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We narrowed to 58 results for: abo.1

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Showing: 1 - 20 of 58 results

  1. Immunology Research Plasmids and Resources

    Type
    Collection
    ...IGHDY1 IGHD1-1 immunoglobulin heavy diversity 1-1 IGHD11 IGHD1-14 immunoglobulin heavy diversity 1-14 (non-...IGHV6-1 immunoglobulin heavy variable 6-1 IGHV61, VH IGHV7-4-1 immunoglobulin heavy variable 7-4-1 IGHV7... TAPBP-R, TAPBPR THBS1 thrombospondin 1 THBS, THBS-1, TSP, TSP-1, TSP1 TRPC4AP transient receptor potential...variable 2-8 IGLV28, V1-2 IGLV3-1 immunoglobulin lambda variable 3-1 IGLV31, V2-1 IGLV3-10 immunoglobulin lambda...NCC-1, NCC1, SCYA13, SCYL1 CCL14 chemokine (C-C motif) ligand 14 CC-1, CC-3, CKb1, FLJ16015, HCC-1, HCC... 3 G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3 CCL3L1 chemokine (C-C motif) ligand 3-like 1 464.2, D17S1718...GPD, GpFy, WBCQ1 DEFA1 defensin, alpha 1 DEF1, DEFA2, HNP-1, HP-1, MGC138393, MRS DEFA3 defensin, alpha...

  2. Ras Pathway

    Type
    Collection
    ... dependent protein kinase 1 PEBP1 Phosphatidylethanolamine binding protein 1 PIK3 Catalytic Subunits PIK3CA...isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase 1 catalytic subunit alpha PREX2...family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 RHEB Ras homolog enriched in ...protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein 1 SCRIB Scribbled planar cell...guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return to top Resources RAS Pathway...Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid 2 like 2 NFKB1 Nuclear... kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3 PAK4 p21 protein (Cdc42/Rac)-activated...

  3. Recombinases AAV Preps

    Type
    Collection
    ...Cre.SV40 CamKII none 1, 5, 9, rg* James M. Wilson 105537 pENN.AAV.CMVs.Pl.Cre.rBG CMV none 1, 2, 5, 8, 9, rh10... none 1, 5, 8, 9, rg* Esteban Engel , Alexander Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Connie...Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn none 1, 2, 5, ...-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, 2, 5, 8, 9, rg*, PHPeB...mCherry (not a fusion tag) 1, rg* Karl Deisseroth 55637 pAAV-EF1a-Flpo EF1a none 1, 2, rg* Karl Deisseroth...pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none 1, 2, 5, 8, 9, rg* Li Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Connie Cepko 183412...pAAV-EF1a-iCreV EF1a none 1, PHPeB Hongkui Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Hongkui Zeng 140137...

  4. Optogenetics AAV Preps

    Type
    Collection
    ...Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg...Constitutive 1, 5, rg* Edward Boyden 59171 pAAV-Syn-ChrimsonR-tdT Syn ChrimsonR tdTomato Constitutive 1, 5, 9...Constitutive 1, 5 Ofer Yizhar 198511 pAAV_hSyn-DIO-PdCO-mScarlet-WPRE Syn PdCO mScarlet Cre dependent 1, 5 Ofer...pAAV-S5E2-ChR2-mCherry nEF ChR2 mCherry Constitutive 1, 9, PHPeB Jordane Dimidschstein 137142 pAAV-nEF-Con...-WPRE-HGHpA EF1a ChR2/H134R mCherry Cre dependent 1, 5, 8, 9, rg* Karl Deisseroth 20298 pAAV-EF1a-double...EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP Cre dependent 1, 5, 9, rg*, PHPeB Karl Deisseroth 26969 pAAV-CaMKIIa-hChR2...pAAV-CaMKIIa-hChR2(H134R)-EYFP CaMKII ChR2/H134R EYFP Constitutive 1, 2, 5, 9 Karl Deisseroth 26973 pAAV-hSyn-hChR2(H134R...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...accumulation in bacteria. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose Green fluorescent MBP-based...dynamics of central redox metabolism across eukaryotes. Nat Commun. 2024 Dec 19;15(1):10704. Markus Schwarzländer...of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881. Adam Cohen Calcium GCaMP5 genetically encoded...in High-Ca 2+ Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(16)30163-5. Teresa Alonso , Javier... intracellular calcium. Nat Commun. 2021 Dec 9;12(1):7159. Dorus Gadella Calcium Teal genetically encoded...orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer Calcium Orange fluorescent calcium...dynamics in mitochondria. Sci Rep. 2020 Feb 18;10(1):2835. Masamitsu Iino Calcium Red calcium sensor FRCaMPi...

  6. mTOR Pathway

    Type
    Collection
    ...rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase...translation initiation factor 4E, binding protein 1 Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation...RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras homolog enriched in brain S6K Also known ...Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor...protein p53 PDK1 Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB PIK3CD PIK3CG...complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return to top Resources Cancer Pathway ORF Kit (Sabatini... the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein...

  7. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...190293 Anti-AMIGO-1 [L86/14] AMIGO-1 Mouse Mouse IgG2a 190294 Anti-AMIGO-1 [L86/2] AMIGO-1 Mouse Mouse IgG2a...ATPase 1 [L60/4R-2b] Copper ATPase 1 Human Mouse IgG2b 206648 Anti-AMIGO-1 [L86/33R-2b] AMIGO-1 Mouse ...channel [L6/60R-1] Slo1 K+ channel Mouse Mouse IgG1 206695 Anti-AMIGO-1 [L86/33R-1] AMIGO-1 Mouse Mouse IgG2a...Anti-Olig1 [N149/25R-1] Olig1 Mouse Mouse IgG1 206702 Anti-Neurexin-1-Beta [N170A/1R-1] Neurexin-1-Beta Human Mouse...222160 Beclin-1 [N248A/15R] Beclin-1 Mouse Mouse IgG2a 222161 Beclin-1 [N248A/22R] Beclin-1 Mouse Mouse ...Mouse Mouse 190534 Neurexin-1-Beta scFv [N170A/1] N170A/1 scFv Neurexin-1-Beta Human Mouse 190535 PSD-...Mouse 206755 IP3 receptor, type 1 scFv [L24/1] L24/1 scFv IP3 receptor, type 1 Rat Mouse 206756 VSP scFv [...

  8. Chemogenetics AAV Preps

    Type
    Collection
    ...hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 44362 pAAV-hSyn-DIO-hM4D...hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...mCherry hM3D(Gq) - Activation mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan Roth 50475 pAAV-hSyn-hM4D(Gi...mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan Roth 154867 pAAV-hSyn-fDIO-...mCherry hM3D(Gq) - Activation mCherry fusion none 1, 2, 5, 8, 9 Bryan Roth 50477 pAAV-CaMKIIa-hM4D(Gi)...mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 2, 5, 8, 9 Bryan Roth 75033 pAAV CD68-hM4D(Gi)-mCherry...mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 9 Bryan Roth 83896 pAAV-hDlx-GiDREADD-dTomato-Fishell...

  9. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...library as GST-fusion peptides (“Mode #1” expression). The Mode #1 phosphosite library (Pooled Library #111704...References & Protocols Additional Resources Figure 1: SepRS adds a phosphoserine to tRNA^Sep with anticodon...standards for quantitative assessments. The Mode #1 phosphosite library can be generated with either phosphoserine...phosphorylation-dependent protein-protein interactions Figure 2: Mode #1 and Mode #2 phosphosite library configuration for ...Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also be synthesized using ...iSPI_pSer_Subpool#2 Pooled Library 188526 iSPI_pSer_Subpool#1 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid... #2 Library (14-3-3β) Pooled Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...AdEasier®-1 cells (strain) - Bacterial strain that contains AdEasy®-1 plasmid...N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...expression under a CMV promoter pAdEasy®-1 - Recombine plasmids from the shuttle... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 ...lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning and...

  11. Fluorescent Proteins: FRET

    Type
    Collection
    ...EYFP 434 0.41 527 67,000 0.67 4.8 1.5 pmECFP-1 , pmEYFP-1 , pECFP18aaEYFP mCerulean mVenus 433 0.49 527...nm), ε A : Acceptor exctinction coefficient (M -1 cm -1 ), QY A : Acceptor quantum yield, R 0 : Förster...emission and acceptor excitation spectra (×10 15 M -1 cm -1 nm 4 ). Values obtained from FPbase.org FRET calculator... the donor lifetime. FRET is often used to study (1) protein-protein interactions where each protein is...intramolecular or unimolecular FRET). Learn more about FRET on our blog and explore the tables below for...fluorophores via HaloTag or SNAP-Tag . Read more about self-labeling tags on our blog . Luciferase can ...

  12. AAV Packaged on Request

    Type
    Collection
    ...Small NEW 2 × 100 µL aliquots 0.2 mL 4 × 10 12 GC/mL* 1 × 10 13 GC/mL* Medium 10 × 100 µL aliquots 1.0 mL ...to 10 weeks. This breaks down as follows: Request 1–2 days Look for the banner on an eligible plasmid ...place your request. We will send you an email within 1-2 days with a response. Most requests will be approved... we can begin processing your order. MTA Approval 1+ business days, varies by requesting organization ...scientist through an MTA. This process typically takes 1–4 days, depending on your institution. AAV Production...information before choosing a serotype? Learn more about AAV serotypes and tissue tropism on Addgene's blog...Additional Resources Viral Vector Production Learn about how we produce high-quality viral vector preps at...

  13. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR systems, the most abundant type found...bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared to their...still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with ...tables below for CRISPR Cas3 plasmids. To learn more about Cas3 and other CRISPR topics, read our CRISPR Guide...

  14. Adenovirus Plasmids

    Type
    Collection
    ... Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains the pAdEasy-1 (Addgene #16400) plasmid Vogelstein...production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use in AdEasy™ System Vogelstein...our adenoviral vector guide for more information about adenoviral components, generations, viral production...

  15. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... orientation of each target site (see Figure 1). Figure 1: Recombination outcomes are determined by target...minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and Flp Recognition Sites. Controlling...target site sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-, Flp-, or...Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome...recombinase enzyme is present. Read on to learn about the different ways that Cre-lox and other site-specific...targeted neuronal populations. Read our blog post about how to minimize leakiness in experiments using AAV...

  16. Lentiviral Prep Service

    Type
    Collection
    ...Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding...barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells for...are reported with each shipment. For more details about our viral vector packaging service, visit our Viral...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic tool to control...skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR...though the DNA-binding profile is reversed (Figure 1). However, rtTA can induce much stronger expression...Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector with blasticidin selection...Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR from CMV...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...systems: Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how to use them...

  18. Antibody Plasmid Collection

    Type
    Collection
    ...phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression...immunostaining. Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated... system, fused to sfGFP for imaging. Learn more about antibodies and their applications in our Antibody...phage display vector system for isolation of scFv, diabody and Fab fragment libraries from chickens Methods...

  19. Brain Initiative Collection

    Type
    Collection
    ...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV2 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV5 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV8 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV9 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-AAVrg pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-PHPeB pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

  20. Zhang Lab CRISPR Page

    Type
    Collection
    ...Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6...SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide ...screens. The libraries are available in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together...):186-91. doi: 10.1038/nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids...Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids...
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