post for more information.   Figure 1: Result of a blue-white screen. Image from Stefan ...Because Sanger sequencing can usually sequence just 1 kb of DNA, custom primers are especially helpful in...often relies on antibiotic resistance. But what about identifying clones that contain your desired insert... restriction digest. The key is to be strategic about your enzyme choices. You’ll want to pick enzymes... for your PCR.  Find the details and learn more about colony PCR in this blog post.   Figure 2: ...Sanger sequencing or the other techniques mentioned above. In molecular biology, cloning a gene into your ... and tips Resources on Addgene.org Learn more about Sanger sequencing Read the Molecular Biology Reference...
Showing: 321 - 340 of 672 results